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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated: 2017 Aug. 1
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Warning
– Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite
Conservation Score
Human Protein:
NSUN4
All Species:
24.85
Human Site:
T133
Identified Species:
42.05
UniProt:
Q96CB9
Number Species:
13
Phosphosite Substitution
Charge Score:
-0.08
Phosphosite
Sequences
Species
Species
Scientific Name
UniProt ID
NCBI Ref Seq ID
AA#
Mr(Da)
P-Site
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Human
Homo sapiens
Q96CB9
NP_950245.2
384
43089
T133
S
P
N
L
R
C
F
T
F
D
R
G
D
I
S
Chimpanzee
Pan troglodytes
XP_513142
833
91470
T133
S
P
N
L
R
C
F
T
F
D
R
G
D
I
S
Rhesus Macaque
Macaca mulatta
XP_001108301
384
43099
T133
S
P
N
L
R
C
F
T
F
A
R
G
D
V
S
Dog
Lupus familis
XP_853047
384
43059
T133
S
P
N
L
R
C
F
T
F
A
R
G
D
V
S
Cat
Felis silvestris
Mouse
Mus musculus
Q9CZ57
381
42835
R133
L
R
C
F
T
F
S
R
G
D
V
S
R
F
P
Rat
Rattus norvegicus
NP_001100148
381
42594
K133
L
R
C
F
T
F
S
K
G
D
V
S
R
F
P
Wallaby
Macropus eugenll
Platypus
Ornith. anatinus
XP_001518738
248
26969
N20
A
L
Q
N
F
D
V
N
Y
S
V
Q
F
G
D
Chicken
Gallus gallus
XP_422449
404
44758
T135
G
S
G
G
D
G
T
T
L
P
T
E
T
S
P
Frog
Xenopus laevis
Q5M7E3
406
46042
T155
N
S
K
L
T
C
F
T
F
S
R
G
D
I
S
Zebra Danio
Brachydanio rerio
Q4KMK0
367
41244
T133
P
D
P
V
R
I
P
T
Q
S
H
H
T
G
W
Tiger Blowfish
Takifugu rubipres
Fruit Fly
Dros. melanogaster
NP_608573
503
57519
T250
P
E
H
L
S
L
Y
T
Y
E
M
G
N
C
S
Honey Bee
Apis mellifera
XP_394395
493
56346
T243
P
E
H
L
H
V
Y
T
F
E
R
D
N
Y
T
Nematode Worm
Caenorhab. elegans
NP_490958
465
51501
S212
P
Q
A
L
H
V
R
S
F
D
R
A
V
L
L
Sea Urchin
Strong. purpuratus
XP_792601
513
57508
E135
D
T
P
E
D
A
P
E
D
A
P
E
N
G
N
Poplar Tree
Populus trichocarpa
Maize
Zea mays
Rice
Oryza sativa
Thale Cress
Arabidopsis thaliana
Baker's Yeast
Sacchar. cerevisiae
Red Bread Mold
Neurospora crassa
Conservation
Percent
Protein Identity:
100
44.7
96.6
84.9
N.A.
85.9
83.5
N.A.
38.5
61.1
59.3
35.1
N.A.
32.5
32.4
31.3
35.4
Protein Similarity:
100
45.9
98.4
91.4
N.A.
91.9
90.6
N.A.
47.9
74.5
72.6
54.1
N.A.
48.3
47.6
46.8
49.3
P-Site Identity:
100
100
86.6
86.6
N.A.
6.6
6.6
N.A.
0
6.6
66.6
13.3
N.A.
26.6
26.6
26.6
0
P-Site Similarity:
100
100
93.3
93.3
N.A.
6.6
6.6
N.A.
13.3
6.6
73.3
20
N.A.
60
60
40
13.3
Percent
Protein Identity:
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
Protein Similarity:
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
P-Site Identity:
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
P-Site Similarity:
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
Phosphosite
Consensus
Position
-7
-6
-5
-4
-3
-4
-5
0
+1
+2
+3
+4
+5
+6
+7
% Ala:
8
0
8
0
0
8
0
0
0
22
0
8
0
0
0
% A
% Cys:
0
0
15
0
0
36
0
0
0
0
0
0
0
8
0
% C
% Asp:
8
8
0
0
15
8
0
0
8
36
0
8
36
0
8
% D
% Glu:
0
15
0
8
0
0
0
8
0
15
0
15
0
0
0
% E
% Phe:
0
0
0
15
8
15
36
0
50
0
0
0
8
15
0
% F
% Gly:
8
0
8
8
0
8
0
0
15
0
0
43
0
22
0
% G
% His:
0
0
15
0
15
0
0
0
0
0
8
8
0
0
0
% H
% Ile:
0
0
0
0
0
8
0
0
0
0
0
0
0
22
0
% I
% Lys:
0
0
8
0
0
0
0
8
0
0
0
0
0
0
0
% K
% Leu:
15
8
0
58
0
8
0
0
8
0
0
0
0
8
8
% L
% Met:
0
0
0
0
0
0
0
0
0
0
8
0
0
0
0
% M
% Asn:
8
0
29
8
0
0
0
8
0
0
0
0
22
0
8
% N
% Pro:
29
29
15
0
0
0
15
0
0
8
8
0
0
0
22
% P
% Gln:
0
8
8
0
0
0
0
0
8
0
0
8
0
0
0
% Q
% Arg:
0
15
0
0
36
0
8
8
0
0
50
0
15
0
0
% R
% Ser:
29
15
0
0
8
0
15
8
0
22
0
15
0
8
43
% S
% Thr:
0
8
0
0
22
0
8
65
0
0
8
0
15
0
8
% T
% Val:
0
0
0
8
0
15
8
0
0
0
22
0
8
15
0
% V
% Trp:
0
0
0
0
0
0
0
0
0
0
0
0
0
0
8
% W
% Tyr:
0
0
0
0
0
0
15
0
15
0
0
0
0
8
0
% Y
% Spaces:
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
% _