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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: SPAST All Species: 20.3
Human Site: S276 Identified Species: 34.36
UniProt: Q9UBP0 Number Species: 13
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q9UBP0 NP_055761.2 616 67197 S276 Y S G L S M V S G V K Q G S G
Chimpanzee Pan troglodytes
Rhesus Macaque Macaca mulatta
Dog Lupus familis XP_850973 624 68082 S284 C S G L S M V S G V R Q G P G
Cat Felis silvestris
Mouse Mus musculus Q9QYY8 614 66438 S274 C S G L S M V S G A R P G P G
Rat Rattus norvegicus B2RYN7 581 63003 S241 C S G L S M V S G A R P G S G
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001509194 572 63669 S232 C S G L S M F S A G R Q G A G
Chicken Gallus gallus Q5ZK92 613 66247 S273 Y S G I S T A S V S R P A A N
Frog Xenopus laevis Q6AZT2 600 65835 T260 P N C T S V P T S A R Q A G A
Zebra Danio Brachydanio rerio Q6NW58 570 63067 A229 L N C T P S A A Q S S R T G P
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster Q8I0P1 758 82731 A417 R Q P A K T A A T P P A V R R
Honey Bee Apis mellifera XP_393080 682 76301 S342 V A P I K P S S T P P S V K R
Nematode Worm Caenorhab. elegans Q8MNV0 512 56883 Q206 K A S L P M H Q N P V N R A A
Sea Urchin Strong. purpuratus O61577 516 57575 A210 D E K K F D P A G Y D K D L V
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q9SEX2 523 57218 P217 K R G L Y E G P D E D L A A M
Baker's Yeast Sacchar. cerevisiae P39955 897 100313 S491 S S S K K T S S H P S R P V S
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 N.A. N.A. 94.7 N.A. 92.5 88.9 N.A. 81.1 83.9 71 62.8 N.A. 42.8 46.9 33.7 30.3
Protein Similarity: 100 N.A. N.A. 95.8 N.A. 93.9 90 N.A. 84.4 88.4 80.8 73.3 N.A. 55.2 62 49.3 46.7
P-Site Identity: 100 N.A. N.A. 80 N.A. 66.6 73.3 N.A. 60 33.3 13.3 0 N.A. 0 6.6 13.3 6.6
P-Site Similarity: 100 N.A. N.A. 86.6 N.A. 73.3 80 N.A. 73.3 53.3 40 20 N.A. 6.6 20 26.6 20
Percent
Protein Identity: N.A. N.A. N.A. 31 30.1 N.A.
Protein Similarity: N.A. N.A. N.A. 49.3 45.8 N.A.
P-Site Identity: N.A. N.A. N.A. 13.3 13.3 N.A.
P-Site Similarity: N.A. N.A. N.A. 20 20 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 15 0 8 0 0 22 22 8 22 0 8 22 29 15 % A
% Cys: 29 0 15 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 8 0 0 0 0 8 0 0 8 0 15 0 8 0 0 % D
% Glu: 0 8 0 0 0 8 0 0 0 8 0 0 0 0 0 % E
% Phe: 0 0 0 0 8 0 8 0 0 0 0 0 0 0 0 % F
% Gly: 0 0 50 0 0 0 8 0 36 8 0 0 36 15 36 % G
% His: 0 0 0 0 0 0 8 0 8 0 0 0 0 0 0 % H
% Ile: 0 0 0 15 0 0 0 0 0 0 0 0 0 0 0 % I
% Lys: 15 0 8 15 22 0 0 0 0 0 8 8 0 8 0 % K
% Leu: 8 0 0 50 0 0 0 0 0 0 0 8 0 8 0 % L
% Met: 0 0 0 0 0 43 0 0 0 0 0 0 0 0 8 % M
% Asn: 0 15 0 0 0 0 0 0 8 0 0 8 0 0 8 % N
% Pro: 8 0 15 0 15 8 15 8 0 29 15 22 8 15 8 % P
% Gln: 0 8 0 0 0 0 0 8 8 0 0 29 0 0 0 % Q
% Arg: 8 8 0 0 0 0 0 0 0 0 43 15 8 8 15 % R
% Ser: 8 50 15 0 50 8 15 58 8 15 15 8 0 15 8 % S
% Thr: 0 0 0 15 0 22 0 8 15 0 0 0 8 0 0 % T
% Val: 8 0 0 0 0 8 29 0 8 15 8 0 15 8 8 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 15 0 0 0 8 0 0 0 0 8 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _