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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
ENO1
Full Name:
Alpha-enolase
Alias:
2-phospho-D-glycerate hydro-lyase; Alpha enolase; EC 4.2.1.11; ENO-1; ENO1L1; ENO1P; ENOA; Enolase; Enolase, alpha; NNE; Non-neural enolase; Phosphopyruvate hydratase
Type:
EC 4.2.1.11; Transcription, coactivator/corepressor; Lyase; Carbohydrate Metabolism - glycolysis and gluconeogenesis
Mass (Da):
47169
Number AA:
434
UniProt ID:
P06733
International Prot ID:
IPI00465248
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0031430
GO:0005634
GO:0000015
Uniprot
OncoNet
Molecular Function:
GO:0000287
GO:0004634
GO:0004252
PhosphoSite+
KinaseNET
Biological Process:
GO:0006096
GO:0030308
GO:0000122
Phosida
TranscriptoNet
STRING
Kinexus Products
Alpha-enolase Y44 phosphosite-specific antibody AB-PN511#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PN511#Alpha-enolase (T41-L47, human) pY44 phosphopeptide - Powder PE-04ACS99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04ACS99
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S14
H
A
R
E
I
F
D
S
R
G
N
P
T
V
E
Site 2
T19
F
D
S
R
G
N
P
T
V
E
V
D
L
F
T
Site 3
T26
T
V
E
V
D
L
F
T
S
K
G
L
F
R
A
Site 4
S27
V
E
V
D
L
F
T
S
K
G
L
F
R
A
A
Site 5
S37
L
F
R
A
A
V
P
S
G
A
S
T
G
I
Y
Site 6
S40
A
A
V
P
S
G
A
S
T
G
I
Y
E
A
L
Site 7
T41
A
V
P
S
G
A
S
T
G
I
Y
E
A
L
E
Site 8
Y44
S
G
A
S
T
G
I
Y
E
A
L
E
L
R
D
Alpha-enolase Y44 phosphosite-specific antibody AB-PN511#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PN511#Alpha-enolase (T41-L47, human) pY44 phosphopeptide - Powder PE-04ACS99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04ACS99
Site 9
T55
E
L
R
D
N
D
K
T
R
Y
M
G
K
G
V
Site 10
Y57
R
D
N
D
K
T
R
Y
M
G
K
G
V
S
K
Site 11
S63
R
Y
M
G
K
G
V
S
K
A
V
E
H
I
N
Site 12
S79
T
I
A
P
A
L
V
S
K
K
L
N
V
T
E
Site 13
T100
L
M
I
E
M
D
G
T
E
N
K
S
K
F
G
Site 14
S104
M
D
G
T
E
N
K
S
K
F
G
A
N
A
I
Site 15
Y131
V
E
K
G
V
P
L
Y
R
H
I
A
D
L
A
Site 16
Y189
M
R
I
G
A
E
V
Y
H
N
L
K
N
V
I
Site 17
Y200
K
N
V
I
K
E
K
Y
G
K
D
A
T
N
V
Site 18
T205
E
K
Y
G
K
D
A
T
N
V
G
D
E
G
G
Site 19
Y236
T
A
I
G
K
A
G
Y
T
D
K
V
V
I
G
Site 20
T237
A
I
G
K
A
G
Y
T
D
K
V
V
I
G
M
Site 21
S249
I
G
M
D
V
A
A
S
E
F
F
R
S
G
K
Site 22
S254
A
A
S
E
F
F
R
S
G
K
Y
D
L
D
F
Site 23
Y257
E
F
F
R
S
G
K
Y
D
L
D
F
K
S
P
Site 24
S263
K
Y
D
L
D
F
K
S
P
D
D
P
S
R
Y
Site 25
S268
F
K
S
P
D
D
P
S
R
Y
I
S
P
D
Q
Site 26
Y270
S
P
D
D
P
S
R
Y
I
S
P
D
Q
L
A
Site 27
S272
D
D
P
S
R
Y
I
S
P
D
Q
L
A
D
L
Site 28
Y280
P
D
Q
L
A
D
L
Y
K
S
F
I
K
D
Y
Site 29
S282
Q
L
A
D
L
Y
K
S
F
I
K
D
Y
P
V
Site 30
Y287
Y
K
S
F
I
K
D
Y
P
V
V
S
I
E
D
Site 31
S291
I
K
D
Y
P
V
V
S
I
E
D
P
F
D
Q
Site 32
S310
A
W
Q
K
F
T
A
S
A
G
I
Q
V
V
G
Site 33
T321
Q
V
V
G
D
D
L
T
V
T
N
P
K
R
I
Site 34
T323
V
G
D
D
L
T
V
T
N
P
K
R
I
A
K
Site 35
S336
A
K
A
V
N
E
K
S
C
N
C
L
L
L
K
Site 36
S349
L
K
V
N
Q
I
G
S
V
T
E
S
L
Q
A
Site 37
S370
N
G
W
G
V
M
V
S
H
R
S
G
E
T
E
Site 38
T379
R
S
G
E
T
E
D
T
F
I
A
D
L
V
V
Site 39
T390
D
L
V
V
G
L
C
T
G
Q
I
K
T
G
A
Site 40
T395
L
C
T
G
Q
I
K
T
G
A
P
C
R
S
E
Site 41
S401
K
T
G
A
P
C
R
S
E
R
L
A
K
Y
N
Site 42
Y407
R
S
E
R
L
A
K
Y
N
Q
L
L
R
I
E
Site 43
S419
R
I
E
E
E
L
G
S
K
A
K
F
A
G
R
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation