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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ATG4C All Species: 17.58
Human Site: S166 Identified Species: 27.62
UniProt: Q96DT6 Number Species: 14
    Phosphosite Substitution
    Charge Score: -0.14
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q96DT6 NP_116241.2 458 52497 S166 A S F E A S L S G E R E F K T
Chimpanzee Pan troglodytes XP_001159697 366 41520 Y91 I S R I W L T Y R E E F P Q I
Rhesus Macaque Macaca mulatta XP_001086218 458 52551 S166 A S F E A S L S G E R E L K T
Dog Lupus familis XP_852273 458 52523 S166 A S F E A S L S G E S E L K T
Cat Felis silvestris
Mouse Mus musculus Q811C2 458 52068 S166 A S F E A S L S G D R E L R T
Rat Rattus norvegicus NP_001101418 458 52091 S166 A S F E A S L S G E R E L R T
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001514370 459 52127 V166 A S F E A S W V G E R D P R P
Chicken Gallus gallus Q6PZ02 393 44512 K118 L N A F I D K K D S Y Y S I H
Frog Xenopus laevis Q5XH30 450 51749 P167 P S L E K S S P E N E E Y V S
Zebra Danio Brachydanio rerio Q6DG88 394 44435 K119 L N A F I D K K D S Y Y S I H
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_650452 668 74736 P229 S T S T S L Y P A L N P Q Q I
Honey Bee Apis mellifera XP_393739 477 54008 L181 Q M M L A Q A L V C H F L G R
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_786847 723 81931 M421 C M L R S G Q M M L A H S L I
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q8S929 467 51510 I172 D V N W G C M I R S S Q M L F
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa Q7S3X7 506 55337 C226 S S D T G W G C M I R S G Q S
Conservation
Percent
Protein Identity: 100 79.9 98.2 94.5 N.A. 90.3 89.5 N.A. 83.2 28.1 68.1 26.8 N.A. 26.5 30.3 N.A. 28
Protein Similarity: 100 79.9 98.9 97.3 N.A. 95.6 95.4 N.A. 88 42.5 82.7 46 N.A. 42 46.3 N.A. 41.4
P-Site Identity: 100 13.3 93.3 86.6 N.A. 80 86.6 N.A. 60 0 26.6 0 N.A. 0 6.6 N.A. 0
P-Site Similarity: 100 20 93.3 86.6 N.A. 93.3 93.3 N.A. 73.3 6.6 40 6.6 N.A. 26.6 6.6 N.A. 6.6
Percent
Protein Identity: N.A. N.A. N.A. 22.7 N.A. 21.5
Protein Similarity: N.A. N.A. N.A. 41.1 N.A. 39.3
P-Site Identity: N.A. N.A. N.A. 0 N.A. 13.3
P-Site Similarity: N.A. N.A. N.A. 13.3 N.A. 33.3
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 40 0 14 0 47 0 7 0 7 0 7 0 0 0 0 % A
% Cys: 7 0 0 0 0 7 0 7 0 7 0 0 0 0 0 % C
% Asp: 7 0 7 0 0 14 0 0 14 7 0 7 0 0 0 % D
% Glu: 0 0 0 47 0 0 0 0 7 40 14 40 0 0 0 % E
% Phe: 0 0 40 14 0 0 0 0 0 0 0 14 7 0 7 % F
% Gly: 0 0 0 0 14 7 7 0 40 0 0 0 7 7 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 7 7 0 0 14 % H
% Ile: 7 0 0 7 14 0 0 7 0 7 0 0 0 14 20 % I
% Lys: 0 0 0 0 7 0 14 14 0 0 0 0 0 20 0 % K
% Leu: 14 0 14 7 0 14 34 7 0 14 0 0 34 14 0 % L
% Met: 0 14 7 0 0 0 7 7 14 0 0 0 7 0 0 % M
% Asn: 0 14 7 0 0 0 0 0 0 7 7 0 0 0 0 % N
% Pro: 7 0 0 0 0 0 0 14 0 0 0 7 14 0 7 % P
% Gln: 7 0 0 0 0 7 7 0 0 0 0 7 7 20 0 % Q
% Arg: 0 0 7 7 0 0 0 0 14 0 40 0 0 20 7 % R
% Ser: 14 60 7 0 14 47 7 34 0 20 14 7 20 0 14 % S
% Thr: 0 7 0 14 0 0 7 0 0 0 0 0 0 0 34 % T
% Val: 0 7 0 0 0 0 0 7 7 0 0 0 0 7 0 % V
% Trp: 0 0 0 7 7 7 7 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 7 7 0 0 14 14 7 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _