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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
DLK
Full Name:
Mitogen-activated protein kinase kinase kinase 12
Alias:
Dual leucine zipper bearing kinase; Dual leucine zipper kinase DLK; EC 2.7.11.25; Kinase DLK; Leucine-zipper protein kinase; M3K12; MAP3K12; MAPK-upstream kinase; MEKK12; MUK; ZPK; ZPKP1
Type:
EC 2.7.11.25; Protein kinase, Ser/Thr (non-receptor);
TKL
group; MLK family; LZK subfamily
Mass (Da):
93219
Number AA:
859
UniProt ID:
Q12852
International Prot ID:
IPI00292181
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0005829
GO:0019898
GO:0005624
Uniprot
OncoNet
Molecular Function:
GO:0005524
GO:0004709
GO:0000287
PhosphoSite+
KinaseNET
Biological Process:
GO:0007254
GO:0016572
GO:0018105
Phosida
TranscriptoNet
STRING
Kinexus Products
Mitogen-activated protein kinase kinase kinase 12 S269 phosphosite-specific antibody AB-PK590#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK590#Mitogen-activated protein kinase kinase kinase 12 (T266-G272, human) pS269 phosphopeptide - Powder PE-04ALS85#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04ALS85
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
T7
_
M
A
C
L
H
E
T
R
T
P
S
P
S
F
Site 2
T9
A
C
L
H
E
T
R
T
P
S
P
S
F
G
G
Site 3
S11
L
H
E
T
R
T
P
S
P
S
F
G
G
F
V
Site 4
S13
E
T
R
T
P
S
P
S
F
G
G
F
V
S
T
Site 5
S22
G
G
F
V
S
T
L
S
E
A
S
M
R
K
L
Site 6
S25
V
S
T
L
S
E
A
S
M
R
K
L
D
P
D
Site 7
T33
M
R
K
L
D
P
D
T
S
D
C
T
P
E
K
Site 8
S34
R
K
L
D
P
D
T
S
D
C
T
P
E
K
D
Site 9
T37
D
P
D
T
S
D
C
T
P
E
K
D
L
T
P
Site 10
T43
C
T
P
E
K
D
L
T
P
T
H
V
L
Q
L
Site 11
S64
G
P
G
G
A
A
G
S
P
E
S
R
A
S
R
Site 12
S67
G
A
A
G
S
P
E
S
R
A
S
R
V
R
A
Site 13
S70
G
S
P
E
S
R
A
S
R
V
R
A
D
E
V
Site 14
S83
E
V
R
L
Q
C
Q
S
G
S
G
F
L
E
G
Site 15
S85
R
L
Q
C
Q
S
G
S
G
F
L
E
G
L
F
Site 16
S107
T
M
I
G
K
A
Y
S
T
E
H
K
Q
Q
Q
Site 17
T160
K
V
R
D
L
K
E
T
D
I
K
H
L
R
K
Site 18
Y199
F
C
A
Q
G
Q
L
Y
E
V
L
R
A
G
R
Site 19
S239
I
I
H
R
D
L
K
S
P
N
M
L
I
T
Y
Site 20
T257
V
K
I
S
D
F
G
T
S
K
E
L
S
D
K
Site 21
S258
K
I
S
D
F
G
T
S
K
E
L
S
D
K
S
Site 22
S262
F
G
T
S
K
E
L
S
D
K
S
T
K
M
S
Site 23
S265
S
K
E
L
S
D
K
S
T
K
M
S
F
A
G
Site 24
T266
K
E
L
S
D
K
S
T
K
M
S
F
A
G
T
Site 25
S269
S
D
K
S
T
K
M
S
F
A
G
T
V
A
W
Mitogen-activated protein kinase kinase kinase 12 S269 phosphosite-specific antibody AB-PK590#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK590#Mitogen-activated protein kinase kinase kinase 12 (T266-G272, human) pS269 phosphopeptide - Powder PE-04ALS85#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04ALS85
Site 26
S288
V
I
R
N
E
P
V
S
E
K
V
D
I
W
S
Site 27
S316
P
Y
K
D
V
D
S
S
A
I
I
W
G
V
G
Site 28
S333
S
L
H
L
P
V
P
S
S
C
P
D
G
F
K
Site 29
S349
L
L
R
Q
C
W
N
S
K
P
R
N
R
P
S
Site 30
S356
S
K
P
R
N
R
P
S
F
R
Q
I
L
L
H
Site 31
T374
A
S
A
D
V
L
S
T
P
Q
E
T
Y
F
K
Site 32
Y379
L
S
T
P
Q
E
T
Y
F
K
S
Q
A
E
W
Site 33
S382
P
Q
E
T
Y
F
K
S
Q
A
E
W
R
E
E
Site 34
S399
L
H
F
E
K
I
K
S
E
G
T
C
L
H
R
Site 35
Y429
A
L
D
I
R
E
H
Y
E
R
K
L
E
R
A
Site 36
Y440
L
E
R
A
N
N
L
Y
M
E
L
N
A
L
M
Site 37
S477
G
L
L
K
P
H
P
S
R
G
L
L
H
G
N
Site 38
T485
R
G
L
L
H
G
N
T
M
E
K
L
I
K
K
Site 39
S500
R
N
V
P
Q
K
L
S
P
H
S
K
R
P
D
Site 40
S503
P
Q
K
L
S
P
H
S
K
R
P
D
I
L
K
Site 41
T511
K
R
P
D
I
L
K
T
E
S
L
L
P
K
L
Site 42
S513
P
D
I
L
K
T
E
S
L
L
P
K
L
D
A
Site 43
S536
G
C
P
K
G
P
P
S
P
G
R
S
R
R
G
Site 44
S540
G
P
P
S
P
G
R
S
R
R
G
K
T
R
H
Site 45
T545
G
R
S
R
R
G
K
T
R
H
R
K
A
S
A
Site 46
S551
K
T
R
H
R
K
A
S
A
K
G
S
C
G
D
Site 47
S555
R
K
A
S
A
K
G
S
C
G
D
L
P
G
L
Site 48
T564
G
D
L
P
G
L
R
T
A
V
P
P
H
E
P
Site 49
S576
H
E
P
G
G
P
G
S
P
G
G
L
G
G
G
Site 50
S585
G
G
L
G
G
G
P
S
A
W
E
A
C
P
P
Site 51
S607
D
L
L
L
R
K
M
S
S
S
S
P
D
L
L
Site 52
S608
L
L
L
R
K
M
S
S
S
S
P
D
L
L
S
Site 53
S609
L
L
R
K
M
S
S
S
S
P
D
L
L
S
A
Site 54
S610
L
R
K
M
S
S
S
S
P
D
L
L
S
A
A
Site 55
S615
S
S
S
P
D
L
L
S
A
A
L
G
S
R
G
Site 56
T626
G
S
R
G
R
G
A
T
G
G
A
G
D
P
G
Site 57
S634
G
G
A
G
D
P
G
S
P
P
P
A
R
G
D
Site 58
T642
P
P
P
A
R
G
D
T
P
P
S
E
G
S
A
Site 59
S645
A
R
G
D
T
P
P
S
E
G
S
A
P
G
S
Site 60
S648
D
T
P
P
S
E
G
S
A
P
G
S
T
S
P
Site 61
S652
S
E
G
S
A
P
G
S
T
S
P
D
S
P
G
Site 62
T653
E
G
S
A
P
G
S
T
S
P
D
S
P
G
G
Site 63
S654
G
S
A
P
G
S
T
S
P
D
S
P
G
G
A
Site 64
S657
P
G
S
T
S
P
D
S
P
G
G
A
K
G
E
Site 65
T680
E
G
V
G
L
L
G
T
G
R
E
G
T
S
G
Site 66
T685
L
G
T
G
R
E
G
T
S
G
R
G
G
S
R
Site 67
S686
G
T
G
R
E
G
T
S
G
R
G
G
S
R
A
Site 68
S691
G
T
S
G
R
G
G
S
R
A
G
S
Q
H
L
Site 69
S695
R
G
G
S
R
A
G
S
Q
H
L
T
P
A
A
Site 70
T699
R
A
G
S
Q
H
L
T
P
A
A
L
L
Y
R
Site 71
S712
Y
R
A
A
V
T
R
S
Q
K
R
G
I
S
S
Site 72
S718
R
S
Q
K
R
G
I
S
S
E
E
E
E
G
E
Site 73
S719
S
Q
K
R
G
I
S
S
E
E
E
E
G
E
V
Site 74
S728
E
E
E
G
E
V
D
S
E
V
E
L
T
S
S
Site 75
T733
V
D
S
E
V
E
L
T
S
S
Q
R
W
P
Q
Site 76
S734
D
S
E
V
E
L
T
S
S
Q
R
W
P
Q
S
Site 77
S735
S
E
V
E
L
T
S
S
Q
R
W
P
Q
S
L
Site 78
S741
S
S
Q
R
W
P
Q
S
L
N
M
R
Q
S
L
Site 79
S747
Q
S
L
N
M
R
Q
S
L
S
T
F
S
S
E
Site 80
S749
L
N
M
R
Q
S
L
S
T
F
S
S
E
N
P
Site 81
T750
N
M
R
Q
S
L
S
T
F
S
S
E
N
P
S
Site 82
S753
Q
S
L
S
T
F
S
S
E
N
P
S
D
G
E
Site 83
S757
T
F
S
S
E
N
P
S
D
G
E
E
G
T
A
Site 84
S765
D
G
E
E
G
T
A
S
E
P
S
P
S
G
T
Site 85
S768
E
G
T
A
S
E
P
S
P
S
G
T
P
E
V
Site 86
S770
T
A
S
E
P
S
P
S
G
T
P
E
V
G
S
Site 87
T772
S
E
P
S
P
S
G
T
P
E
V
G
S
T
N
Site 88
S777
S
G
T
P
E
V
G
S
T
N
T
D
E
R
P
Site 89
T778
G
T
P
E
V
G
S
T
N
T
D
E
R
P
D
Site 90
S788
D
E
R
P
D
E
R
S
D
D
M
C
S
Q
G
Site 91
S793
E
R
S
D
D
M
C
S
Q
G
S
E
I
P
L
Site 92
S805
I
P
L
D
P
P
P
S
E
V
I
P
G
P
E
Site 93
S814
V
I
P
G
P
E
P
S
S
L
P
I
P
H
Q
Site 94
S815
I
P
G
P
E
P
S
S
L
P
I
P
H
Q
E
Site 95
S832
R
E
R
G
P
P
N
S
E
D
S
D
C
D
S
Site 96
S835
G
P
P
N
S
E
D
S
D
C
D
S
T
E
L
Site 97
S839
S
E
D
S
D
C
D
S
T
E
L
D
N
S
N
Site 98
T840
E
D
S
D
C
D
S
T
E
L
D
N
S
N
S
Site 99
S845
D
S
T
E
L
D
N
S
N
S
V
D
A
L
R
Site 100
S847
T
E
L
D
N
S
N
S
V
D
A
L
R
P
P
Site 101
S856
D
A
L
R
P
P
A
S
L
P
P
_
_
_
_
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation