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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
EIF2AK4
Full Name:
Eukaryotic translation initiation factor 2-alpha kinase 4
Alias:
E2AK4; EC 2.7.11.1; GCN2-like protein; KIAA1338
Type:
Protein-serine kinase, Other group, PEK family, GCN2 subfamily
Mass (Da):
186838
Number AA:
1649
UniProt ID:
Q9P2K8
International Prot ID:
IPI00163851
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0005622
GO:0005737
GO:0005840
Uniprot
OncoNet
Molecular Function:
GO:0000166
GO:0003824
GO:0004672
PhosphoSite+
KinaseNET
Biological Process:
GO:0006082
GO:0006139
GO:0006399
Phosida
TranscriptoNet
STRING
Kinexus Products
EIF2S1 (46-57) - eIF2-alpha substrate peptide - Powder PE-01ACR95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01ACR95
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S18
G
R
D
E
P
P
E
S
Y
P
Q
R
Q
D
H
Site 2
Y19
R
D
E
P
P
E
S
Y
P
Q
R
Q
D
H
E
Site 3
Y60
P
E
I
N
L
V
L
Y
P
Q
G
L
T
G
E
Site 4
T82
L
R
V
K
C
P
P
T
Y
P
D
V
V
P
E
Site 5
Y83
R
V
K
C
P
P
T
Y
P
D
V
V
P
E
I
Site 6
S99
L
K
N
A
K
G
L
S
N
E
S
V
N
L
L
Site 7
S102
A
K
G
L
S
N
E
S
V
N
L
L
K
S
R
Site 8
S108
E
S
V
N
L
L
K
S
R
L
E
E
L
A
K
Site 9
S132
E
L
A
Y
H
V
Q
S
F
L
S
E
H
N
K
Site 10
S135
Y
H
V
Q
S
F
L
S
E
H
N
K
P
P
P
Site 11
S144
H
N
K
P
P
P
K
S
F
H
E
E
M
L
E
Site 12
S205
Q
E
R
L
E
I
A
S
L
S
N
Q
D
H
T
Site 13
S207
R
L
E
I
A
S
L
S
N
Q
D
H
T
S
K
Site 14
T212
S
L
S
N
Q
D
H
T
S
K
K
D
P
G
G
Site 15
S213
L
S
N
Q
D
H
T
S
K
K
D
P
G
G
H
Site 16
S230
A
A
I
L
H
G
G
S
P
D
F
V
G
N
G
Site 17
S243
N
G
K
H
R
A
N
S
S
G
R
S
R
R
E
Site 18
S244
G
K
H
R
A
N
S
S
G
R
S
R
R
E
R
Site 19
S247
R
A
N
S
S
G
R
S
R
R
E
R
Q
Y
S
Site 20
Y253
R
S
R
R
E
R
Q
Y
S
V
C
N
S
E
D
Site 21
S254
S
R
R
E
R
Q
Y
S
V
C
N
S
E
D
S
Site 22
S258
R
Q
Y
S
V
C
N
S
E
D
S
P
G
S
C
Site 23
S261
S
V
C
N
S
E
D
S
P
G
S
C
E
I
L
Site 24
S264
N
S
E
D
S
P
G
S
C
E
I
L
Y
F
N
Site 25
Y269
P
G
S
C
E
I
L
Y
F
N
M
G
S
P
D
Site 26
S288
H
K
G
K
C
I
G
S
D
E
Q
L
G
K
L
Site 27
Y297
E
Q
L
G
K
L
V
Y
N
A
L
E
T
A
T
Site 28
S327
K
M
G
P
F
L
T
S
Q
E
K
E
K
I
D
Site 29
T345
K
Q
I
Q
G
T
E
T
E
F
N
S
L
V
K
Site 30
S349
G
T
E
T
E
F
N
S
L
V
K
L
S
H
P
Site 31
S354
F
N
S
L
V
K
L
S
H
P
N
V
V
R
Y
Site 32
Y361
S
H
P
N
V
V
R
Y
L
A
M
N
L
K
E
Site 33
S394
L
A
A
H
L
S
H
S
G
P
I
P
V
H
Q
Site 34
Y405
P
V
H
Q
L
R
R
Y
T
A
Q
L
L
S
G
Site 35
T406
V
H
Q
L
R
R
Y
T
A
Q
L
L
S
G
L
Site 36
S411
R
Y
T
A
Q
L
L
S
G
L
D
Y
L
H
S
Site 37
S418
S
G
L
D
Y
L
H
S
N
S
V
V
H
K
V
Site 38
Y444
G
T
V
K
I
T
D
Y
S
I
S
K
R
L
A
Site 39
S445
T
V
K
I
T
D
Y
S
I
S
K
R
L
A
D
Site 40
S447
K
I
T
D
Y
S
I
S
K
R
L
A
D
I
C
Site 41
T462
K
E
D
V
F
E
Q
T
R
V
R
F
S
D
N
Site 42
S467
E
Q
T
R
V
R
F
S
D
N
A
L
P
Y
K
Site 43
Y500
Q
G
Q
E
C
G
E
Y
P
V
T
I
P
S
D
Site 44
T503
E
C
G
E
Y
P
V
T
I
P
S
D
L
P
A
Site 45
S506
E
Y
P
V
T
I
P
S
D
L
P
A
D
F
Q
Site 46
S529
L
D
D
K
E
R
W
S
P
Q
Q
L
L
K
H
Site 47
S537
P
Q
Q
L
L
K
H
S
F
I
N
P
Q
P
K
Site 48
S551
K
M
P
L
V
E
Q
S
P
E
D
S
G
G
Q
Site 49
S555
V
E
Q
S
P
E
D
S
G
G
Q
D
Y
V
E
Site 50
Y560
E
D
S
G
G
Q
D
Y
V
E
T
V
I
P
S
Site 51
T563
G
G
Q
D
Y
V
E
T
V
I
P
S
N
R
L
Site 52
S567
Y
V
E
T
V
I
P
S
N
R
L
P
S
A
A
Site 53
S572
I
P
S
N
R
L
P
S
A
A
F
F
S
E
T
Site 54
T579
S
A
A
F
F
S
E
T
Q
R
Q
F
S
R
Y
Site 55
S584
S
E
T
Q
R
Q
F
S
R
Y
F
I
E
F
E
Site 56
Y586
T
Q
R
Q
F
S
R
Y
F
I
E
F
E
E
L
Site 57
S627
R
I
P
I
N
P
A
S
R
Q
F
R
R
I
K
Site 58
T638
R
R
I
K
G
E
V
T
L
L
S
R
L
H
H
Site 59
S641
K
G
E
V
T
L
L
S
R
L
H
H
E
N
I
Site 60
T667
E
R
P
A
G
P
G
T
P
P
P
D
S
G
P
Site 61
S672
P
G
T
P
P
P
D
S
G
P
L
A
K
D
D
Site 62
S688
A
A
R
G
Q
P
A
S
D
T
D
G
L
D
S
Site 63
T690
R
G
Q
P
A
S
D
T
D
G
L
D
S
V
E
Site 64
S695
S
D
T
D
G
L
D
S
V
E
A
A
A
P
P
Site 65
S706
A
A
P
P
P
I
L
S
S
S
V
E
W
S
T
Site 66
S707
A
P
P
P
I
L
S
S
S
V
E
W
S
T
S
Site 67
S708
P
P
P
I
L
S
S
S
V
E
W
S
T
S
G
Site 68
S712
L
S
S
S
V
E
W
S
T
S
G
E
R
S
A
Site 69
S714
S
S
V
E
W
S
T
S
G
E
R
S
A
S
A
Site 70
S718
W
S
T
S
G
E
R
S
A
S
A
R
F
P
A
Site 71
S720
T
S
G
E
R
S
A
S
A
R
F
P
A
T
G
Site 72
T726
A
S
A
R
F
P
A
T
G
P
G
S
S
D
D
Site 73
S730
F
P
A
T
G
P
G
S
S
D
D
E
D
D
D
Site 74
S746
D
E
H
G
G
V
F
S
Q
S
F
L
P
A
S
Site 75
S748
H
G
G
V
F
S
Q
S
F
L
P
A
S
D
S
Site 76
S753
S
Q
S
F
L
P
A
S
D
S
E
S
D
I
I
Site 77
S755
S
F
L
P
A
S
D
S
E
S
D
I
I
F
D
Site 78
S757
L
P
A
S
D
S
E
S
D
I
I
F
D
N
E
Site 79
S768
F
D
N
E
D
E
N
S
K
S
Q
N
Q
D
E
Site 80
S770
N
E
D
E
N
S
K
S
Q
N
Q
D
E
D
C
Site 81
S786
E
K
N
G
C
H
E
S
E
P
S
V
T
T
E
Site 82
S789
G
C
H
E
S
E
P
S
V
T
T
E
A
V
H
Site 83
T791
H
E
S
E
P
S
V
T
T
E
A
V
H
Y
L
Site 84
Y799
T
E
A
V
H
Y
L
Y
I
Q
M
E
Y
C
E
Site 85
Y804
Y
L
Y
I
Q
M
E
Y
C
E
K
S
T
L
R
Site 86
T813
E
K
S
T
L
R
D
T
I
D
Q
G
L
Y
R
Site 87
T822
D
Q
G
L
Y
R
D
T
V
R
L
W
R
L
F
Site 88
S858
P
V
N
I
F
L
D
S
D
D
H
V
K
I
G
Site 89
S877
A
T
D
H
L
A
F
S
A
D
S
K
Q
D
D
Site 90
T886
D
S
K
Q
D
D
Q
T
G
D
L
I
K
S
D
Site 91
S892
Q
T
G
D
L
I
K
S
D
P
S
G
H
L
T
Site 92
T899
S
D
P
S
G
H
L
T
G
M
V
G
T
A
L
Site 93
S915
V
S
P
E
V
Q
G
S
T
K
S
A
Y
N
Q
Site 94
S918
E
V
Q
G
S
T
K
S
A
Y
N
Q
K
V
D
Site 95
Y920
Q
G
S
T
K
S
A
Y
N
Q
K
V
D
L
F
Site 96
S928
N
Q
K
V
D
L
F
S
L
G
I
I
F
F
E
Site 97
T958
L
N
Q
L
R
D
P
T
S
P
K
F
P
E
D
Site 98
S959
N
Q
L
R
D
P
T
S
P
K
F
P
E
D
F
Site 99
S976
G
E
H
A
K
Q
K
S
V
I
S
W
L
L
N
Site 100
T991
H
D
P
A
K
R
P
T
A
T
E
L
L
K
S
Site 101
S998
T
A
T
E
L
L
K
S
E
L
L
P
P
P
Q
Site 102
T1020
E
V
L
H
H
T
L
T
N
V
D
G
K
A
Y
Site 103
S1040
Q
I
F
S
Q
R
I
S
P
A
I
D
Y
T
Y
Site 104
Y1045
R
I
S
P
A
I
D
Y
T
Y
D
S
D
I
L
Site 105
T1046
I
S
P
A
I
D
Y
T
Y
D
S
D
I
L
K
Site 106
Y1047
S
P
A
I
D
Y
T
Y
D
S
D
I
L
K
G
Site 107
S1057
D
I
L
K
G
N
F
S
I
R
T
A
K
M
Q
Site 108
T1085
H
G
A
V
Q
L
C
T
P
L
L
L
P
R
N
Site 109
Y1096
L
P
R
N
R
Q
I
Y
E
H
N
E
A
A
L
Site 110
Y1137
N
I
L
N
L
K
R
Y
C
I
E
R
V
F
R
Site 111
Y1196
P
A
L
Q
E
R
N
Y
S
I
Y
L
N
H
T
Site 112
Y1199
Q
E
R
N
Y
S
I
Y
L
N
H
T
M
L
L
Site 113
S1248
E
A
K
F
C
N
L
S
L
S
S
N
S
L
C
Site 114
S1253
N
L
S
L
S
S
N
S
L
C
R
L
Y
K
F
Site 115
Y1258
S
N
S
L
C
R
L
Y
K
F
I
E
Q
K
G
Site 116
S1276
D
L
M
P
T
I
N
S
L
I
K
Q
K
T
G
Site 117
T1282
N
S
L
I
K
Q
K
T
G
I
A
Q
L
V
K
Site 118
Y1290
G
I
A
Q
L
V
K
Y
G
L
K
D
L
E
E
Site 119
S1410
S
V
G
Q
M
S
M
S
R
A
I
N
L
T
Q
Site 120
T1416
M
S
R
A
I
N
L
T
Q
K
L
W
T
A
G
Site 121
Y1430
G
I
T
A
E
I
M
Y
D
W
S
Q
S
Q
E
Site 122
S1433
A
E
I
M
Y
D
W
S
Q
S
Q
E
E
L
Q
Site 123
S1435
I
M
Y
D
W
S
Q
S
Q
E
E
L
Q
E
Y
Site 124
Y1442
S
Q
E
E
L
Q
E
Y
C
R
H
H
E
I
T
Site 125
Y1450
C
R
H
H
E
I
T
Y
V
A
L
V
S
D
K
Site 126
S1466
G
S
H
V
K
V
K
S
F
E
K
E
R
Q
T
Site 127
T1473
S
F
E
K
E
R
Q
T
E
K
R
V
L
E
T
Site 128
T1495
Q
K
L
R
T
K
V
T
D
E
R
N
G
R
E
Site 129
S1504
E
R
N
G
R
E
A
S
D
N
L
A
V
Q
N
Site 130
S1515
A
V
Q
N
L
K
G
S
F
S
N
A
S
G
L
Site 131
S1517
Q
N
L
K
G
S
F
S
N
A
S
G
L
F
E
Site 132
S1520
K
G
S
F
S
N
A
S
G
L
F
E
I
H
G
Site 133
S1543
V
L
A
P
E
K
L
S
A
S
T
R
R
R
Y
Site 134
S1545
A
P
E
K
L
S
A
S
T
R
R
R
Y
E
T
Site 135
Y1550
S
A
S
T
R
R
R
Y
E
T
Q
V
Q
T
R
Site 136
T1552
S
T
R
R
R
Y
E
T
Q
V
Q
T
R
L
Q
Site 137
T1560
Q
V
Q
T
R
L
Q
T
S
L
A
N
L
H
Q
Site 138
S1561
V
Q
T
R
L
Q
T
S
L
A
N
L
H
Q
K
Site 139
S1569
L
A
N
L
H
Q
K
S
S
E
I
E
I
L
A
Site 140
S1570
A
N
L
H
Q
K
S
S
E
I
E
I
L
A
V
Site 141
S1608
T
T
V
K
Q
L
L
S
R
L
P
K
Q
R
Y
Site 142
Y1615
S
R
L
P
K
Q
R
Y
L
K
L
V
C
D
E
Site 143
Y1624
K
L
V
C
D
E
I
Y
N
I
K
V
E
K
K
Site 144
Y1638
K
V
S
V
L
F
L
Y
S
Y
R
D
D
Y
Y
Site 145
S1639
V
S
V
L
F
L
Y
S
Y
R
D
D
Y
Y
R
Site 146
Y1640
S
V
L
F
L
Y
S
Y
R
D
D
Y
Y
R
I
Site 147
Y1644
L
Y
S
Y
R
D
D
Y
Y
R
I
L
F
_
_
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation