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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
TRIM28
Full Name:
Transcription intermediary factor 1-beta
Alias:
KAP-1; KRIP-1; RING finger protein 96; TIF1-beta; Tripartite motif-containing protein 28
Type:
Protein kinase, atypical; Nuclear receptor co-regulator; Transcription, coactivator/corepressor;
ATYPICAL
group; TIF1 family
Mass (Da):
88550
Number AA:
835
UniProt ID:
Q13263
International Prot ID:
IPI00438229
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0005622
GO:0005634
GO:0043226
Uniprot
OncoNet
Molecular Function:
GO:0003676
GO:0003677
GO:0003700
PhosphoSite+
KinaseNET
Biological Process:
GO:0000902
GO:0000904
GO:0001837
Phosida
TranscriptoNet
STRING
Kinexus Products
Transcription intermediary factor 1-beta Y458 phosphosite-specific antibody AB-PK834#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK834#Transcription intermediary factor 1-beta Y517 phosphosite-specific antibody AB-PK835#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK835#Transcription intermediary factor 1-beta (T514-I520, human) pY517 phosphopeptide - Powder PE-04APY99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04APY99#Transcription intermediary factor 1-beta (D455-A461, human) pY458 phosphopeptide - Powder PE-04APZ99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04APZ99
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S4
_
_
_
_
M
A
A
S
A
A
A
A
S
A
A
Site 2
S9
A
A
S
A
A
A
A
S
A
A
A
A
S
A
A
Site 3
S14
A
A
S
A
A
A
A
S
A
A
S
G
S
P
G
Site 4
S17
A
A
A
A
S
A
A
S
G
S
P
G
P
G
E
Site 5
S19
A
A
S
A
A
S
G
S
P
G
P
G
E
G
S
Site 6
S26
S
P
G
P
G
E
G
S
A
G
G
E
K
R
S
Site 7
S33
S
A
G
G
E
K
R
S
T
A
P
S
A
A
A
Site 8
T34
A
G
G
E
K
R
S
T
A
P
S
A
A
A
S
Site 9
S37
E
K
R
S
T
A
P
S
A
A
A
S
A
S
A
Site 10
S41
T
A
P
S
A
A
A
S
A
S
A
S
A
A
A
Site 11
S45
A
A
A
S
A
S
A
S
A
A
A
S
S
P
A
Site 12
S49
A
S
A
S
A
A
A
S
S
P
A
G
G
G
A
Site 13
S50
S
A
S
A
A
A
S
S
P
A
G
G
G
A
E
Site 14
S103
A
P
A
A
A
N
S
S
G
D
G
G
A
A
G
Site 15
Y133
S
K
D
I
V
E
N
Y
F
M
R
D
S
G
S
Site 16
S138
E
N
Y
F
M
R
D
S
G
S
K
A
A
T
D
Site 17
S140
Y
F
M
R
D
S
G
S
K
A
A
T
D
A
Q
Site 18
T144
D
S
G
S
K
A
A
T
D
A
Q
D
A
N
Q
Site 19
Y186
E
A
H
Q
R
V
K
Y
T
K
D
H
T
V
R
Site 20
T191
V
K
Y
T
K
D
H
T
V
R
S
T
G
P
A
Site 21
S194
T
K
D
H
T
V
R
S
T
G
P
A
K
S
R
Site 22
T195
K
D
H
T
V
R
S
T
G
P
A
K
S
R
D
Site 23
S200
R
S
T
G
P
A
K
S
R
D
G
E
R
T
V
Site 24
T206
K
S
R
D
G
E
R
T
V
Y
C
N
V
H
K
Site 25
Y208
R
D
G
E
R
T
V
Y
C
N
V
H
K
H
E
Site 26
Y242
N
A
H
K
D
H
Q
Y
Q
F
L
E
D
A
V
Site 27
S258
N
Q
R
K
L
L
A
S
L
V
K
R
L
G
D
Site 28
T269
R
L
G
D
K
H
A
T
L
Q
K
S
T
K
E
Site 29
S279
K
S
T
K
E
V
R
S
S
I
R
Q
V
S
D
Site 30
S280
S
T
K
E
V
R
S
S
I
R
Q
V
S
D
V
Site 31
S285
R
S
S
I
R
Q
V
S
D
V
Q
K
R
V
Q
Site 32
S350
E
H
I
L
R
F
A
S
W
A
L
E
S
D
N
Site 33
T359
A
L
E
S
D
N
N
T
A
L
L
L
S
K
K
Site 34
Y369
L
L
S
K
K
L
I
Y
F
Q
L
H
R
A
L
Site 35
S401
D
L
N
A
W
T
K
S
A
E
A
F
G
K
I
Site 36
T415
I
V
A
E
R
P
G
T
N
S
T
G
P
A
P
Site 37
S417
A
E
R
P
G
T
N
S
T
G
P
A
P
M
A
Site 38
S433
P
R
A
P
G
P
L
S
K
Q
G
S
G
S
S
Site 39
S437
G
P
L
S
K
Q
G
S
G
S
S
Q
P
M
E
Site 40
S439
L
S
K
Q
G
S
G
S
S
Q
P
M
E
V
Q
Site 41
S440
S
K
Q
G
S
G
S
S
Q
P
M
E
V
Q
E
Site 42
Y449
P
M
E
V
Q
E
G
Y
G
F
G
S
G
D
D
Site 43
S453
Q
E
G
Y
G
F
G
S
G
D
D
P
Y
S
S
Site 44
Y458
F
G
S
G
D
D
P
Y
S
S
A
E
P
H
V
Transcription intermediary factor 1-beta Y458 phosphosite-specific antibody AB-PK834#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK834#Transcription intermediary factor 1-beta (D455-A461, human) pY458 phosphopeptide - Powder PE-04APZ99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04APZ99
Site 45
S459
G
S
G
D
D
P
Y
S
S
A
E
P
H
V
S
Site 46
S460
S
G
D
D
P
Y
S
S
A
E
P
H
V
S
G
Site 47
S466
S
S
A
E
P
H
V
S
G
V
K
R
S
R
S
Site 48
S471
H
V
S
G
V
K
R
S
R
S
G
E
G
E
V
Site 49
S473
S
G
V
K
R
S
R
S
G
E
G
E
V
S
G
Site 50
S479
R
S
G
E
G
E
V
S
G
L
M
R
K
V
P
Site 51
S489
M
R
K
V
P
R
V
S
L
E
R
L
D
L
D
Site 52
T498
E
R
L
D
L
D
L
T
A
D
S
Q
P
P
V
Site 53
S501
D
L
D
L
T
A
D
S
Q
P
P
V
F
K
V
Site 54
S512
V
F
K
V
F
P
G
S
T
T
E
D
Y
N
L
Site 55
T514
K
V
F
P
G
S
T
T
E
D
Y
N
L
I
V
Site 56
Y517
P
G
S
T
T
E
D
Y
N
L
I
V
I
E
R
Transcription intermediary factor 1-beta Y517 phosphosite-specific antibody AB-PK835#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK835#Transcription intermediary factor 1-beta (T514-I520, human) pY517 phosphopeptide - Powder PE-04APY99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04APY99
Site 57
T531
R
G
A
A
A
A
A
T
G
Q
P
G
T
A
P
Site 58
T536
A
A
T
G
Q
P
G
T
A
P
A
G
T
P
G
Site 59
T541
P
G
T
A
P
A
G
T
P
G
A
P
P
L
A
Site 60
T567
A
A
I
G
A
P
P
T
A
T
E
G
P
E
T
Site 61
T569
I
G
A
P
P
T
A
T
E
G
P
E
T
K
P
Site 62
S594
A
E
G
P
R
L
A
S
P
S
G
S
T
S
S
Site 63
S596
G
P
R
L
A
S
P
S
G
S
T
S
S
G
L
Site 64
S598
R
L
A
S
P
S
G
S
T
S
S
G
L
E
V
Site 65
T599
L
A
S
P
S
G
S
T
S
S
G
L
E
V
V
Site 66
S600
A
S
P
S
G
S
T
S
S
G
L
E
V
V
A
Site 67
S601
S
P
S
G
S
T
S
S
G
L
E
V
V
A
P
Site 68
T611
E
V
V
A
P
E
G
T
S
A
P
G
G
G
P
Site 69
S612
V
V
A
P
E
G
T
S
A
P
G
G
G
P
G
Site 70
T620
A
P
G
G
G
P
G
T
L
D
D
S
A
T
I
Site 71
S624
G
P
G
T
L
D
D
S
A
T
I
C
R
V
C
Site 72
T626
G
T
L
D
D
S
A
T
I
C
R
V
C
Q
K
Site 73
S667
P
G
E
E
W
S
C
S
L
C
H
V
L
P
D
Site 74
S681
D
L
K
E
E
D
G
S
L
S
L
D
G
A
D
Site 75
S683
K
E
E
D
G
S
L
S
L
D
G
A
D
S
T
Site 76
T690
S
L
D
G
A
D
S
T
G
V
V
A
K
L
S
Site 77
S697
T
G
V
V
A
K
L
S
P
A
N
Q
R
K
C
Site 78
T725
R
P
L
H
Q
L
A
T
D
S
T
F
S
L
D
Site 79
S727
L
H
Q
L
A
T
D
S
T
F
S
L
D
Q
P
Site 80
T728
H
Q
L
A
T
D
S
T
F
S
L
D
Q
P
G
Site 81
S730
L
A
T
D
S
T
F
S
L
D
Q
P
G
G
T
Site 82
T737
S
L
D
Q
P
G
G
T
L
D
L
T
L
I
R
Site 83
S752
A
R
L
Q
E
K
L
S
P
P
Y
S
S
P
Q
Site 84
Y755
Q
E
K
L
S
P
P
Y
S
S
P
Q
E
F
A
Site 85
S756
E
K
L
S
P
P
Y
S
S
P
Q
E
F
A
Q
Site 86
S757
K
L
S
P
P
Y
S
S
P
Q
E
F
A
Q
D
Site 87
S784
E
D
K
A
D
V
Q
S
I
I
G
L
Q
R
F
Site 88
S816
L
V
E
P
P
P
M
S
L
P
G
A
G
L
S
Site 89
S823
S
L
P
G
A
G
L
S
S
Q
E
L
S
G
G
Site 90
S824
L
P
G
A
G
L
S
S
Q
E
L
S
G
G
P
Site 91
S828
G
L
S
S
Q
E
L
S
G
G
P
G
D
G
P
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation