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Updated November 2019
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Protein Info
Short Name:
MLK1
Full Name:
Mitogen-activated protein kinase kinase kinase 9
Alias:
EC 2.7.11.25; M3K9; MAPKKK9; MLK-1; PRKE1
Type:
Protein-serine kinase, TKL group, MLK family, MLK subfamily
Mass (Da):
121895
Number AA:
1104
UniProt ID:
P80192
International Prot ID:
IPI00008115
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
Uniprot
OncoNet
Molecular Function:
GO:0005524
GO:0004706
GO:0004708
PhosphoSite+
KinaseNET
Biological Process:
GO:0007257
GO:0046777
Phosida
TranscriptoNet
STRING
Kinexus Products
Mixed-lineage protein-serine kinase 1; Mitogen-activated protein kinase kinase kinase 9 pan-specific antibody AB-NK278-1#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-NK278-1#Mixed-lineage protein-serine kinase 1 / Mitogen-activated protein kinase kinase kinase 9 (A1086-S1104, human) peptide - Powder PE-01BDX99, PE-01BDY95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01BDX99, PE-01BDY95
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S83
G
D
V
V
E
V
L
S
K
D
S
Q
V
S
G
Site 2
S86
V
E
V
L
S
K
D
S
Q
V
S
G
D
E
G
Site 3
S108
Q
R
V
G
I
F
P
S
N
Y
V
T
P
R
S
Site 4
T112
I
F
P
S
N
Y
V
T
P
R
S
A
F
S
S
Site 5
S115
S
N
Y
V
T
P
R
S
A
F
S
S
R
C
Q
Site 6
S118
V
T
P
R
S
A
F
S
S
R
C
Q
P
G
G
Site 7
S119
T
P
R
S
A
F
S
S
R
C
Q
P
G
G
E
Site 8
S129
Q
P
G
G
E
D
P
S
C
Y
P
P
I
Q
L
Site 9
Y131
G
G
E
D
P
S
C
Y
P
P
I
Q
L
L
E
Site 10
S182
H
D
P
D
E
D
I
S
Q
T
I
E
N
V
R
Site 11
S233
G
P
L
N
R
V
L
S
G
K
R
I
P
P
D
Site 12
Y255
Q
I
A
R
G
M
N
Y
L
H
D
E
A
I
V
Site 13
S272
I
H
R
D
L
K
S
S
N
I
L
I
L
Q
K
Site 14
S286
K
V
E
N
G
D
L
S
N
K
I
L
K
I
T
Site 15
T304
L
A
R
E
W
H
R
T
T
K
M
S
A
A
G
Site 16
T305
A
R
E
W
H
R
T
T
K
M
S
A
A
G
T
Site 17
S308
W
H
R
T
T
K
M
S
A
A
G
T
Y
A
W
Site 18
T312
T
K
M
S
A
A
G
T
Y
A
W
M
A
P
E
Site 19
S324
A
P
E
V
I
R
A
S
M
F
S
K
G
S
D
Site 20
S327
V
I
R
A
S
M
F
S
K
G
S
D
V
W
S
Site 21
S330
A
S
M
F
S
K
G
S
D
V
W
S
Y
G
V
Site 22
S334
S
K
G
S
D
V
W
S
Y
G
V
L
L
W
E
Site 23
S395
P
D
P
H
S
R
P
S
F
T
N
I
L
D
Q
Site 24
T404
T
N
I
L
D
Q
L
T
T
I
E
E
S
G
F
Site 25
T452
R
T
W
E
E
E
L
T
R
A
A
L
Q
Q
K
Site 26
S508
R
K
G
K
F
R
K
S
R
L
K
L
K
D
G
Site 27
S519
L
K
D
G
N
R
I
S
L
P
S
D
F
Q
H
Site 28
S522
G
N
R
I
S
L
P
S
D
F
Q
H
K
F
T
Site 29
T529
S
D
F
Q
H
K
F
T
V
Q
A
S
P
T
M
Site 30
S533
H
K
F
T
V
Q
A
S
P
T
M
D
K
R
K
Site 31
T535
F
T
V
Q
A
S
P
T
M
D
K
R
K
S
L
Site 32
S541
P
T
M
D
K
R
K
S
L
I
N
S
R
S
S
Site 33
S545
K
R
K
S
L
I
N
S
R
S
S
P
P
A
S
Site 34
S547
K
S
L
I
N
S
R
S
S
P
P
A
S
P
T
Site 35
S548
S
L
I
N
S
R
S
S
P
P
A
S
P
T
I
Site 36
S552
S
R
S
S
P
P
A
S
P
T
I
I
P
R
L
Site 37
T554
S
S
P
P
A
S
P
T
I
I
P
R
L
R
A
Site 38
T565
R
L
R
A
I
Q
L
T
P
G
E
S
S
K
T
Site 39
T572
T
P
G
E
S
S
K
T
W
G
R
S
S
V
V
Site 40
S577
S
K
T
W
G
R
S
S
V
V
P
K
E
E
G
Site 41
T598
A
P
K
K
K
G
R
T
W
G
P
G
T
L
G
Site 42
T603
G
R
T
W
G
P
G
T
L
G
Q
K
E
L
A
Site 43
S611
L
G
Q
K
E
L
A
S
G
D
E
G
S
P
Q
Site 44
S616
L
A
S
G
D
E
G
S
P
Q
R
R
E
K
A
Site 45
S627
R
E
K
A
N
G
L
S
T
P
S
E
S
P
H
Site 46
T628
E
K
A
N
G
L
S
T
P
S
E
S
P
H
F
Site 47
S630
A
N
G
L
S
T
P
S
E
S
P
H
F
H
L
Site 48
S632
G
L
S
T
P
S
E
S
P
H
F
H
L
G
L
Site 49
S641
H
F
H
L
G
L
K
S
L
V
D
G
Y
K
Q
Site 50
Y646
L
K
S
L
V
D
G
Y
K
Q
W
S
S
S
A
Site 51
S651
D
G
Y
K
Q
W
S
S
S
A
P
N
L
V
K
Site 52
S652
G
Y
K
Q
W
S
S
S
A
P
N
L
V
K
G
Site 53
S662
N
L
V
K
G
P
R
S
S
P
A
L
P
G
F
Site 54
S663
L
V
K
G
P
R
S
S
P
A
L
P
G
F
T
Site 55
S671
P
A
L
P
G
F
T
S
L
M
E
M
E
D
E
Site 56
S680
M
E
M
E
D
E
D
S
E
G
P
G
S
G
E
Site 57
S685
E
D
S
E
G
P
G
S
G
E
S
R
L
Q
H
Site 58
S688
E
G
P
G
S
G
E
S
R
L
Q
H
S
P
S
Site 59
S693
G
E
S
R
L
Q
H
S
P
S
Q
S
Y
L
C
Site 60
S695
S
R
L
Q
H
S
P
S
Q
S
Y
L
C
I
P
Site 61
S697
L
Q
H
S
P
S
Q
S
Y
L
C
I
P
F
P
Site 62
S713
G
E
D
G
D
G
P
S
S
D
G
I
H
E
E
Site 63
S714
E
D
G
D
G
P
S
S
D
G
I
H
E
E
P
Site 64
T722
D
G
I
H
E
E
P
T
P
V
N
S
A
T
S
Site 65
S726
E
E
P
T
P
V
N
S
A
T
S
T
P
Q
L
Site 66
T728
P
T
P
V
N
S
A
T
S
T
P
Q
L
T
P
Site 67
T730
P
V
N
S
A
T
S
T
P
Q
L
T
P
T
N
Site 68
T734
A
T
S
T
P
Q
L
T
P
T
N
S
L
K
R
Site 69
T736
S
T
P
Q
L
T
P
T
N
S
L
K
R
G
G
Site 70
S738
P
Q
L
T
P
T
N
S
L
K
R
G
G
A
H
Site 71
S801
R
E
G
L
F
Q
R
S
S
R
P
R
R
S
T
Site 72
S802
E
G
L
F
Q
R
S
S
R
P
R
R
S
T
S
Site 73
S807
R
S
S
R
P
R
R
S
T
S
P
P
S
R
K
Site 74
T808
S
S
R
P
R
R
S
T
S
P
P
S
R
K
L
Site 75
S809
S
R
P
R
R
S
T
S
P
P
S
R
K
L
F
Site 76
S812
R
R
S
T
S
P
P
S
R
K
L
F
K
K
E
Site 77
S841
L
L
S
L
S
S
I
S
E
C
N
S
T
R
S
Site 78
S845
S
S
I
S
E
C
N
S
T
R
S
L
L
R
S
Site 79
T846
S
I
S
E
C
N
S
T
R
S
L
L
R
S
D
Site 80
S848
S
E
C
N
S
T
R
S
L
L
R
S
D
S
D
Site 81
S852
S
T
R
S
L
L
R
S
D
S
D
E
I
V
V
Site 82
S854
R
S
L
L
R
S
D
S
D
E
I
V
V
Y
E
Site 83
S873
P
V
E
A
P
P
L
S
P
C
T
H
N
P
L
Site 84
S895
F
K
R
D
P
N
Q
S
L
T
P
T
H
V
T
Site 85
T897
R
D
P
N
Q
S
L
T
P
T
H
V
T
L
T
Site 86
T899
P
N
Q
S
L
T
P
T
H
V
T
L
T
T
P
Site 87
T904
T
P
T
H
V
T
L
T
T
P
S
Q
P
S
S
Site 88
T905
P
T
H
V
T
L
T
T
P
S
Q
P
S
S
H
Site 89
S907
H
V
T
L
T
T
P
S
Q
P
S
S
H
R
R
Site 90
S910
L
T
T
P
S
Q
P
S
S
H
R
R
T
P
S
Site 91
S911
T
T
P
S
Q
P
S
S
H
R
R
T
P
S
D
Site 92
T915
Q
P
S
S
H
R
R
T
P
S
D
G
A
L
K
Site 93
S917
S
S
H
R
R
T
P
S
D
G
A
L
K
P
E
Site 94
T925
D
G
A
L
K
P
E
T
L
L
A
S
R
S
P
Site 95
S929
K
P
E
T
L
L
A
S
R
S
P
S
S
N
G
Site 96
S931
E
T
L
L
A
S
R
S
P
S
S
N
G
L
S
Site 97
S933
L
L
A
S
R
S
P
S
S
N
G
L
S
P
S
Site 98
S934
L
A
S
R
S
P
S
S
N
G
L
S
P
S
P
Site 99
S938
S
P
S
S
N
G
L
S
P
S
P
G
A
G
M
Site 100
S940
S
S
N
G
L
S
P
S
P
G
A
G
M
L
K
Site 101
T948
P
G
A
G
M
L
K
T
P
S
P
S
R
D
P
Site 102
S950
A
G
M
L
K
T
P
S
P
S
R
D
P
G
E
Site 103
S952
M
L
K
T
P
S
P
S
R
D
P
G
E
F
P
Site 104
T971
P
N
V
V
F
P
P
T
P
R
R
W
N
T
Q
Site 105
T977
P
T
P
R
R
W
N
T
Q
Q
D
S
T
L
E
Site 106
T988
S
T
L
E
R
P
K
T
L
E
F
L
P
R
P
Site 107
S998
F
L
P
R
P
R
P
S
A
N
R
Q
R
L
D
Site 108
S1011
L
D
P
W
W
F
V
S
P
S
H
A
R
S
T
Site 109
S1013
P
W
W
F
V
S
P
S
H
A
R
S
T
S
P
Site 110
S1017
V
S
P
S
H
A
R
S
T
S
P
A
N
S
S
Site 111
T1018
S
P
S
H
A
R
S
T
S
P
A
N
S
S
S
Site 112
S1019
P
S
H
A
R
S
T
S
P
A
N
S
S
S
T
Site 113
S1023
R
S
T
S
P
A
N
S
S
S
T
E
T
P
S
Site 114
S1024
S
T
S
P
A
N
S
S
S
T
E
T
P
S
N
Site 115
S1025
T
S
P
A
N
S
S
S
T
E
T
P
S
N
L
Site 116
T1026
S
P
A
N
S
S
S
T
E
T
P
S
N
L
D
Site 117
T1028
A
N
S
S
S
T
E
T
P
S
N
L
D
S
C
Site 118
S1030
S
S
S
T
E
T
P
S
N
L
D
S
C
F
A
Site 119
S1034
E
T
P
S
N
L
D
S
C
F
A
S
S
S
S
Site 120
S1038
N
L
D
S
C
F
A
S
S
S
S
T
V
E
E
Site 121
S1041
S
C
F
A
S
S
S
S
T
V
E
E
R
P
G
Site 122
T1042
C
F
A
S
S
S
S
T
V
E
E
R
P
G
L
Site 123
T1063
Q
A
G
P
L
P
P
T
E
R
T
L
L
D
L
Site 124
S1079
A
E
G
Q
S
Q
D
S
T
V
P
L
C
R
A
Site 125
T1080
E
G
Q
S
Q
D
S
T
V
P
L
C
R
A
E
Site 126
T1090
L
C
R
A
E
L
N
T
H
R
P
A
P
Y
E
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation