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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: MLH1 All Species: 4.24
Human Site: S446 Identified Species: 7.78
UniProt: P40692 Number Species: 12
    Phosphosite Substitution
    Charge Score: 0.17
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P40692 NP_000240.1 756 84601 S446 E V A A K N Q S L E G D T T K
Chimpanzee Pan troglodytes XP_001170274 756 84655 S446 E V T A K N Q S L E G D T T K
Rhesus Macaque Macaca mulatta XP_001087274 583 64794 K286 V Y A A Y L P K N T H P F L Y
Dog Lupus familis XP_534219 757 84567 E447 A A T N Q G M E E E T T E G T
Cat Felis silvestris
Mouse Mus musculus Q9JK91 760 84662 E450 A A E S E N L E R E S L M E T
Rat Rattus norvegicus P97679 757 84522 E448 A A D S A S L E R E S V I G A
Wallaby Macropus eugenll
Platypus Ornith. anatinus
Chicken Gallus gallus XP_418828 757 84832 D447 A D L I E M A D V Q Q D A V M
Frog Xenopus laevis NP_001090545 750 84121 A442 L D V L E A T A N S E S Y V L
Zebra Danio Brachydanio rerio NP_956953 724 80793 A427 D D A E L L T A A D V E P C G
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera XP_001120100 716 81818 H416 Q Q K D K S A H V I D N E K N
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_793318 745 83320 R435 D D G Q T L P R Y L K K N R K
Poplar Tree Populus trichocarpa XP_002325965 747 83627 L428 A G R L H A Y L Q A R P V D N
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P38920 769 87044 Q454 L T L N E S E Q P R D A N T I
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99.5 76 93 N.A. 88.1 87 N.A. N.A. 80 75.1 69.7 N.A. N.A. 50.6 N.A. 60.3
Protein Similarity: 100 99.8 76.5 96.1 N.A. 93.9 93.3 N.A. N.A. 88.1 85.9 81 N.A. N.A. 70.7 N.A. 75.7
P-Site Identity: 100 93.3 13.3 6.6 N.A. 13.3 6.6 N.A. N.A. 6.6 0 6.6 N.A. N.A. 6.6 N.A. 6.6
P-Site Similarity: 100 93.3 13.3 13.3 N.A. 26.6 20 N.A. N.A. 26.6 13.3 33.3 N.A. N.A. 33.3 N.A. 13.3
Percent
Protein Identity: 34.7 N.A. N.A. N.A. 36 N.A.
Protein Similarity: 56.6 N.A. N.A. N.A. 58.5 N.A.
P-Site Identity: 0 N.A. N.A. N.A. 6.6 N.A.
P-Site Similarity: 0 N.A. N.A. N.A. 26.6 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 39 24 24 24 8 16 16 16 8 8 0 8 8 0 8 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 8 0 % C
% Asp: 16 31 8 8 0 0 0 8 0 8 16 24 0 8 0 % D
% Glu: 16 0 8 8 31 0 8 24 8 39 8 8 16 8 0 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 0 0 8 0 0 % F
% Gly: 0 8 8 0 0 8 0 0 0 0 16 0 0 16 8 % G
% His: 0 0 0 0 8 0 0 8 0 0 8 0 0 0 0 % H
% Ile: 0 0 0 8 0 0 0 0 0 8 0 0 8 0 8 % I
% Lys: 0 0 8 0 24 0 0 8 0 0 8 8 0 8 24 % K
% Leu: 16 0 16 16 8 24 16 8 16 8 0 8 0 8 8 % L
% Met: 0 0 0 0 0 8 8 0 0 0 0 0 8 0 8 % M
% Asn: 0 0 0 16 0 24 0 0 16 0 0 8 16 0 16 % N
% Pro: 0 0 0 0 0 0 16 0 8 0 0 16 8 0 0 % P
% Gln: 8 8 0 8 8 0 16 8 8 8 8 0 0 0 0 % Q
% Arg: 0 0 8 0 0 0 0 8 16 8 8 0 0 8 0 % R
% Ser: 0 0 0 16 0 24 0 16 0 8 16 8 0 0 0 % S
% Thr: 0 8 16 0 8 0 16 0 0 8 8 8 16 24 16 % T
% Val: 8 16 8 0 0 0 0 0 16 0 8 8 8 16 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 8 0 0 8 0 8 0 8 0 0 0 8 0 8 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _