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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated: 2017 Aug. 1
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Warning
– Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite
Conservation Score
Human Protein:
NGLY1
All Species:
30
Human Site:
T403
Identified Species:
44
UniProt:
Q96IV0
Number Species:
15
Phosphosite Substitution
Charge Score:
-0.13
Phosphosite
Sequences
Species
Species
Scientific Name
UniProt ID
NCBI Ref Seq ID
AA#
Mr(Da)
P-Site
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Human
Homo sapiens
Q96IV0
NP_001138765.1
654
74390
T403
E
E
V
I
A
R
R
T
K
V
K
E
A
L
L
Chimpanzee
Pan troglodytes
XP_516333
835
93255
T584
E
E
V
I
A
R
R
T
K
V
K
E
A
L
L
Rhesus Macaque
Macaca mulatta
XP_001093251
654
74564
T403
E
E
V
I
A
R
R
T
K
V
K
E
A
L
L
Dog
Lupus familis
XP_534241
2041
231068
I544
R
D
K
N
M
T
C
I
K
V
S
I
D
P
E
Cat
Felis silvestris
Mouse
Mus musculus
Q9JI78
651
74257
T400
D
E
V
M
S
R
R
T
K
V
K
E
E
L
L
Rat
Rattus norvegicus
Q5XI55
651
74658
T400
E
E
V
M
S
R
R
T
K
V
K
E
E
L
L
Wallaby
Macropus eugenll
Platypus
Ornith. anatinus
XP_001507250
647
73725
T396
K
D
V
I
S
R
R
T
Q
I
T
E
D
Y
L
Chicken
Gallus gallus
Q5ZJM3
651
73884
T399
Q
E
V
L
T
R
R
T
A
L
S
E
A
K
L
Frog
Xenopus laevis
NP_001116357
635
72527
K384
E
D
V
I
A
R
R
K
E
V
R
E
S
W
L
Zebra Danio
Brachydanio rerio
Q503I8
644
72470
T388
P
E
V
L
S
R
R
T
Q
V
Q
E
T
W
L
Tiger Blowfish
Takifugu rubipres
Fruit Fly
Dros. melanogaster
Q7KRR5
631
73298
L386
T
N
D
H
Q
K
I
L
H
L
R
K
L
C
G
Honey Bee
Apis mellifera
XP_392561
589
68873
F347
K
L
S
Y
I
I
A
F
S
K
D
E
V
Q
D
Nematode Worm
Caenorhab. elegans
Q9TW67
606
69129
A364
S
K
L
N
S
R
Q
A
E
G
Q
T
E
P
R
Sea Urchin
Strong. purpuratus
Poplar Tree
Populus trichocarpa
Maize
Zea mays
Rice
Oryza sativa
Thale Cress
Arabidopsis thaliana
Q9FGY9
721
82428
T452
D
S
F
L
P
I
L
T
Q
F
V
E
D
G
L
Baker's Yeast
Sacchar. cerevisiae
Q02890
363
42466
F121
R
Y
F
K
Q
D
F
F
K
W
C
N
K
P
D
Red Bread Mold
Neurospora crassa
Q7SI01
382
43903
R140
V
A
P
T
P
E
E
R
A
H
S
A
T
W
V
Conservation
Percent
Protein Identity:
100
72.8
96.9
22.5
N.A.
84.5
84.4
N.A.
70.6
68.8
64.2
57.1
N.A.
32.8
35.1
31.3
N.A.
Protein Similarity:
100
74.2
98.9
27.3
N.A.
91.4
90.3
N.A.
81
81.3
78.1
72.6
N.A.
53.2
53
49
N.A.
P-Site Identity:
100
100
100
13.3
N.A.
73.3
80
N.A.
46.6
53.3
60
53.3
N.A.
0
6.6
6.6
N.A.
P-Site Similarity:
100
100
100
20
N.A.
93.3
93.3
N.A.
80
73.3
86.6
80
N.A.
26.6
13.3
46.6
N.A.
Percent
Protein Identity:
N.A.
N.A.
N.A.
29.9
25.9
23.2
Protein Similarity:
N.A.
N.A.
N.A.
46.6
37.7
36.3
P-Site Identity:
N.A.
N.A.
N.A.
20
6.6
0
P-Site Similarity:
N.A.
N.A.
N.A.
40
6.6
6.6
Phosphosite
Consensus
Position
-7
-6
-5
-4
-3
-4
-5
0
+1
+2
+3
+4
+5
+6
+7
% Ala:
0
7
0
0
25
0
7
7
13
0
0
7
25
0
0
% A
% Cys:
0
0
0
0
0
0
7
0
0
0
7
0
0
7
0
% C
% Asp:
13
19
7
0
0
7
0
0
0
0
7
0
19
0
13
% D
% Glu:
32
44
0
0
0
7
7
0
13
0
0
69
19
0
7
% E
% Phe:
0
0
13
0
0
0
7
13
0
7
0
0
0
0
0
% F
% Gly:
0
0
0
0
0
0
0
0
0
7
0
0
0
7
7
% G
% His:
0
0
0
7
0
0
0
0
7
7
0
0
0
0
0
% H
% Ile:
0
0
0
32
7
13
7
7
0
7
0
7
0
0
0
% I
% Lys:
13
7
7
7
0
7
0
7
44
7
32
7
7
7
0
% K
% Leu:
0
7
7
19
0
0
7
7
0
13
0
0
7
32
63
% L
% Met:
0
0
0
13
7
0
0
0
0
0
0
0
0
0
0
% M
% Asn:
0
7
0
13
0
0
0
0
0
0
0
7
0
0
0
% N
% Pro:
7
0
7
0
13
0
0
0
0
0
0
0
0
19
0
% P
% Gln:
7
0
0
0
13
0
7
0
19
0
13
0
0
7
0
% Q
% Arg:
13
0
0
0
0
63
57
7
0
0
13
0
0
0
7
% R
% Ser:
7
7
7
0
32
0
0
0
7
0
19
0
7
0
0
% S
% Thr:
7
0
0
7
7
7
0
57
0
0
7
7
13
0
0
% T
% Val:
7
0
57
0
0
0
0
0
0
50
7
0
7
0
7
% V
% Trp:
0
0
0
0
0
0
0
0
0
7
0
0
0
19
0
% W
% Tyr:
0
7
0
7
0
0
0
0
0
0
0
0
0
7
0
% Y
% Spaces:
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
% _