|
|
|
|
|
|
|
|
|
|
|
|
Protein Info
|
|
|
|
|
|
Short Name:
|
Chk1
|
|
Full Name:
|
Serine/threonine-protein kinase Chk1
|
|
Alias:
|
CHEK1; EC 2.7.11.1
|
|
Type:
|
Protein-serine kinase, CAMK group, CAMKL family, CHK1 subfamily
|
|
Mass (Da):
|
54420
|
|
Number AA:
|
476
|
|
UniProt ID:
|
O14757
|
|
International Prot ID:
|
IPI00023664
|
|
Sequence:
|
Retrieve full protein sequence
|
|
|
|
|
GO Terms
|
|
External Links
|
Internal Links
|
|
|
|
|
|
Cellular Component:
|
GO:0005813
GO:0000794
GO:0005654
|
Uniprot
|
OncoNet
|
|
Molecular Function:
|
GO:0005524
GO:0005515
GO:0004674
|
PhosphoSite+
|
KinaseNET
|
|
|
Biological Process:
|
GO:0000077
GO:0006281
GO:0006260
|
Phosida
|
TranscriptoNet
|
|
|
|
|
STRING
|
Kinexus Products
Checkpoint protein-serine kinase 1 S280 phosphosite-specific antibody AB-PK577#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK577#Checkpoint protein-serine kinase 1 S317 phosphosite-specific antibody AB-PK578#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK578#Checkpoint protein-serine kinase 1 S345 phosphosite-specific antibody AB-PK579#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK579#Cdc25C (205-225), KinSub - Cdc25C phosphatase (V205-R225, human) peptide; Chktide peptide - Powder PE-01ACN95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01ACN95#Chk1Selectide - Chk1 (CHEK1) protein kinase substrate peptide - Powder PE-01BGS90#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01BGS90#Checkpoint protein-serine kinase 1 (I342-T348, human) pS345 phosphopeptide - Powder PE-04AJU95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AJU95#Checkpoint protein-serine kinase 1 (R277-V283, human) pS280 phosphopeptide - Powder PE-04AJV95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AJV95#Checkpoint protein-serine kinase 1 (Y314-E320, human) pS317 phosphopeptide - Powder PE-04AJW95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AJW95
|
|
|
|
|
Info Box
|
|
A variety of parameters were considered in the selection of putative P-sites.
Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score
is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr
or P-Tyr sites. The Maximum KInase Score provides the calculated score for
highest match of 500 human protein kinases for the amino acid sequence
surrounding the target P-site as determined with Kinase Substrate Predictor V2.
The Sum KInase Score provides the additive sum of the positive individual Kinase
Substrate Predictor V2 scores from 500 human protein kinases. The Conservation
Score is the average of the percent similarity of the human P-site with the
equivalent P-site in 20 other diverse species. Click the coloured buttons below
to retrieve this and other information in the Info Box, or click the orange
buttons for relevant links. |
|
| Phosphosites | |
| |
| | | -7 | -6 | -5 | -4 | -3 | -2 | -1 | 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | | Expt. conf. | Effect | Kinase | PPase | Kinexus Products | | Ref. | Evol. | Kinase Pred. | P-site Match |
| | Site 1 | Y81 | R | R | E | G | N | I | Q | Y | L | F | L | E | Y | C | S | |  |  | | | | | |  | | |
| | Site 2 | S147 | E | R | D | N | L | K | I | S | D | F | G | L | A | T | V | | | | | | | | | | | |
| | Site 3 | T153 | I | S | D | F | G | L | A | T | V | F | R | Y | N | N | R | | | | | | | | | | | |
| | Site 4 | S212 | E | L | P | W | D | Q | P | S | D | S | C | Q | E | Y | S | | | | | | | | | | | |
| | Site 5 | Y218 | P | S | D | S | C | Q | E | Y | S | D | W | K | E | K | K | | | | | | | | | | | |
| | Site 6 | S219 | S | D | S | C | Q | E | Y | S | D | W | K | E | K | K | T | | | | | | | | | | | |
| | Site 7 | Y227 | D | W | K | E | K | K | T | Y | L | N | P | W | K | K | I | | | | | | | | | | | |
| | Site 8 | S236 | N | P | W | K | K | I | D | S | A | P | L | A | L | L | H | | | | | | | | | | | |
| | Site 9 | S251 | K | I | L | V | E | N | P | S | A | R | I | T | I | P | D | | | | | | | | | | | |
| | Site 10 | T255 | E | N | P | S | A | R | I | T | I | P | D | I | K | K | D | | | | | | | | | | | |
| | Site 11 | Y265 | D | I | K | K | D | R | W | Y | N | K | P | L | K | K | G | | | | | | | | | | | |
| | Site 12 | T279 | G | A | K | R | P | R | V | T | S | G | G | V | S | E | S | |  | | | | | | | | | |
| | Site 13 | S280 | A | K | R | P | R | V | T | S | G | G | V | S | E | S | P | | | | | |  Checkpoint protein-serine kinase 1 S280 phosphosite-specific antibody AB-PK577#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK577#Checkpoint protein-serine kinase 1 (R277-V283, human) pS280 phosphopeptide - Powder PE-04AJV95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AJV95 | | | | | |
| | Site 14 | S284 | R | V | T | S | G | G | V | S | E | S | P | S | G | F | S | | | | | | | | | | | |
| | Site 15 | S286 | T | S | G | G | V | S | E | S | P | S | G | F | S | K | H | | | | | | | | | | | |
| | Site 16 | S288 | G | G | V | S | E | S | P | S | G | F | S | K | H | I | Q | | | | | | | | | | | |
| | Site 17 | S291 | S | E | S | P | S | G | F | S | K | H | I | Q | S | N | L | | | | | | | | | | | |
| | Site 18 | S296 | G | F | S | K | H | I | Q | S | N | L | D | F | S | P | V | | | | | | | | | | | |
| | Site 19 | S301 | I | Q | S | N | L | D | F | S | P | V | N | S | A | S | S | | | | | | | | | | | |
| | Site 20 | S305 | L | D | F | S | P | V | N | S | A | S | S | E | E | N | V | | | | | | | | | | | |
| | Site 21 | S307 | F | S | P | V | N | S | A | S | S | E | E | N | V | K | Y | | | | | | | | | | | |
| | Site 22 | S308 | S | P | V | N | S | A | S | S | E | E | N | V | K | Y | S | | | | | | | | | | | |
| | Site 23 | Y314 | S | S | E | E | N | V | K | Y | S | S | S | Q | P | E | P | | | | | | | | | | | |
| | Site 24 | S315 | S | E | E | N | V | K | Y | S | S | S | Q | P | E | P | R | | | | | | | | | | | |
| | Site 25 | S316 | E | E | N | V | K | Y | S | S | S | Q | P | E | P | R | T | | | | | | | | | | | |
| | Site 26 | S317 | E | N | V | K | Y | S | S | S | Q | P | E | P | R | T | G | | | | | | Checkpoint protein-serine kinase 1 S317 phosphosite-specific antibody AB-PK578#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK578#Checkpoint protein-serine kinase 1 (Y314-E320, human) pS317 phosphopeptide - Powder PE-04AJW95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AJW95 | | | | | |
| | Site 27 | T323 | S | S | Q | P | E | P | R | T | G | L | S | L | W | D | T | | | | | | | | | | | |
| | Site 28 | S326 | P | E | P | R | T | G | L | S | L | W | D | T | S | P | S | | | | | | | | | | | |
| | Site 29 | T330 | T | G | L | S | L | W | D | T | S | P | S | Y | I | D | K | | | | | | | | | | | |
| | Site 30 | S331 | G | L | S | L | W | D | T | S | P | S | Y | I | D | K | L | | | | | | | | | | | |
| | Site 31 | S333 | S | L | W | D | T | S | P | S | Y | I | D | K | L | V | Q | | | | | | | | | | | |
| | Site 32 | Y334 | L | W | D | T | S | P | S | Y | I | D | K | L | V | Q | G | | | | | | | | | | | |
| | Site 33 | S343 | D | K | L | V | Q | G | I | S | F | S | Q | P | T | C | P | | | | | | | | | | | |
| | Site 34 | S345 | L | V | Q | G | I | S | F | S | Q | P | T | C | P | D | H | | | | | | Checkpoint protein-serine kinase 1 S345 phosphosite-specific antibody AB-PK579#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK579#Checkpoint protein-serine kinase 1 (I342-T348, human) pS345 phosphopeptide - Powder PE-04AJU95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AJU95 | | | | | |
| | Site 35 | T348 | G | I | S | F | S | Q | P | T | C | P | D | H | M | L | L | | | | | | | | | | | |
| | Site 36 | S357 | P | D | H | M | L | L | N | S | Q | L | L | G | T | P | G | | | | | | | | | | | |
| | Site 37 | T362 | L | N | S | Q | L | L | G | T | P | G | S | S | Q | N | P | | | | | | | | | | | |
| | Site 38 | S365 | Q | L | L | G | T | P | G | S | S | Q | N | P | W | Q | R | | | | | | | | | | | |
| | Site 39 | S366 | L | L | G | T | P | G | S | S | Q | N | P | W | Q | R | L | | | | | | | | | | | |
| | Site 40 | T378 | Q | R | L | V | K | R | M | T | R | F | F | T | K | L | D | | | | | | | | | | | |
| | Site 41 | T382 | K | R | M | T | R | F | F | T | K | L | D | A | D | K | S | | | | | | | | | | | |
| | Site 42 | S389 | T | K | L | D | A | D | K | S | Y | Q | C | L | K | E | T | | | | | | | | | | | |
| | Site 43 | Y390 | K | L | D | A | D | K | S | Y | Q | C | L | K | E | T | C | | | | | | | | | | | |
| | Site 44 | T396 | S | Y | Q | C | L | K | E | T | C | E | K | L | G | Y | Q | | | | | | | | | | | |
| | Site 45 | Y402 | E | T | C | E | K | L | G | Y | Q | W | K | K | S | C | M | | | | | | | | | | | |
| | Site 46 | T417 | N | Q | V | T | I | S | T | T | D | R | R | N | N | K | L | | | | | | | | | | | |
| | Site 47 | S467 | G | K | L | I | D | I | V | S | S | Q | K | V | W | L | P | | | | | | | | | | | |
| | Site 48 | S468 | K | L | I | D | I | V | S | S | Q | K | V | W | L | P | A | | | | | | | | | | | |
|
|
|
|
|
|
|
|
Legend
|
|
|
|
Confirmed in mammals
|
|
Confirmed in related proteins or other species
|
|
|
Predicted by Kinexus P-Site Prediction algorithm
|
|
|
No data/link available
|
|
|
Link available
|
|
|
Products available
|
|
|
|
|
|
|