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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated: 2017 Aug. 1
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Warning
– Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite
Conservation Score
Human Protein:
NSUN4
All Species:
30.3
Human Site:
Y249
Identified Species:
51.28
UniProt:
Q96CB9
Number Species:
13
Phosphosite Substitution
Charge Score:
-0.23
Phosphosite
Sequences
Species
Species
Scientific Name
UniProt ID
NCBI Ref Seq ID
AA#
Mr(Da)
P-Site
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Human
Homo sapiens
Q96CB9
NP_950245.2
384
43089
Y249
G
E
L
E
G
D
T
Y
D
R
V
L
V
D
V
Chimpanzee
Pan troglodytes
XP_513142
833
91470
Y249
G
E
L
E
G
D
T
Y
D
R
V
L
V
D
V
Rhesus Macaque
Macaca mulatta
XP_001108301
384
43099
Y249
G
E
L
E
G
D
T
Y
D
R
V
L
V
D
V
Dog
Lupus familis
XP_853047
384
43059
Y249
G
E
L
E
G
D
T
Y
D
R
V
L
V
D
V
Cat
Felis silvestris
Mouse
Mus musculus
Q9CZ57
381
42835
Y246
G
E
L
E
G
D
T
Y
D
R
V
L
V
D
V
Rat
Rattus norvegicus
NP_001100148
381
42594
Y246
G
E
L
E
G
D
T
Y
D
K
V
L
V
D
V
Wallaby
Macropus eugenll
Platypus
Ornith. anatinus
XP_001518738
248
26969
M126
G
L
L
E
Y
Y
L
M
D
A
A
S
L
L
P
Chicken
Gallus gallus
XP_422449
404
44758
K272
L
Q
G
G
T
F
H
K
V
L
V
D
V
P
C
Frog
Xenopus laevis
Q5M7E3
406
46042
Y271
G
D
L
E
A
S
T
Y
D
R
V
L
V
D
V
Zebra Danio
Brachydanio rerio
Q4KMK0
367
41244
K238
M
Q
P
G
A
Y
D
K
V
L
V
D
A
P
C
Tiger Blowfish
Takifugu rubipres
Fruit Fly
Dros. melanogaster
NP_608573
503
57519
F366
N
L
D
Q
Y
E
Q
F
D
K
I
L
V
D
V
Honey Bee
Apis mellifera
XP_394395
493
56346
K359
N
E
N
G
R
Y
N
K
I
L
V
D
V
P
C
Nematode Worm
Caenorhab. elegans
NP_490958
465
51501
A328
K
T
W
D
E
F
E
A
Y
D
K
V
L
V
D
Sea Urchin
Strong. purpuratus
XP_792601
513
57508
Y378
G
Q
I
E
P
M
A
Y
D
K
V
L
V
D
V
Poplar Tree
Populus trichocarpa
Maize
Zea mays
Rice
Oryza sativa
Thale Cress
Arabidopsis thaliana
Baker's Yeast
Sacchar. cerevisiae
Red Bread Mold
Neurospora crassa
Conservation
Percent
Protein Identity:
100
44.7
96.6
84.9
N.A.
85.9
83.5
N.A.
38.5
61.1
59.3
35.1
N.A.
32.5
32.4
31.3
35.4
Protein Similarity:
100
45.9
98.4
91.4
N.A.
91.9
90.6
N.A.
47.9
74.5
72.6
54.1
N.A.
48.3
47.6
46.8
49.3
P-Site Identity:
100
100
100
100
N.A.
100
93.3
N.A.
26.6
13.3
80
6.6
N.A.
33.3
20
0
60
P-Site Similarity:
100
100
100
100
N.A.
100
100
N.A.
33.3
20
86.6
13.3
N.A.
66.6
20
20
80
Percent
Protein Identity:
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
Protein Similarity:
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
P-Site Identity:
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
P-Site Similarity:
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
Phosphosite
Consensus
Position
-7
-6
-5
-4
-3
-4
-5
0
+1
+2
+3
+4
+5
+6
+7
% Ala:
0
0
0
0
15
0
8
8
0
8
8
0
8
0
0
% A
% Cys:
0
0
0
0
0
0
0
0
0
0
0
0
0
0
22
% C
% Asp:
0
8
8
8
0
43
8
0
72
8
0
22
0
65
8
% D
% Glu:
0
50
0
65
8
8
8
0
0
0
0
0
0
0
0
% E
% Phe:
0
0
0
0
0
15
0
8
0
0
0
0
0
0
0
% F
% Gly:
65
0
8
22
43
0
0
0
0
0
0
0
0
0
0
% G
% His:
0
0
0
0
0
0
8
0
0
0
0
0
0
0
0
% H
% Ile:
0
0
8
0
0
0
0
0
8
0
8
0
0
0
0
% I
% Lys:
8
0
0
0
0
0
0
22
0
22
8
0
0
0
0
% K
% Leu:
8
15
58
0
0
0
8
0
0
22
0
65
15
8
0
% L
% Met:
8
0
0
0
0
8
0
8
0
0
0
0
0
0
0
% M
% Asn:
15
0
8
0
0
0
8
0
0
0
0
0
0
0
0
% N
% Pro:
0
0
8
0
8
0
0
0
0
0
0
0
0
22
8
% P
% Gln:
0
22
0
8
0
0
8
0
0
0
0
0
0
0
0
% Q
% Arg:
0
0
0
0
8
0
0
0
0
43
0
0
0
0
0
% R
% Ser:
0
0
0
0
0
8
0
0
0
0
0
8
0
0
0
% S
% Thr:
0
8
0
0
8
0
50
0
0
0
0
0
0
0
0
% T
% Val:
0
0
0
0
0
0
0
0
15
0
79
8
79
8
65
% V
% Trp:
0
0
8
0
0
0
0
0
0
0
0
0
0
0
0
% W
% Tyr:
0
0
0
0
15
22
0
58
8
0
0
0
0
0
0
% Y
% Spaces:
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
% _