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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
Wnk4
Full Name:
Serine/threonine-protein kinase WNK4
Alias:
EC 2.7.11.1; Kinase WNK4; PRKWNK4; Protein kinase with no lysine 4; Protein kinase, lysine-deficient 4; WNK lysine deficient protein kinase 4
Type:
EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Other group; Wnk family
Mass (Da):
134739
Number AA:
1243
UniProt ID:
Q96J92
International Prot ID:
IPI00151141
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0005923
Uniprot
OncoNet
Molecular Function:
GO:0005524
GO:0004674
PhosphoSite+
KinaseNET
Biological Process:
GO:0006468
GO:0007243
Phosida
TranscriptoNet
STRING
Kinexus Products
Serine/threonine-protein kinase WNK4 pan-specific antibody AB-NK255-1#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-NK255-1#Serine/threonine-protein kinase WNK4 pan-specific antibody AB-NK255-3#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-NK255-3#Serine/threonine-protein kinase WNK4 (M1-Q15, human) peptide - Powder PE-01AZH85#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01AZH85#Serine/threonine-protein kinase WNK4 (D159-Y173, human) peptide - Powder PE-01AZI99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01AZI99#Serine/threonine-protein kinase WNK4 (R1229-M1243, human) peptide - Powder PE-01AZJ99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01AZJ99
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
T10
A
S
P
A
T
E
T
T
V
L
M
S
Q
T
E
Site 2
S47
P
R
R
A
R
R
F
S
G
K
A
E
P
R
P
Site 3
S56
K
A
E
P
R
P
R
S
S
R
L
S
R
R
S
Site 4
S57
A
E
P
R
P
R
S
S
R
L
S
R
R
S
S
Site 5
S60
R
P
R
S
S
R
L
S
R
R
S
S
V
D
L
Site 6
S63
S
S
R
L
S
R
R
S
S
V
D
L
G
L
L
Site 7
S64
S
R
L
S
R
R
S
S
V
D
L
G
L
L
S
Site 8
S78
S
S
W
S
L
P
A
S
P
A
P
D
P
P
D
Site 9
S89
D
P
P
D
P
P
D
S
A
G
P
G
P
A
R
Site 10
S97
A
G
P
G
P
A
R
S
P
P
P
S
S
K
E
Site 11
S101
P
A
R
S
P
P
P
S
S
K
E
P
P
E
G
Site 12
S102
A
R
S
P
P
P
S
S
K
E
P
P
E
G
T
Site 13
T111
E
P
P
E
G
T
W
T
E
G
A
P
V
K
A
Site 14
S122
P
V
K
A
A
E
D
S
A
R
P
E
L
P
D
Site 15
S130
A
R
P
E
L
P
D
S
A
V
G
P
G
S
R
Site 16
S136
D
S
A
V
G
P
G
S
R
E
P
L
R
V
P
Site 17
T162
E
E
K
E
D
M
E
T
Q
A
V
A
T
S
P
Site 18
S168
E
T
Q
A
V
A
T
S
P
D
G
R
Y
L
K
Site 19
Y173
A
T
S
P
D
G
R
Y
L
K
F
D
I
E
I
Site 20
S184
D
I
E
I
G
R
G
S
F
K
T
V
Y
R
G
Site 21
T187
I
G
R
G
S
F
K
T
V
Y
R
G
L
D
T
Site 22
Y189
R
G
S
F
K
T
V
Y
R
G
L
D
T
D
T
Site 23
T196
Y
R
G
L
D
T
D
T
T
V
E
V
A
W
C
Site 24
T197
R
G
L
D
T
D
T
T
V
E
V
A
W
C
E
Site 25
S211
E
L
Q
T
R
K
L
S
R
A
E
R
Q
R
F
Site 26
S219
R
A
E
R
Q
R
F
S
E
E
V
E
M
L
K
Site 27
Y237
H
P
N
I
V
R
F
Y
D
S
W
K
S
V
L
Site 28
S239
N
I
V
R
F
Y
D
S
W
K
S
V
L
R
G
Site 29
T261
T
E
L
M
T
S
G
T
L
K
T
Y
L
R
R
Site 30
Y265
T
S
G
T
L
K
T
Y
L
R
R
F
R
E
M
Site 31
S281
P
R
V
L
Q
R
W
S
R
Q
I
L
R
G
L
Site 32
S293
R
G
L
H
F
L
H
S
R
V
P
P
I
L
H
Site 33
T326
I
G
D
L
G
L
A
T
L
K
R
A
S
F
A
Site 34
S331
L
A
T
L
K
R
A
S
F
A
K
S
V
I
G
Site 35
S335
K
R
A
S
F
A
K
S
V
I
G
T
P
E
F
Site 36
Y352
P
E
M
Y
E
E
K
Y
D
E
A
V
D
V
Y
Site 37
Y373
L
E
M
A
T
S
E
Y
P
Y
S
E
C
Q
N
Site 38
S376
A
T
S
E
Y
P
Y
S
E
C
Q
N
A
A
Q
Site 39
T389
A
Q
I
Y
R
K
V
T
S
G
R
K
P
N
S
Site 40
S390
Q
I
Y
R
K
V
T
S
G
R
K
P
N
S
F
Site 41
S396
T
S
G
R
K
P
N
S
F
H
K
V
K
I
P
Site 42
T422
T
D
K
N
E
R
F
T
I
Q
D
L
L
A
H
Site 43
T543
E
P
G
P
P
P
A
T
V
P
M
A
P
G
P
Site 44
S552
P
M
A
P
G
P
P
S
V
F
P
P
E
P
E
Site 45
S575
P
F
L
F
R
H
A
S
Y
S
S
T
T
S
D
Site 46
S577
L
F
R
H
A
S
Y
S
S
T
T
S
D
C
E
Site 47
T579
R
H
A
S
Y
S
S
T
T
S
D
C
E
T
D
Site 48
S581
A
S
Y
S
S
T
T
S
D
C
E
T
D
G
Y
Site 49
T585
S
T
T
S
D
C
E
T
D
G
Y
L
S
S
S
Site 50
Y588
S
D
C
E
T
D
G
Y
L
S
S
S
G
F
L
Site 51
S590
C
E
T
D
G
Y
L
S
S
S
G
F
L
D
A
Site 52
S591
E
T
D
G
Y
L
S
S
S
G
F
L
D
A
S
Site 53
S592
T
D
G
Y
L
S
S
S
G
F
L
D
A
S
D
Site 54
S598
S
S
G
F
L
D
A
S
D
P
A
L
Q
P
P
Site 55
S610
Q
P
P
G
G
V
P
S
S
L
A
E
S
H
L
Site 56
S611
P
P
G
G
V
P
S
S
L
A
E
S
H
L
C
Site 57
S630
F
A
L
S
I
P
R
S
G
P
G
S
D
F
S
Site 58
S634
I
P
R
S
G
P
G
S
D
F
S
P
G
D
S
Site 59
S637
S
G
P
G
S
D
F
S
P
G
D
S
Y
A
S
Site 60
S641
S
D
F
S
P
G
D
S
Y
A
S
D
A
A
S
Site 61
Y642
D
F
S
P
G
D
S
Y
A
S
D
A
A
S
G
Site 62
S644
S
P
G
D
S
Y
A
S
D
A
A
S
G
L
S
Site 63
S648
S
Y
A
S
D
A
A
S
G
L
S
D
V
G
E
Site 64
S651
S
D
A
A
S
G
L
S
D
V
G
E
G
M
G
Site 65
S674
N
L
R
R
R
P
R
S
R
L
R
V
T
S
V
Site 66
T679
P
R
S
R
L
R
V
T
S
V
S
D
Q
N
D
Site 67
S680
R
S
R
L
R
V
T
S
V
S
D
Q
N
D
R
Site 68
S682
R
L
R
V
T
S
V
S
D
Q
N
D
R
V
V
Site 69
T702
T
H
N
S
K
M
V
T
F
R
F
D
L
D
G
Site 70
S711
R
F
D
L
D
G
D
S
P
E
E
I
A
A
A
Site 71
S728
Y
N
E
F
I
L
P
S
E
R
D
G
F
L
R
Site 72
T746
E
I
I
Q
R
V
E
T
L
L
K
R
D
T
G
Site 73
T752
E
T
L
L
K
R
D
T
G
P
M
E
A
A
E
Site 74
T761
P
M
E
A
A
E
D
T
L
S
P
Q
E
E
P
Site 75
S763
E
A
A
E
D
T
L
S
P
Q
E
E
P
A
P
Site 76
S782
P
V
P
L
P
D
P
S
N
E
E
L
Q
S
S
Site 77
S788
P
S
N
E
E
L
Q
S
S
T
S
L
E
H
R
Site 78
S789
S
N
E
E
L
Q
S
S
T
S
L
E
H
R
S
Site 79
S791
E
E
L
Q
S
S
T
S
L
E
H
R
S
W
T
Site 80
S796
S
T
S
L
E
H
R
S
W
T
A
F
S
T
S
Site 81
T798
S
L
E
H
R
S
W
T
A
F
S
T
S
S
S
Site 82
S801
H
R
S
W
T
A
F
S
T
S
S
S
S
P
G
Site 83
T802
R
S
W
T
A
F
S
T
S
S
S
S
P
G
T
Site 84
S803
S
W
T
A
F
S
T
S
S
S
S
P
G
T
P
Site 85
S804
W
T
A
F
S
T
S
S
S
S
P
G
T
P
L
Site 86
S805
T
A
F
S
T
S
S
S
S
P
G
T
P
L
S
Site 87
S806
A
F
S
T
S
S
S
S
P
G
T
P
L
S
P
Site 88
T809
T
S
S
S
S
P
G
T
P
L
S
P
G
N
P
Site 89
S812
S
S
P
G
T
P
L
S
P
G
N
P
F
S
P
Site 90
S818
L
S
P
G
N
P
F
S
P
G
T
P
I
S
P
Site 91
T821
G
N
P
F
S
P
G
T
P
I
S
P
G
P
I
Site 92
T832
P
G
P
I
F
P
I
T
S
P
P
C
H
P
S
Site 93
S833
G
P
I
F
P
I
T
S
P
P
C
H
P
S
P
Site 94
S839
T
S
P
P
C
H
P
S
P
S
P
F
S
P
I
Site 95
S841
P
P
C
H
P
S
P
S
P
F
S
P
I
S
S
Site 96
S844
H
P
S
P
S
P
F
S
P
I
S
S
Q
V
S
Site 97
S847
P
S
P
F
S
P
I
S
S
Q
V
S
S
N
P
Site 98
S848
S
P
F
S
P
I
S
S
Q
V
S
S
N
P
S
Site 99
S851
S
P
I
S
S
Q
V
S
S
N
P
S
P
H
P
Site 100
S852
P
I
S
S
Q
V
S
S
N
P
S
P
H
P
T
Site 101
S855
S
Q
V
S
S
N
P
S
P
H
P
T
S
S
P
Site 102
T859
S
N
P
S
P
H
P
T
S
S
P
L
P
F
S
Site 103
S860
N
P
S
P
H
P
T
S
S
P
L
P
F
S
S
Site 104
S861
P
S
P
H
P
T
S
S
P
L
P
F
S
S
S
Site 105
S866
T
S
S
P
L
P
F
S
S
S
T
P
E
F
P
Site 106
S867
S
S
P
L
P
F
S
S
S
T
P
E
F
P
V
Site 107
S868
S
P
L
P
F
S
S
S
T
P
E
F
P
V
P
Site 108
T869
P
L
P
F
S
S
S
T
P
E
F
P
V
P
L
Site 109
S877
P
E
F
P
V
P
L
S
Q
C
P
W
S
S
L
Site 110
S882
P
L
S
Q
C
P
W
S
S
L
P
T
T
S
P
Site 111
S883
L
S
Q
C
P
W
S
S
L
P
T
T
S
P
P
Site 112
T886
C
P
W
S
S
L
P
T
T
S
P
P
T
F
S
Site 113
T887
P
W
S
S
L
P
T
T
S
P
P
T
F
S
P
Site 114
S888
W
S
S
L
P
T
T
S
P
P
T
F
S
P
T
Site 115
T891
L
P
T
T
S
P
P
T
F
S
P
T
C
S
Q
Site 116
S893
T
T
S
P
P
T
F
S
P
T
C
S
Q
V
T
Site 117
T895
S
P
P
T
F
S
P
T
C
S
Q
V
T
L
S
Site 118
S897
P
T
F
S
P
T
C
S
Q
V
T
L
S
S
P
Site 119
T900
S
P
T
C
S
Q
V
T
L
S
S
P
F
F
P
Site 120
S911
P
F
F
P
P
C
P
S
T
S
S
F
P
S
T
Site 121
T912
F
F
P
P
C
P
S
T
S
S
F
P
S
T
T
Site 122
S913
F
P
P
C
P
S
T
S
S
F
P
S
T
T
A
Site 123
S914
P
P
C
P
S
T
S
S
F
P
S
T
T
A
A
Site 124
S945
A
Q
S
L
L
S
P
S
P
G
L
L
S
Q
S
Site 125
S950
S
P
S
P
G
L
L
S
Q
S
P
P
A
P
P
Site 126
S952
S
P
G
L
L
S
Q
S
P
P
A
P
P
S
P
Site 127
S958
Q
S
P
P
A
P
P
S
P
L
P
S
L
P
L
Site 128
S976
V
A
P
G
G
Q
E
S
P
S
P
H
T
A
E
Site 129
S978
P
G
G
Q
E
S
P
S
P
H
T
A
E
V
E
Site 130
S986
P
H
T
A
E
V
E
S
E
A
S
P
P
P
A
Site 131
S989
A
E
V
E
S
E
A
S
P
P
P
A
R
P
L
Site 132
S1006
E
A
R
L
A
P
I
S
E
E
G
K
P
Q
L
Site 133
S1021
V
G
R
F
Q
V
T
S
S
K
E
P
A
E
P
Site 134
T1034
E
P
L
P
L
Q
P
T
S
P
T
L
S
G
S
Site 135
S1035
P
L
P
L
Q
P
T
S
P
T
L
S
G
S
P
Site 136
T1037
P
L
Q
P
T
S
P
T
L
S
G
S
P
K
P
Site 137
S1039
Q
P
T
S
P
T
L
S
G
S
P
K
P
S
T
Site 138
S1041
T
S
P
T
L
S
G
S
P
K
P
S
T
P
Q
Site 139
S1045
L
S
G
S
P
K
P
S
T
P
Q
L
T
S
E
Site 140
T1046
S
G
S
P
K
P
S
T
P
Q
L
T
S
E
S
Site 141
T1050
K
P
S
T
P
Q
L
T
S
E
S
S
D
T
E
Site 142
S1051
P
S
T
P
Q
L
T
S
E
S
S
D
T
E
D
Site 143
S1054
P
Q
L
T
S
E
S
S
D
T
E
D
S
A
G
Site 144
T1056
L
T
S
E
S
S
D
T
E
D
S
A
G
G
G
Site 145
S1059
E
S
S
D
T
E
D
S
A
G
G
G
P
E
T
Site 146
S1073
T
R
E
A
L
A
E
S
D
R
A
A
E
G
L
Site 147
S1099
K
E
P
Q
V
G
G
S
P
Q
P
L
S
H
P
Site 148
S1104
G
G
S
P
Q
P
L
S
H
P
S
P
V
W
M
Site 149
S1107
P
Q
P
L
S
H
P
S
P
V
W
M
N
Y
S
Site 150
Y1113
P
S
P
V
W
M
N
Y
S
Y
S
S
L
C
L
Site 151
S1114
S
P
V
W
M
N
Y
S
Y
S
S
L
C
L
S
Site 152
Y1115
P
V
W
M
N
Y
S
Y
S
S
L
C
L
S
S
Site 153
S1116
V
W
M
N
Y
S
Y
S
S
L
C
L
S
S
E
Site 154
S1117
W
M
N
Y
S
Y
S
S
L
C
L
S
S
E
E
Site 155
S1121
S
Y
S
S
L
C
L
S
S
E
E
S
E
S
S
Site 156
S1122
Y
S
S
L
C
L
S
S
E
E
S
E
S
S
G
Site 157
S1125
L
C
L
S
S
E
E
S
E
S
S
G
E
D
E
Site 158
S1127
L
S
S
E
E
S
E
S
S
G
E
D
E
E
F
Site 159
S1128
S
S
E
E
S
E
S
S
G
E
D
E
E
F
W
Site 160
S1140
E
F
W
A
E
L
Q
S
L
R
Q
K
H
L
S
Site 161
S1147
S
L
R
Q
K
H
L
S
E
V
E
T
L
Q
T
Site 162
T1151
K
H
L
S
E
V
E
T
L
Q
T
L
Q
K
K
Site 163
T1154
S
E
V
E
T
L
Q
T
L
Q
K
K
E
I
E
Site 164
Y1164
K
K
E
I
E
D
L
Y
S
R
L
G
K
Q
P
Site 165
S1165
K
E
I
E
D
L
Y
S
R
L
G
K
Q
P
P
Site 166
S1183
V
A
P
A
A
M
L
S
S
R
Q
R
R
L
S
Site 167
S1184
A
P
A
A
M
L
S
S
R
Q
R
R
L
S
K
Site 168
S1190
S
S
R
Q
R
R
L
S
K
G
S
F
P
T
S
Site 169
S1193
Q
R
R
L
S
K
G
S
F
P
T
S
R
R
N
Site 170
T1196
L
S
K
G
S
F
P
T
S
R
R
N
S
L
Q
Site 171
S1197
S
K
G
S
F
P
T
S
R
R
N
S
L
Q
R
Site 172
S1201
F
P
T
S
R
R
N
S
L
Q
R
S
E
P
P
Site 173
S1205
R
R
N
S
L
Q
R
S
E
P
P
G
P
G
I
Site 174
S1217
P
G
I
M
R
R
N
S
L
S
G
S
S
T
G
Site 175
S1219
I
M
R
R
N
S
L
S
G
S
S
T
G
S
Q
Site 176
S1221
R
R
N
S
L
S
G
S
S
T
G
S
Q
E
Q
Site 177
T1223
N
S
L
S
G
S
S
T
G
S
Q
E
Q
R
A
Site 178
S1225
L
S
G
S
S
T
G
S
Q
E
Q
R
A
S
K
Site 179
S1231
G
S
Q
E
Q
R
A
S
K
G
V
T
F
A
G
Site 180
T1235
Q
R
A
S
K
G
V
T
F
A
G
D
V
G
R
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation