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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
PLK4
Full Name:
Serine/threonine-protein kinase PLK4
Alias:
EC 2.7.11.21; PLK-4; Polo-like kinase 4; Sak; Serine,threonine-protein kinase 18; Serine/threonine protein kinase Sak; Serine/threonine-protein kinase 18; STK18
Type:
EC 2.7.11.21; Protein kinase, Ser/Thr (non-receptor); Other group; PLK family
Mass (Da):
108972
Number AA:
970
UniProt ID:
O00444
International Prot ID:
IPI00410344
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0005814
GO:0005829
GO:0005730
Uniprot
OncoNet
Molecular Function:
GO:0005524
GO:0005515
GO:0004674
PhosphoSite+
KinaseNET
Biological Process:
GO:0046601
GO:0006468
GO:0060707
Phosida
TranscriptoNet
STRING
Kinexus Products
Polo-like kinase-serine 4 (SAK) pan-specific antibody AB-NK291-1#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-NK291-1#Polo-like kinase-serine 4 (SAK) T170 phosphosite-specific antibody AB-PK780#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK780#Polo-like kinase-serine 4 (SAK) (E680-S698, human) peptide - Powder PE-01BFB90T#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01BFB90T#Polo-like kinase-serine 4 (SAK) (K167-G173, human) pT170 phosphopeptide - Powder PE-04AKZ99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AKZ99
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S22
G
N
L
L
G
K
G
S
F
A
G
V
Y
R
A
Site 2
Y27
K
G
S
F
A
G
V
Y
R
A
E
S
I
H
T
Site 3
Y76
H
P
S
I
L
E
L
Y
N
Y
F
E
D
S
N
Site 4
Y78
S
I
L
E
L
Y
N
Y
F
E
D
S
N
Y
V
Site 5
Y84
N
Y
F
E
D
S
N
Y
V
Y
L
V
L
E
M
Site 6
Y100
H
N
G
E
M
N
R
Y
L
K
N
R
V
K
P
Site 7
S109
K
N
R
V
K
P
F
S
E
N
E
A
R
H
F
Site 8
S140
L
H
R
D
L
T
L
S
N
L
L
L
T
R
N
Site 9
T159
I
A
D
F
G
L
A
T
Q
L
K
M
P
H
E
Site 10
Y169
K
M
P
H
E
K
H
Y
T
L
C
G
T
P
N
Site 11
T170
M
P
H
E
K
H
Y
T
L
C
G
T
P
N
Y
Polo-like kinase-serine 4 (SAK) T170 phosphosite-specific antibody AB-PK780#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK780#Polo-like kinase-serine 4 (SAK) (K167-G173, human) pT170 phosphopeptide - Powder PE-04AKZ99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AKZ99
Site 12
T174
K
H
Y
T
L
C
G
T
P
N
Y
I
S
P
E
Site 13
Y177
T
L
C
G
T
P
N
Y
I
S
P
E
I
A
T
Site 14
S179
C
G
T
P
N
Y
I
S
P
E
I
A
T
R
S
Site 15
S186
S
P
E
I
A
T
R
S
A
H
G
L
E
S
D
Site 16
S192
R
S
A
H
G
L
E
S
D
V
W
S
L
G
C
Site 17
T213
I
G
R
P
P
F
D
T
D
T
V
K
N
T
L
Site 18
T215
R
P
P
F
D
T
D
T
V
K
N
T
L
N
K
Site 19
S232
L
A
D
Y
E
M
P
S
F
L
S
I
E
A
K
Site 20
S255
R
N
P
A
D
R
L
S
L
S
S
V
L
D
H
Site 21
S257
P
A
D
R
L
S
L
S
S
V
L
D
H
P
F
Site 22
S258
A
D
R
L
S
L
S
S
V
L
D
H
P
F
M
Site 23
S266
V
L
D
H
P
F
M
S
R
N
S
S
T
K
S
Site 24
S269
H
P
F
M
S
R
N
S
S
T
K
S
K
D
L
Site 25
S270
P
F
M
S
R
N
S
S
T
K
S
K
D
L
G
Site 26
S273
S
R
N
S
S
T
K
S
K
D
L
G
T
V
E
Site 27
T278
T
K
S
K
D
L
G
T
V
E
D
S
I
D
S
Site 28
S282
D
L
G
T
V
E
D
S
I
D
S
G
H
A
T
Site 29
S285
T
V
E
D
S
I
D
S
G
H
A
T
I
S
T
Site 30
T289
S
I
D
S
G
H
A
T
I
S
T
A
I
T
A
Site 31
S291
D
S
G
H
A
T
I
S
T
A
I
T
A
S
S
Site 32
T292
S
G
H
A
T
I
S
T
A
I
T
A
S
S
S
Site 33
T295
A
T
I
S
T
A
I
T
A
S
S
S
T
S
I
Site 34
S297
I
S
T
A
I
T
A
S
S
S
T
S
I
S
G
Site 35
S298
S
T
A
I
T
A
S
S
S
T
S
I
S
G
S
Site 36
T300
A
I
T
A
S
S
S
T
S
I
S
G
S
L
F
Site 37
S301
I
T
A
S
S
S
T
S
I
S
G
S
L
F
D
Site 38
S303
A
S
S
S
T
S
I
S
G
S
L
F
D
K
R
Site 39
S305
S
S
T
S
I
S
G
S
L
F
D
K
R
R
L
Site 40
T323
Q
P
L
P
N
K
M
T
V
F
P
K
N
K
S
Site 41
S330
T
V
F
P
K
N
K
S
S
T
D
F
S
S
S
Site 42
S331
V
F
P
K
N
K
S
S
T
D
F
S
S
S
G
Site 43
T332
F
P
K
N
K
S
S
T
D
F
S
S
S
G
D
Site 44
S335
N
K
S
S
T
D
F
S
S
S
G
D
G
N
S
Site 45
S336
K
S
S
T
D
F
S
S
S
G
D
G
N
S
F
Site 46
S337
S
S
T
D
F
S
S
S
G
D
G
N
S
F
Y
Site 47
S342
S
S
S
G
D
G
N
S
F
Y
T
Q
W
G
N
Site 48
Y344
S
G
D
G
N
S
F
Y
T
Q
W
G
N
Q
E
Site 49
T345
G
D
G
N
S
F
Y
T
Q
W
G
N
Q
E
T
Site 50
S353
Q
W
G
N
Q
E
T
S
N
S
G
R
G
R
V
Site 51
S355
G
N
Q
E
T
S
N
S
G
R
G
R
V
I
Q
Site 52
S370
D
A
E
E
R
P
H
S
R
Y
L
R
R
A
Y
Site 53
Y372
E
E
R
P
H
S
R
Y
L
R
R
A
Y
S
S
Site 54
Y377
S
R
Y
L
R
R
A
Y
S
S
D
R
S
G
T
Site 55
S378
R
Y
L
R
R
A
Y
S
S
D
R
S
G
T
S
Site 56
S379
Y
L
R
R
A
Y
S
S
D
R
S
G
T
S
N
Site 57
S382
R
A
Y
S
S
D
R
S
G
T
S
N
S
Q
S
Site 58
T384
Y
S
S
D
R
S
G
T
S
N
S
Q
S
Q
A
Site 59
S385
S
S
D
R
S
G
T
S
N
S
Q
S
Q
A
K
Site 60
S387
D
R
S
G
T
S
N
S
Q
S
Q
A
K
T
Y
Site 61
S389
S
G
T
S
N
S
Q
S
Q
A
K
T
Y
T
M
Site 62
T393
N
S
Q
S
Q
A
K
T
Y
T
M
E
R
C
H
Site 63
Y394
S
Q
S
Q
A
K
T
Y
T
M
E
R
C
H
S
Site 64
S401
Y
T
M
E
R
C
H
S
A
E
M
L
S
V
S
Site 65
S406
C
H
S
A
E
M
L
S
V
S
K
R
S
G
G
Site 66
S408
S
A
E
M
L
S
V
S
K
R
S
G
G
G
E
Site 67
Y420
G
G
E
N
E
E
R
Y
S
P
T
D
N
N
A
Site 68
S421
G
E
N
E
E
R
Y
S
P
T
D
N
N
A
N
Site 69
T437
F
N
F
F
K
E
K
T
S
S
S
S
G
S
F
Site 70
S438
N
F
F
K
E
K
T
S
S
S
S
G
S
F
E
Site 71
S439
F
F
K
E
K
T
S
S
S
S
G
S
F
E
R
Site 72
S440
F
K
E
K
T
S
S
S
S
G
S
F
E
R
P
Site 73
S443
K
T
S
S
S
S
G
S
F
E
R
P
D
N
N
Site 74
S454
P
D
N
N
Q
A
L
S
N
H
L
C
P
G
K
Site 75
T462
N
H
L
C
P
G
K
T
P
F
P
F
A
D
P
Site 76
T470
P
F
P
F
A
D
P
T
P
Q
T
E
T
V
Q
Site 77
T473
F
A
D
P
T
P
Q
T
E
T
V
Q
Q
W
F
Site 78
T492
I
N
A
H
L
R
K
T
T
E
Y
D
S
I
S
Site 79
T493
N
A
H
L
R
K
T
T
E
Y
D
S
I
S
P
Site 80
Y495
H
L
R
K
T
T
E
Y
D
S
I
S
P
N
R
Site 81
S497
R
K
T
T
E
Y
D
S
I
S
P
N
R
D
F
Site 82
S499
T
T
E
Y
D
S
I
S
P
N
R
D
F
Q
G
Site 83
S515
P
D
L
Q
K
D
T
S
K
N
A
W
T
D
T
Site 84
T522
S
K
N
A
W
T
D
T
K
V
K
K
N
S
D
Site 85
S531
V
K
K
N
S
D
A
S
D
N
A
H
S
V
K
Site 86
Y545
K
Q
Q
N
T
M
K
Y
M
T
A
L
H
S
K
Site 87
T547
Q
N
T
M
K
Y
M
T
A
L
H
S
K
P
E
Site 88
S551
K
Y
M
T
A
L
H
S
K
P
E
I
I
Q
Q
Site 89
S564
Q
Q
E
C
V
F
G
S
D
P
L
S
E
Q
S
Site 90
S568
V
F
G
S
D
P
L
S
E
Q
S
K
T
R
G
Site 91
S571
S
D
P
L
S
E
Q
S
K
T
R
G
M
E
P
Site 92
T573
P
L
S
E
Q
S
K
T
R
G
M
E
P
P
W
Site 93
Y582
G
M
E
P
P
W
G
Y
Q
N
R
T
L
R
S
Site 94
T586
P
W
G
Y
Q
N
R
T
L
R
S
I
T
S
P
Site 95
S589
Y
Q
N
R
T
L
R
S
I
T
S
P
L
V
A
Site 96
T591
N
R
T
L
R
S
I
T
S
P
L
V
A
H
R
Site 97
S592
R
T
L
R
S
I
T
S
P
L
V
A
H
R
L
Site 98
T606
L
K
P
I
R
Q
K
T
K
K
A
V
V
S
I
Site 99
S612
K
T
K
K
A
V
V
S
I
L
D
S
E
E
V
Site 100
S629
E
L
V
K
E
Y
A
S
Q
E
Y
V
K
E
V
Site 101
Y632
K
E
Y
A
S
Q
E
Y
V
K
E
V
L
Q
I
Site 102
T645
Q
I
S
S
D
G
N
T
I
T
I
Y
Y
P
N
Site 103
T647
S
S
D
G
N
T
I
T
I
Y
Y
P
N
G
G
Site 104
Y649
D
G
N
T
I
T
I
Y
Y
P
N
G
G
R
G
Site 105
Y650
G
N
T
I
T
I
Y
Y
P
N
G
G
R
G
F
Site 106
S665
P
L
A
D
R
P
P
S
P
T
D
N
I
S
R
Site 107
T667
A
D
R
P
P
S
P
T
D
N
I
S
R
Y
S
Site 108
S671
P
S
P
T
D
N
I
S
R
Y
S
F
D
N
L
Site 109
Y673
P
T
D
N
I
S
R
Y
S
F
D
N
L
P
E
Site 110
S674
T
D
N
I
S
R
Y
S
F
D
N
L
P
E
K
Site 111
Y682
F
D
N
L
P
E
K
Y
W
R
K
Y
Q
Y
A
Site 112
Y686
P
E
K
Y
W
R
K
Y
Q
Y
A
S
R
F
V
Site 113
Y688
K
Y
W
R
K
Y
Q
Y
A
S
R
F
V
Q
L
Site 114
S698
R
F
V
Q
L
V
R
S
K
S
P
K
I
T
Y
Site 115
S700
V
Q
L
V
R
S
K
S
P
K
I
T
Y
F
T
Site 116
T704
R
S
K
S
P
K
I
T
Y
F
T
R
Y
A
K
Site 117
Y705
S
K
S
P
K
I
T
Y
F
T
R
Y
A
K
C
Site 118
S718
K
C
I
L
M
E
N
S
P
G
A
D
F
E
V
Site 119
Y728
A
D
F
E
V
W
F
Y
D
G
V
K
I
H
K
Site 120
T736
D
G
V
K
I
H
K
T
E
D
F
I
Q
V
I
Site 121
S749
V
I
E
K
T
G
K
S
Y
T
L
K
S
E
S
Site 122
T751
E
K
T
G
K
S
Y
T
L
K
S
E
S
E
V
Site 123
S754
G
K
S
Y
T
L
K
S
E
S
E
V
N
S
L
Site 124
S756
S
Y
T
L
K
S
E
S
E
V
N
S
L
K
E
Site 125
S760
K
S
E
S
E
V
N
S
L
K
E
E
I
K
M
Site 126
Y768
L
K
E
E
I
K
M
Y
M
D
H
A
N
E
G
Site 127
S784
R
I
C
L
A
L
E
S
I
I
S
E
E
E
R
Site 128
S787
L
A
L
E
S
I
I
S
E
E
E
R
K
T
R
Site 129
T793
I
S
E
E
E
R
K
T
R
S
A
P
F
F
P
Site 130
S795
E
E
E
R
K
T
R
S
A
P
F
F
P
I
I
Site 131
S809
I
I
G
R
K
P
G
S
T
S
S
P
K
A
L
Site 132
T810
I
G
R
K
P
G
S
T
S
S
P
K
A
L
S
Site 133
S811
G
R
K
P
G
S
T
S
S
P
K
A
L
S
P
Site 134
S812
R
K
P
G
S
T
S
S
P
K
A
L
S
P
P
Site 135
S817
T
S
S
P
K
A
L
S
P
P
P
S
V
D
S
Site 136
S821
K
A
L
S
P
P
P
S
V
D
S
N
Y
P
T
Site 137
S824
S
P
P
P
S
V
D
S
N
Y
P
T
R
E
R
Site 138
Y826
P
P
S
V
D
S
N
Y
P
T
R
E
R
A
S
Site 139
T828
S
V
D
S
N
Y
P
T
R
E
R
A
S
F
N
Site 140
S841
F
N
R
M
V
M
H
S
A
A
S
P
T
Q
A
Site 141
T846
M
H
S
A
A
S
P
T
Q
A
P
I
L
N
P
Site 142
T864
T
N
E
G
L
G
L
T
T
T
A
S
G
T
D
Site 143
T866
E
G
L
G
L
T
T
T
A
S
G
T
D
I
S
Site 144
S868
L
G
L
T
T
T
A
S
G
T
D
I
S
S
N
Site 145
T870
L
T
T
T
A
S
G
T
D
I
S
S
N
S
L
Site 146
S874
A
S
G
T
D
I
S
S
N
S
L
K
D
C
L
Site 147
S876
G
T
D
I
S
S
N
S
L
K
D
C
L
P
K
Site 148
S884
L
K
D
C
L
P
K
S
A
Q
L
L
K
S
V
Site 149
S915
W
V
Q
F
N
D
G
S
Q
L
V
V
Q
A
G
Site 150
S927
Q
A
G
V
S
S
I
S
Y
T
S
P
N
G
Q
Site 151
Y928
A
G
V
S
S
I
S
Y
T
S
P
N
G
Q
T
Site 152
T929
G
V
S
S
I
S
Y
T
S
P
N
G
Q
T
T
Site 153
S930
V
S
S
I
S
Y
T
S
P
N
G
Q
T
T
R
Site 154
Y938
P
N
G
Q
T
T
R
Y
G
E
N
E
K
L
P
Site 155
Y947
E
N
E
K
L
P
D
Y
I
K
Q
K
L
Q
C
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation