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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
SPAG9
Full Name:
C-Jun-amino-terminal kinase-interacting protein 4
Alias:
HSS; JLP; JNK-associated leucine-zipper protein; JNK-interacting protein 4; MAPK8-interacting protein 4; PHET; PIG6; Sunday driver 1
Type:
Adapter/scaffold protein
Mass (Da):
146205
Number AA:
1321
UniProt ID:
O60271
International Prot ID:
IPI00744648
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0001669
GO:0016021
GO:0048471
Uniprot
OncoNet
Molecular Function:
GO:0005515
PhosphoSite+
KinaseNET
Biological Process:
GO:0030335
GO:0007283
Phosida
TranscriptoNet
STRING
Kinexus Products
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
Y9
E
L
E
D
G
V
V
Y
Q
E
E
P
G
G
S
Site 2
S16
Y
Q
E
E
P
G
G
S
G
A
V
M
S
E
R
Site 3
S30
R
V
S
G
L
A
G
S
I
Y
R
E
F
E
R
Site 4
Y32
S
G
L
A
G
S
I
Y
R
E
F
E
R
L
I
Site 5
Y42
F
E
R
L
I
G
R
Y
D
E
E
V
V
K
E
Site 6
S63
A
V
L
E
N
L
D
S
V
F
A
Q
D
Q
E
Site 7
T87
D
D
N
E
Q
L
I
T
Q
Y
E
R
E
K
A
Site 8
Y89
N
E
Q
L
I
T
Q
Y
E
R
E
K
A
L
R
Site 9
S109
K
F
I
E
F
E
D
S
Q
E
Q
E
K
K
D
Site 10
T119
Q
E
K
K
D
L
Q
T
R
V
E
S
L
E
S
Site 11
S123
D
L
Q
T
R
V
E
S
L
E
S
Q
T
R
Q
Site 12
S126
T
R
V
E
S
L
E
S
Q
T
R
Q
L
E
L
Site 13
Y138
L
E
L
K
A
K
N
Y
A
D
Q
I
S
R
L
Site 14
Y170
H
T
E
M
I
H
N
Y
M
E
H
L
E
R
T
Site 15
S183
R
T
K
L
H
Q
L
S
G
S
D
Q
L
E
S
Site 16
S185
K
L
H
Q
L
S
G
S
D
Q
L
E
S
T
A
Site 17
S190
S
G
S
D
Q
L
E
S
T
A
H
S
R
I
R
Site 18
S194
Q
L
E
S
T
A
H
S
R
I
R
K
E
R
P
Site 19
S203
I
R
K
E
R
P
I
S
L
G
I
F
P
L
P
Site 20
T217
P
A
G
D
G
L
L
T
P
D
A
Q
K
G
G
Site 21
T226
D
A
Q
K
G
G
E
T
P
G
S
E
Q
W
K
Site 22
S229
K
G
G
E
T
P
G
S
E
Q
W
K
F
Q
E
Site 23
S238
Q
W
K
F
Q
E
L
S
Q
P
R
S
H
T
S
Site 24
S242
Q
E
L
S
Q
P
R
S
H
T
S
L
K
V
S
Site 25
T244
L
S
Q
P
R
S
H
T
S
L
K
V
S
N
S
Site 26
S245
S
Q
P
R
S
H
T
S
L
K
V
S
N
S
P
Site 27
S249
S
H
T
S
L
K
V
S
N
S
P
E
P
Q
K
Site 28
S251
T
S
L
K
V
S
N
S
P
E
P
Q
K
A
V
Site 29
S265
V
E
Q
E
D
E
L
S
D
V
S
Q
G
G
S
Site 30
S268
E
D
E
L
S
D
V
S
Q
G
G
S
K
A
T
Site 31
S272
S
D
V
S
Q
G
G
S
K
A
T
T
P
A
S
Site 32
T275
S
Q
G
G
S
K
A
T
T
P
A
S
T
A
N
Site 33
T276
Q
G
G
S
K
A
T
T
P
A
S
T
A
N
S
Site 34
S279
S
K
A
T
T
P
A
S
T
A
N
S
D
V
A
Site 35
S283
T
P
A
S
T
A
N
S
D
V
A
T
I
P
T
Site 36
T287
T
A
N
S
D
V
A
T
I
P
T
D
T
P
L
Site 37
T290
S
D
V
A
T
I
P
T
D
T
P
L
K
E
E
Site 38
T292
V
A
T
I
P
T
D
T
P
L
K
E
E
N
E
Site 39
S311
V
T
D
A
P
N
K
S
E
I
S
K
H
I
E
Site 40
S329
A
Q
E
T
R
N
V
S
T
G
S
A
E
N
E
Site 41
T330
Q
E
T
R
N
V
S
T
G
S
A
E
N
E
E
Site 42
S332
T
R
N
V
S
T
G
S
A
E
N
E
E
K
S
Site 43
S339
S
A
E
N
E
E
K
S
E
V
Q
A
I
I
E
Site 44
S347
E
V
Q
A
I
I
E
S
T
P
E
L
D
M
D
Site 45
T348
V
Q
A
I
I
E
S
T
P
E
L
D
M
D
K
Site 46
S358
L
D
M
D
K
D
L
S
G
Y
K
G
S
S
T
Site 47
S363
D
L
S
G
Y
K
G
S
S
T
P
T
K
G
I
Site 48
S364
L
S
G
Y
K
G
S
S
T
P
T
K
G
I
E
Site 49
T365
S
G
Y
K
G
S
S
T
P
T
K
G
I
E
N
Site 50
T367
Y
K
G
S
S
T
P
T
K
G
I
E
N
K
A
Site 51
T379
N
K
A
F
D
R
N
T
E
S
L
F
E
E
L
Site 52
S381
A
F
D
R
N
T
E
S
L
F
E
E
L
S
S
Site 53
S387
E
S
L
F
E
E
L
S
S
A
G
S
G
L
I
Site 54
S388
S
L
F
E
E
L
S
S
A
G
S
G
L
I
G
Site 55
S391
E
E
L
S
S
A
G
S
G
L
I
G
D
V
D
Site 56
T418
E
N
L
I
L
E
N
T
Q
L
L
E
T
K
N
Site 57
T442
I
A
K
V
D
E
L
T
C
E
K
D
V
L
Q
Site 58
S493
K
A
K
D
D
D
D
S
D
I
P
T
A
Q
R
Site 59
T497
D
D
D
S
D
I
P
T
A
Q
R
K
R
F
T
Site 60
T504
T
A
Q
R
K
R
F
T
R
V
E
M
A
R
V
Site 61
Y518
V
L
M
E
R
N
Q
Y
K
E
R
L
M
E
L
Site 62
T532
L
Q
E
A
V
R
W
T
E
M
I
R
A
S
R
Site 63
S538
W
T
E
M
I
R
A
S
R
E
N
P
A
M
Q
Site 64
S551
M
Q
E
K
K
R
S
S
I
W
Q
F
F
S
R
Site 65
S557
S
S
I
W
Q
F
F
S
R
L
F
S
S
S
S
Site 66
S561
Q
F
F
S
R
L
F
S
S
S
S
N
T
T
K
Site 67
S562
F
F
S
R
L
F
S
S
S
S
N
T
T
K
K
Site 68
S563
F
S
R
L
F
S
S
S
S
N
T
T
K
K
P
Site 69
S564
S
R
L
F
S
S
S
S
N
T
T
K
K
P
E
Site 70
T566
L
F
S
S
S
S
N
T
T
K
K
P
E
P
P
Site 71
T567
F
S
S
S
S
N
T
T
K
K
P
E
P
P
V
Site 72
Y578
E
P
P
V
N
L
K
Y
N
A
P
T
S
H
V
Site 73
S583
L
K
Y
N
A
P
T
S
H
V
T
P
S
V
K
Site 74
T586
N
A
P
T
S
H
V
T
P
S
V
K
K
R
S
Site 75
S588
P
T
S
H
V
T
P
S
V
K
K
R
S
S
T
Site 76
S593
T
P
S
V
K
K
R
S
S
T
L
S
Q
L
P
Site 77
S594
P
S
V
K
K
R
S
S
T
L
S
Q
L
P
G
Site 78
T595
S
V
K
K
R
S
S
T
L
S
Q
L
P
G
D
Site 79
S597
K
K
R
S
S
T
L
S
Q
L
P
G
D
K
S
Site 80
S604
S
Q
L
P
G
D
K
S
K
A
F
D
F
L
S
Site 81
S611
S
K
A
F
D
F
L
S
E
E
T
E
A
S
L
Site 82
S617
L
S
E
E
T
E
A
S
L
A
S
R
R
E
Q
Site 83
S620
E
T
E
A
S
L
A
S
R
R
E
Q
K
R
E
Site 84
Y629
R
E
Q
K
R
E
Q
Y
R
Q
V
K
A
H
V
Site 85
Y654
G
W
S
L
P
Q
K
Y
K
Q
V
T
N
G
Q
Site 86
Y674
K
N
L
P
V
P
V
Y
L
R
P
L
D
E
K
Site 87
T683
R
P
L
D
E
K
D
T
S
M
K
L
W
C
A
Site 88
T700
V
N
L
S
G
G
K
T
R
D
G
G
S
V
V
Site 89
S705
G
K
T
R
D
G
G
S
V
V
G
A
S
V
F
Site 90
Y713
V
V
G
A
S
V
F
Y
K
D
V
A
G
L
D
Site 91
S724
A
G
L
D
T
E
G
S
K
Q
R
S
A
S
Q
Site 92
S728
T
E
G
S
K
Q
R
S
A
S
Q
S
S
L
D
Site 93
S730
G
S
K
Q
R
S
A
S
Q
S
S
L
D
K
L
Site 94
S732
K
Q
R
S
A
S
Q
S
S
L
D
K
L
D
Q
Site 95
S733
Q
R
S
A
S
Q
S
S
L
D
K
L
D
Q
E
Site 96
S756
K
N
Q
E
E
L
S
S
L
V
W
I
C
T
S
Site 97
T762
S
S
L
V
W
I
C
T
S
T
H
S
A
T
K
Site 98
S784
Q
P
G
N
I
L
D
S
F
T
V
C
N
S
H
Site 99
T804
S
V
P
G
A
R
E
T
D
Y
P
A
G
E
D
Site 100
Y806
P
G
A
R
E
T
D
Y
P
A
G
E
D
L
S
Site 101
S813
Y
P
A
G
E
D
L
S
E
S
G
Q
V
D
K
Site 102
S822
S
G
Q
V
D
K
A
S
L
C
G
S
M
T
S
Site 103
S826
D
K
A
S
L
C
G
S
M
T
S
N
S
S
A
Site 104
T828
A
S
L
C
G
S
M
T
S
N
S
S
A
E
T
Site 105
S829
S
L
C
G
S
M
T
S
N
S
S
A
E
T
D
Site 106
S831
C
G
S
M
T
S
N
S
S
A
E
T
D
S
L
Site 107
S832
G
S
M
T
S
N
S
S
A
E
T
D
S
L
L
Site 108
T835
T
S
N
S
S
A
E
T
D
S
L
L
G
G
I
Site 109
S858
G
V
T
G
A
A
T
S
P
S
T
N
G
A
S
Site 110
S860
T
G
A
A
T
S
P
S
T
N
G
A
S
P
V
Site 111
T861
G
A
A
T
S
P
S
T
N
G
A
S
P
V
M
Site 112
S865
S
P
S
T
N
G
A
S
P
V
M
D
K
P
P
Site 113
S879
P
E
M
E
A
E
N
S
E
V
D
E
N
V
P
Site 114
T887
E
V
D
E
N
V
P
T
A
E
E
A
T
E
A
Site 115
T905
N
A
G
S
A
E
D
T
V
D
I
S
Q
T
G
Site 116
S909
A
E
D
T
V
D
I
S
Q
T
G
V
Y
T
E
Site 117
Y914
D
I
S
Q
T
G
V
Y
T
E
H
V
F
T
D
Site 118
S932
V
Q
I
P
E
D
L
S
P
V
Y
Q
S
S
N
Site 119
Y935
P
E
D
L
S
P
V
Y
Q
S
S
N
D
S
D
Site 120
S937
D
L
S
P
V
Y
Q
S
S
N
D
S
D
A
Y
Site 121
S938
L
S
P
V
Y
Q
S
S
N
D
S
D
A
Y
K
Site 122
S941
V
Y
Q
S
S
N
D
S
D
A
Y
K
D
Q
I
Site 123
Y944
S
S
N
D
S
D
A
Y
K
D
Q
I
S
V
L
Site 124
S949
D
A
Y
K
D
Q
I
S
V
L
P
N
E
Q
D
Site 125
S967
E
E
A
Q
K
M
S
S
L
L
P
T
M
W
L
Site 126
T971
K
M
S
S
L
L
P
T
M
W
L
G
A
Q
N
Site 127
Y982
G
A
Q
N
G
C
L
Y
V
H
S
S
V
A
Q
Site 128
Y1038
G
Q
W
D
L
S
N
Y
H
L
L
D
L
G
R
Site 129
Y1069
C
G
Y
R
N
K
I
Y
V
V
Q
P
K
A
M
Site 130
S1081
K
A
M
K
I
E
K
S
F
D
A
H
P
R
K
Site 131
S1090
D
A
H
P
R
K
E
S
Q
V
R
Q
L
A
W
Site 132
S1110
W
V
S
I
R
L
D
S
T
L
R
L
Y
H
A
Site 133
T1111
V
S
I
R
L
D
S
T
L
R
L
Y
H
A
H
Site 134
Y1115
L
D
S
T
L
R
L
Y
H
A
H
T
Y
Q
H
Site 135
Y1131
Q
D
V
D
I
E
P
Y
V
S
K
M
L
G
T
Site 136
S1133
V
D
I
E
P
Y
V
S
K
M
L
G
T
G
K
Site 137
S1144
G
T
G
K
L
G
F
S
F
V
R
I
T
A
L
Site 138
T1175
I
S
I
P
L
T
E
T
N
K
T
S
G
V
P
Site 139
T1178
P
L
T
E
T
N
K
T
S
G
V
P
G
N
R
Site 140
S1188
V
P
G
N
R
P
G
S
V
I
R
V
Y
G
D
Site 141
Y1193
P
G
S
V
I
R
V
Y
G
D
E
N
S
D
K
Site 142
S1198
R
V
Y
G
D
E
N
S
D
K
V
T
P
G
T
Site 143
T1202
D
E
N
S
D
K
V
T
P
G
T
F
I
P
Y
Site 144
T1205
S
D
K
V
T
P
G
T
F
I
P
Y
C
S
M
Site 145
S1238
A
V
P
G
Q
V
I
S
P
Q
S
S
S
S
G
Site 146
S1241
G
Q
V
I
S
P
Q
S
S
S
S
G
T
D
L
Site 147
S1242
Q
V
I
S
P
Q
S
S
S
S
G
T
D
L
T
Site 148
S1244
I
S
P
Q
S
S
S
S
G
T
D
L
T
G
D
Site 149
T1246
P
Q
S
S
S
S
G
T
D
L
T
G
D
K
A
Site 150
T1249
S
S
S
G
T
D
L
T
G
D
K
A
G
P
S
Site 151
S1256
T
G
D
K
A
G
P
S
A
Q
E
P
G
S
Q
Site 152
S1262
P
S
A
Q
E
P
G
S
Q
T
P
L
K
S
M
Site 153
T1264
A
Q
E
P
G
S
Q
T
P
L
K
S
M
L
V
Site 154
Y1278
V
I
S
G
G
E
G
Y
I
D
F
R
M
G
D
Site 155
S1290
M
G
D
E
G
G
E
S
E
L
L
G
E
D
L
Site 156
S1302
E
D
L
P
L
E
P
S
V
T
K
A
E
R
S
Site 157
T1304
L
P
L
E
P
S
V
T
K
A
E
R
S
H
L
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation