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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
MLK3
Full Name:
Mitogen-activated protein kinase kinase kinase 11
Alias:
EC 2.7.11.25; Kinase MLK3; M3K11; MAP3K11; MAPKKK11; MEKK11; Mitogen activated protein kinase kinase kinase 11; Mixed lineage kinase 3; MLK-3; PTK1; Similar to mitogen-activated protein kinase kinase kinase 11; SPRK
Type:
Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.25;
TKL
group; MLK family; MLK subfamily
Mass (Da):
92688
Number AA:
847
UniProt ID:
Q16584
International Prot ID:
IPI00000977
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0005813
GO:0005874
Uniprot
OncoNet
Molecular Function:
GO:0005524
GO:0004706
GO:0042803
PhosphoSite+
KinaseNET
Biological Process:
GO:0000080
GO:0007257
GO:0008283
Phosida
TranscriptoNet
STRING
Kinexus Products
Mixed-lineage protein-serine kinase 3; Mitogen-activated protein kinase kinase kinase 11 S281 phosphosite-specific antibody AB-PK718#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK718#Mixed-lineage protein-serine kinase 3; Mitogen-activated protein kinase kinase kinase 11 T277+S281 phosphosite-specific antibody AB-PK870#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK870#Mixed-lineage protein-serine kinase 3 / Mitogen-activated protein kinase kinase kinase 11 (T278-G284, human) pS281 phosphopeptide - Powder PE-04AOB85#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AOB85#Mixed-lineage protein-serine kinase 3 / Mitogen-activated protein kinase kinase kinase 11 (W274-G280, human) pT277+pS281 phosphopeptide - Powder PE-04AQV65#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AQV65
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S11
L
K
S
L
F
L
K
S
P
L
G
S
W
N
G
Site 2
S15
F
L
K
S
P
L
G
S
W
N
G
S
G
S
G
Site 3
S19
P
L
G
S
W
N
G
S
G
S
G
G
G
G
G
Site 4
S21
G
S
W
N
G
S
G
S
G
G
G
G
G
G
G
Site 5
S35
G
G
G
R
P
E
G
S
P
K
A
A
G
Y
A
Site 6
Y41
G
S
P
K
A
A
G
Y
A
N
P
V
W
T
A
Site 7
Y52
V
W
T
A
L
F
D
Y
E
P
S
G
Q
D
E
Site 8
S72
G
D
R
V
E
V
L
S
R
D
A
A
I
S
G
Site 9
S78
L
S
R
D
A
A
I
S
G
D
E
G
W
W
A
Site 10
S97
G
Q
V
G
I
F
P
S
N
Y
V
S
R
G
G
Site 11
Y99
V
G
I
F
P
S
N
Y
V
S
R
G
G
G
P
Site 12
S101
I
F
P
S
N
Y
V
S
R
G
G
G
P
P
P
Site 13
S113
P
P
P
C
E
V
A
S
F
Q
E
L
R
L
E
Site 14
Y132
I
G
G
F
G
K
V
Y
R
G
S
W
R
G
E
Site 15
S135
F
G
K
V
Y
R
G
S
W
R
G
E
L
V
A
Site 16
S155
Q
D
P
D
E
D
I
S
V
T
A
E
S
V
R
Site 17
S160
D
I
S
V
T
A
E
S
V
R
Q
E
A
R
L
Site 18
S202
Y
A
A
G
G
P
L
S
R
A
L
A
G
R
R
Site 19
S255
L
L
L
Q
P
I
E
S
D
D
M
E
H
K
T
Site 20
T262
S
D
D
M
E
H
K
T
L
K
I
T
D
F
G
Site 21
T277
L
A
R
E
W
H
K
T
T
Q
M
S
A
A
G
Mixed-lineage protein-serine kinase 3; Mitogen-activated protein kinase kinase kinase 11 T277+S281 phosphosite-specific antibody AB-PK870#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK870#Mixed-lineage protein-serine kinase 3 / Mitogen-activated protein kinase kinase kinase 11 (W274-G280, human) pT277+pS281 phosphopeptide - Powder PE-04AQV65#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AQV65
Site 22
S281
W
H
K
T
T
Q
M
S
A
A
G
T
Y
A
W
Mixed-lineage protein-serine kinase 3; Mitogen-activated protein kinase kinase kinase 11 S281 phosphosite-specific antibody AB-PK718#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK718#Mixed-lineage protein-serine kinase 3; Mitogen-activated protein kinase kinase kinase 11 T277+S281 phosphosite-specific antibody AB-PK870#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK870#Mixed-lineage protein-serine kinase 3 / Mitogen-activated protein kinase kinase kinase 11 (T278-G284, human) pS281 phosphopeptide - Powder PE-04AOB85#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AOB85#Mixed-lineage protein-serine kinase 3 / Mitogen-activated protein kinase kinase kinase 11 (W274-G280, human) pT277+pS281 phosphopeptide - Powder PE-04AQV65#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AQV65
Site 23
T285
T
Q
M
S
A
A
G
T
Y
A
W
M
A
P
E
Site 24
S297
A
P
E
V
I
K
A
S
T
F
S
K
G
S
D
Site 25
T298
P
E
V
I
K
A
S
T
F
S
K
G
S
D
V
Site 26
S303
A
S
T
F
S
K
G
S
D
V
W
S
F
G
V
Site 27
S307
S
K
G
S
D
V
W
S
F
G
V
L
L
W
E
Site 28
S371
H
R
R
P
D
F
A
S
I
L
Q
Q
L
E
A
Site 29
S391
L
R
E
M
P
R
D
S
F
H
S
M
Q
E
G
Site 30
S394
M
P
R
D
S
F
H
S
M
Q
E
G
W
K
R
Site 31
S419
A
K
E
K
E
L
L
S
R
E
E
E
L
T
R
Site 32
T425
L
S
R
E
E
E
L
T
R
A
A
R
E
Q
R
Site 33
S433
R
A
A
R
E
Q
R
S
Q
A
E
Q
L
R
R
Site 34
T458
E
V
F
E
R
E
L
T
L
L
L
Q
Q
V
D
Site 35
T477
H
V
R
R
R
R
G
T
F
K
R
S
K
L
R
Site 36
S481
R
R
G
T
F
K
R
S
K
L
R
A
R
D
G
Site 37
S493
R
D
G
G
E
R
I
S
M
P
L
D
F
K
H
Site 38
T503
L
D
F
K
H
R
I
T
V
Q
A
S
P
G
L
Site 39
S507
H
R
I
T
V
Q
A
S
P
G
L
D
R
R
R
Site 40
S524
F
E
V
G
P
G
D
S
P
T
F
P
R
F
R
Site 41
T526
V
G
P
G
D
S
P
T
F
P
R
F
R
A
I
Site 42
S548
G
Q
A
W
G
R
Q
S
P
R
R
L
E
D
S
Site 43
S555
S
P
R
R
L
E
D
S
S
N
G
E
R
R
A
Site 44
S556
P
R
R
L
E
D
S
S
N
G
E
R
R
A
C
Site 45
S569
A
C
W
A
W
G
P
S
S
P
K
P
G
E
A
Site 46
S570
C
W
A
W
G
P
S
S
P
K
P
G
E
A
Q
Site 47
S583
A
Q
N
G
R
R
R
S
R
M
D
E
A
T
W
Site 48
T589
R
S
R
M
D
E
A
T
W
Y
L
D
S
D
D
Site 49
Y591
R
M
D
E
A
T
W
Y
L
D
S
D
D
S
S
Site 50
S594
E
A
T
W
Y
L
D
S
D
D
S
S
P
L
G
Site 51
S597
W
Y
L
D
S
D
D
S
S
P
L
G
S
P
S
Site 52
S598
Y
L
D
S
D
D
S
S
P
L
G
S
P
S
T
Site 53
S602
D
D
S
S
P
L
G
S
P
S
T
P
P
A
L
Site 54
S604
S
S
P
L
G
S
P
S
T
P
P
A
L
N
G
Site 55
T605
S
P
L
G
S
P
S
T
P
P
A
L
N
G
N
Site 56
S617
N
G
N
P
P
R
P
S
L
E
P
E
E
P
K
Site 57
S633
P
V
P
A
E
R
G
S
S
S
G
T
P
K
L
Site 58
S634
V
P
A
E
R
G
S
S
S
G
T
P
K
L
I
Site 59
S635
P
A
E
R
G
S
S
S
G
T
P
K
L
I
Q
Site 60
T637
E
R
G
S
S
S
G
T
P
K
L
I
Q
R
A
Site 61
S654
R
G
T
A
L
L
A
S
L
G
L
G
R
D
L
Site 62
S674
P
G
R
E
R
G
E
S
P
T
T
P
P
T
P
Site 63
T676
R
E
R
G
E
S
P
T
T
P
P
T
P
T
P
Site 64
T677
E
R
G
E
S
P
T
T
P
P
T
P
T
P
A
Site 65
T680
E
S
P
T
T
P
P
T
P
T
P
A
P
C
P
Site 66
T682
P
T
T
P
P
T
P
T
P
A
P
C
P
T
E
Site 67
T688
P
T
P
A
P
C
P
T
E
P
P
P
S
P
L
Site 68
S693
C
P
T
E
P
P
P
S
P
L
I
C
F
S
L
Site 69
S699
P
S
P
L
I
C
F
S
L
K
T
P
D
S
P
Site 70
T702
L
I
C
F
S
L
K
T
P
D
S
P
P
T
P
Site 71
S705
F
S
L
K
T
P
D
S
P
P
T
P
A
P
L
Site 72
T708
K
T
P
D
S
P
P
T
P
A
P
L
L
L
D
Site 73
S724
G
I
P
V
G
Q
R
S
A
K
S
P
R
R
E
Site 74
S727
V
G
Q
R
S
A
K
S
P
R
R
E
E
E
P
Site 75
T738
E
E
E
P
R
G
G
T
V
S
P
P
P
G
T
Site 76
S740
E
P
R
G
G
T
V
S
P
P
P
G
T
S
R
Site 77
T745
T
V
S
P
P
P
G
T
S
R
S
A
P
G
T
Site 78
S746
V
S
P
P
P
G
T
S
R
S
A
P
G
T
P
Site 79
S748
P
P
P
G
T
S
R
S
A
P
G
T
P
G
T
Site 80
T752
T
S
R
S
A
P
G
T
P
G
T
P
R
S
P
Site 81
T755
S
A
P
G
T
P
G
T
P
R
S
P
P
L
G
Site 82
S758
G
T
P
G
T
P
R
S
P
P
L
G
L
I
S
Site 83
S765
S
P
P
L
G
L
I
S
R
P
R
P
S
P
L
Site 84
S770
L
I
S
R
P
R
P
S
P
L
R
S
R
I
D
Site 85
S774
P
R
P
S
P
L
R
S
R
I
D
P
W
S
F
Site 86
S780
R
S
R
I
D
P
W
S
F
V
S
A
G
P
R
Site 87
S783
I
D
P
W
S
F
V
S
A
G
P
R
P
S
P
Site 88
S789
V
S
A
G
P
R
P
S
P
L
P
S
P
Q
P
Site 89
S793
P
R
P
S
P
L
P
S
P
Q
P
A
P
R
R
Site 90
T804
A
P
R
R
A
P
W
T
L
F
P
D
S
D
P
Site 91
S809
P
W
T
L
F
P
D
S
D
P
F
W
D
S
P
Site 92
S815
D
S
D
P
F
W
D
S
P
P
A
N
P
F
Q
Site 93
T832
P
Q
D
C
R
A
Q
T
K
D
M
G
A
Q
A
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation