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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: NSUN4 All Species: 16.67
Human Site: S149 Identified Species: 28.21
UniProt: Q96CB9 Number Species: 13
    Phosphosite Substitution
    Charge Score: 0.08
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens Q96CB9 NP_950245.2 384 43089 S149 F P P A R P G S L G V M E Y Y
Chimpanzee Pan troglodytes XP_513142 833 91470 S149 F P P A R P G S L G V M E Y Y
Rhesus Macaque Macaca mulatta XP_001108301 384 43099 S149 F P P A R P G S L G V M D Y Y
Dog Lupus familis XP_853047 384 43059 S149 F P P A R L G S L G V M D Y Y
Cat Felis silvestris
Mouse Mus musculus Q9CZ57 381 42835 L149 A R L G S L G L M D Y Y L M D
Rat Rattus norvegicus NP_001100148 381 42594 L149 A S V G S L G L M D Y Y L M D
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001518738 248 26969 L36 W P S I R V G L L S E Q K Y G
Chicken Gallus gallus XP_422449 404 44758 N151 L R A S L S S N V K C Y T F P
Frog Xenopus laevis Q5M7E3 406 46042 C171 F P Q S R S D C F G L L E Y Y
Zebra Danio Brachydanio rerio Q4KMK0 367 41244 A149 K Q Y Y L L N A A S L L P V L
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_608573 503 57519 M266 F K G P K K C M T G V L S H F
Honey Bee Apis mellifera XP_394395 493 56346 S259 F P N P K K G S T G V L D Y Y
Nematode Worm Caenorhab. elegans NP_490958 465 51501 E228 F P A P M K D E V G V P S Y W
Sea Urchin Strong. purpuratus XP_792601 513 57508 N151 A V E E S I S N P G S T G L D
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 44.7 96.6 84.9 N.A. 85.9 83.5 N.A. 38.5 61.1 59.3 35.1 N.A. 32.5 32.4 31.3 35.4
Protein Similarity: 100 45.9 98.4 91.4 N.A. 91.9 90.6 N.A. 47.9 74.5 72.6 54.1 N.A. 48.3 47.6 46.8 49.3
P-Site Identity: 100 100 93.3 86.6 N.A. 6.6 6.6 N.A. 33.3 0 46.6 0 N.A. 20 53.3 33.3 6.6
P-Site Similarity: 100 100 100 93.3 N.A. 13.3 13.3 N.A. 46.6 26.6 66.6 20 N.A. 46.6 73.3 46.6 13.3
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 22 0 15 29 0 0 0 8 8 0 0 0 0 0 0 % A
% Cys: 0 0 0 0 0 0 8 8 0 0 8 0 0 0 0 % C
% Asp: 0 0 0 0 0 0 15 0 0 15 0 0 22 0 22 % D
% Glu: 0 0 8 8 0 0 0 8 0 0 8 0 22 0 0 % E
% Phe: 58 0 0 0 0 0 0 0 8 0 0 0 0 8 8 % F
% Gly: 0 0 8 15 0 0 58 0 0 65 0 0 8 0 8 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 0 8 0 % H
% Ile: 0 0 0 8 0 8 0 0 0 0 0 0 0 0 0 % I
% Lys: 8 8 0 0 15 22 0 0 0 8 0 0 8 0 0 % K
% Leu: 8 0 8 0 15 29 0 22 36 0 15 29 15 8 8 % L
% Met: 0 0 0 0 8 0 0 8 15 0 0 29 0 15 0 % M
% Asn: 0 0 8 0 0 0 8 15 0 0 0 0 0 0 0 % N
% Pro: 0 58 29 22 0 22 0 0 8 0 0 8 8 0 8 % P
% Gln: 0 8 8 0 0 0 0 0 0 0 0 8 0 0 0 % Q
% Arg: 0 15 0 0 43 0 0 0 0 0 0 0 0 0 0 % R
% Ser: 0 8 8 15 22 15 15 36 0 15 8 0 15 0 0 % S
% Thr: 0 0 0 0 0 0 0 0 15 0 0 8 8 0 0 % T
% Val: 0 8 8 0 0 8 0 0 15 0 50 0 0 8 0 % V
% Trp: 8 0 0 0 0 0 0 0 0 0 0 0 0 0 8 % W
% Tyr: 0 0 8 8 0 0 0 0 0 0 15 22 0 58 43 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _