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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
ROCK2
Full Name:
Rho-associated protein kinase 2
Alias:
EC 2.7.11.1; KIAA0619; Kinase ROCK2; P164 ROCK-2; RhoA-binding serine/threonine kinase alpha; Rho-associated protein kinase 2; Rho-associated, coiled-coil containing protein kinase 2; ROK-alpha
Type:
Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1;
AGC
group; DMPK family; ROCK subfamily
Mass (Da):
160900
Number AA:
1388
UniProt ID:
O75116
International Prot ID:
IPI00307155
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0005829
GO:0019898
GO:0005886
Uniprot
OncoNet
Molecular Function:
GO:0005524
GO:0005515
GO:0004674
PhosphoSite+
KinaseNET
Biological Process:
GO:0000910
GO:0007242
GO:0006468
Phosida
TranscriptoNet
STRING
Kinexus Products
Rho-associated protein kinase 2 Y722 phosphosite-specific antibody AB-PK799#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK799#Rock2Subtide - ROCK2 protein kinase substrate peptide - Powder PE-01BJE70#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01BJE70#Rho-associated protein kinase 2 (N719-I725, human) pY722 phosphopeptide - Powder PE-04ALB99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04ALB99
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S2
_
_
_
_
_
_
M
S
R
P
P
P
T
G
K
Site 2
T7
_
M
S
R
P
P
P
T
G
K
M
P
G
A
P
Site 3
T16
K
M
P
G
A
P
E
T
A
P
G
D
G
A
G
Site 4
S25
P
G
D
G
A
G
A
S
R
Q
R
K
L
E
A
Site 5
S39
A
L
I
R
D
P
R
S
P
I
N
V
E
S
L
Site 6
S45
R
S
P
I
N
V
E
S
L
L
D
G
L
N
S
Site 7
S114
Q
L
V
R
H
K
A
S
Q
K
V
Y
A
M
K
Site 8
Y118
H
K
A
S
Q
K
V
Y
A
M
K
L
L
S
K
Site 9
S134
E
M
I
K
R
S
D
S
A
F
F
W
E
E
R
Site 10
Y164
Y
A
F
Q
D
D
R
Y
L
Y
M
V
M
E
Y
Site 11
Y166
F
Q
D
D
R
Y
L
Y
M
V
M
E
Y
M
P
Site 12
S182
G
D
L
V
N
L
M
S
N
Y
D
V
P
E
K
Site 13
Y184
L
V
N
L
M
S
N
Y
D
V
P
E
K
W
A
Site 14
T235
L
K
L
A
D
F
G
T
C
M
K
M
D
E
T
Site 15
T249
T
G
M
V
H
C
D
T
A
V
G
T
P
D
Y
Site 16
T253
H
C
D
T
A
V
G
T
P
D
Y
I
S
P
E
Site 17
Y256
T
A
V
G
T
P
D
Y
I
S
P
E
V
L
K
Site 18
S258
V
G
T
P
D
Y
I
S
P
E
V
L
K
S
Q
Site 19
S264
I
S
P
E
V
L
K
S
Q
G
G
D
G
F
Y
Site 20
Y271
S
Q
G
G
D
G
F
Y
G
R
E
C
D
W
W
Site 21
S298
D
T
P
F
Y
A
D
S
L
V
G
T
Y
S
K
Site 22
T302
Y
A
D
S
L
V
G
T
Y
S
K
I
M
D
H
Site 23
S304
D
S
L
V
G
T
Y
S
K
I
M
D
H
K
N
Site 24
T367
H
W
D
N
I
R
E
T
A
A
P
V
V
P
E
Site 25
S376
A
P
V
V
P
E
L
S
S
D
I
D
S
S
N
Site 26
S377
P
V
V
P
E
L
S
S
D
I
D
S
S
N
F
Site 27
S381
E
L
S
S
D
I
D
S
S
N
F
D
D
I
E
Site 28
T396
D
D
K
G
D
V
E
T
F
P
I
P
K
A
F
Site 29
T414
Q
L
P
F
I
G
F
T
Y
Y
R
E
N
L
L
Site 30
S423
Y
R
E
N
L
L
L
S
D
S
P
S
C
R
E
Site 31
S425
E
N
L
L
L
S
D
S
P
S
C
R
E
N
D
Site 32
S427
L
L
L
S
D
S
P
S
C
R
E
N
D
S
I
Site 33
S433
P
S
C
R
E
N
D
S
I
Q
S
R
K
N
E
Site 34
S442
Q
S
R
K
N
E
E
S
Q
E
I
Q
K
K
L
Site 35
T451
E
I
Q
K
K
L
Y
T
L
E
E
H
L
S
N
Site 36
S457
Y
T
L
E
E
H
L
S
N
E
M
Q
A
K
E
Site 37
S472
E
L
E
Q
K
C
K
S
V
N
T
R
L
E
K
Site 38
T489
K
E
L
E
E
E
I
T
L
R
K
S
V
E
S
Site 39
S493
E
E
I
T
L
R
K
S
V
E
S
A
L
R
Q
Site 40
S496
T
L
R
K
S
V
E
S
A
L
R
Q
L
E
R
Site 41
Y515
L
Q
H
K
N
A
E
Y
Q
R
K
A
D
H
E
Site 42
S535
N
L
E
N
D
V
N
S
L
K
D
Q
L
E
D
Site 43
S550
L
K
K
R
N
Q
N
S
Q
I
S
T
E
K
V
Site 44
T554
N
Q
N
S
Q
I
S
T
E
K
V
N
Q
L
Q
Site 45
T567
L
Q
R
Q
L
D
E
T
N
A
L
L
R
T
E
Site 46
T573
E
T
N
A
L
L
R
T
E
S
D
T
A
A
R
Site 47
S575
N
A
L
L
R
T
E
S
D
T
A
A
R
L
R
Site 48
T577
L
L
R
T
E
S
D
T
A
A
R
L
R
K
T
Site 49
T584
T
A
A
R
L
R
K
T
Q
A
E
S
S
K
Q
Site 50
S625
K
E
F
I
N
L
Q
S
A
L
E
S
E
R
R
Site 51
S629
N
L
Q
S
A
L
E
S
E
R
R
D
R
T
H
Site 52
T635
E
S
E
R
R
D
R
T
H
G
S
E
I
I
N
Site 53
T677
R
Q
L
Q
E
R
F
T
D
L
E
K
E
K
S
Site 54
S684
T
D
L
E
K
E
K
S
N
M
E
I
D
M
T
Site 55
Y692
N
M
E
I
D
M
T
Y
Q
L
K
V
I
Q
Q
Site 56
S700
Q
L
K
V
I
Q
Q
S
L
E
Q
E
E
A
E
Site 57
T711
E
E
A
E
H
K
A
T
K
A
R
L
A
D
K
Site 58
Y722
L
A
D
K
N
K
I
Y
E
S
I
E
E
A
K
Rho-associated protein kinase 2 Y722 phosphosite-specific antibody AB-PK799#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK799#Rho-associated protein kinase 2 (N719-I725, human) pY722 phosphopeptide - Powder PE-04ALB99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04ALB99
Site 59
S724
D
K
N
K
I
Y
E
S
I
E
E
A
K
S
E
Site 60
S730
E
S
I
E
E
A
K
S
E
A
M
K
E
M
E
Site 61
S762
L
E
A
E
K
R
C
S
L
L
D
C
D
L
K
Site 62
S771
L
D
C
D
L
K
Q
S
Q
Q
K
I
N
E
L
Site 63
T793
N
E
D
V
R
N
L
T
L
K
I
E
Q
E
T
Site 64
T806
E
T
Q
K
R
C
L
T
Q
N
D
L
K
M
Q
Site 65
Y871
D
Q
L
E
A
E
Q
Y
F
S
T
L
Y
K
T
Site 66
S873
L
E
A
E
Q
Y
F
S
T
L
Y
K
T
Q
V
Site 67
Y876
E
Q
Y
F
S
T
L
Y
K
T
Q
V
R
E
L
Site 68
T878
Y
F
S
T
L
Y
K
T
Q
V
R
E
L
K
E
Site 69
S910
E
L
Q
D
E
R
D
S
L
A
A
Q
L
E
I
Site 70
T920
A
Q
L
E
I
T
L
T
K
A
D
S
E
Q
L
Site 71
S930
D
S
E
Q
L
A
R
S
I
A
E
E
Q
Y
S
Site 72
Y936
R
S
I
A
E
E
Q
Y
S
D
L
E
K
E
K
Site 73
S937
S
I
A
E
E
Q
Y
S
D
L
E
K
E
K
I
Site 74
T967
E
L
T
E
K
D
A
T
I
A
S
L
E
E
T
Site 75
S970
E
K
D
A
T
I
A
S
L
E
E
T
N
R
T
Site 76
T974
T
I
A
S
L
E
E
T
N
R
T
L
T
S
D
Site 77
T977
S
L
E
E
T
N
R
T
L
T
S
D
V
A
N
Site 78
T979
E
E
T
N
R
T
L
T
S
D
V
A
N
L
A
Site 79
S980
E
T
N
R
T
L
T
S
D
V
A
N
L
A
N
Site 80
T1026
Q
F
E
K
Q
L
L
T
E
R
T
L
K
T
Q
Site 81
T1054
P
V
K
R
G
N
D
T
D
V
R
R
K
E
K
Site 82
S1072
K
L
H
M
E
L
K
S
E
R
E
K
L
T
Q
Site 83
T1078
K
S
E
R
E
K
L
T
Q
Q
M
I
K
Y
Q
Site 84
Y1084
L
T
Q
Q
M
I
K
Y
Q
K
E
L
N
E
M
Site 85
S1099
Q
A
Q
I
A
E
E
S
Q
I
R
I
E
L
Q
Site 86
T1108
I
R
I
E
L
Q
M
T
L
D
S
K
D
S
D
Site 87
S1111
E
L
Q
M
T
L
D
S
K
D
S
D
I
E
Q
Site 88
S1114
M
T
L
D
S
K
D
S
D
I
E
Q
L
R
S
Site 89
S1121
S
D
I
E
Q
L
R
S
Q
L
Q
A
L
H
I
Site 90
S1132
A
L
H
I
G
L
D
S
S
S
I
G
S
G
P
Site 91
S1133
L
H
I
G
L
D
S
S
S
I
G
S
G
P
G
Site 92
S1134
H
I
G
L
D
S
S
S
I
G
S
G
P
G
D
Site 93
S1137
L
D
S
S
S
I
G
S
G
P
G
D
A
E
A
Site 94
S1151
A
D
D
G
F
P
E
S
R
L
E
G
W
L
S
Site 95
S1158
S
R
L
E
G
W
L
S
L
P
V
R
N
N
T
Site 96
T1165
S
L
P
V
R
N
N
T
K
K
F
G
W
V
K
Site 97
Y1174
K
F
G
W
V
K
K
Y
V
I
V
S
S
K
K
Site 98
Y1185
S
S
K
K
I
L
F
Y
D
S
E
Q
D
K
E
Site 99
S1187
K
K
I
L
F
Y
D
S
E
Q
D
K
E
Q
S
Site 100
T1212
L
F
H
V
R
P
V
T
Q
T
D
V
Y
R
A
Site 101
T1214
H
V
R
P
V
T
Q
T
D
V
Y
R
A
D
A
Site 102
Y1217
P
V
T
Q
T
D
V
Y
R
A
D
A
K
E
I
Site 103
Y1232
P
R
I
F
Q
I
L
Y
A
N
E
G
E
S
K
Site 104
Y1255
P
V
G
E
K
S
N
Y
I
C
H
K
G
H
E
Site 105
T1266
K
G
H
E
F
I
P
T
L
Y
H
F
P
T
N
Site 106
Y1268
H
E
F
I
P
T
L
Y
H
F
P
T
N
C
E
Site 107
Y1319
I
A
P
C
K
V
Y
Y
D
I
S
T
A
K
N
Site 108
S1333
N
L
L
L
L
A
N
S
T
E
E
Q
Q
K
W
Site 109
S1361
A
P
D
P
F
A
R
S
S
P
R
T
S
M
K
Site 110
S1362
P
D
P
F
A
R
S
S
P
R
T
S
M
K
I
Site 111
T1365
F
A
R
S
S
P
R
T
S
M
K
I
Q
Q
N
Site 112
S1366
A
R
S
S
P
R
T
S
M
K
I
Q
Q
N
Q
Site 113
S1374
M
K
I
Q
Q
N
Q
S
I
R
R
P
S
R
Q
Site 114
S1379
N
Q
S
I
R
R
P
S
R
Q
L
A
P
N
K
Site 115
S1388
Q
L
A
P
N
K
P
S
_
_
_
_
_
_
_
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation