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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: TKT All Species: 20.91
Human Site: S548 Identified Species: 35.38
UniProt: P29401 Number Species: 13
    Phosphosite Substitution
    Charge Score: 0.15
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P29401 NP_001055.1 623 67878 S548 D R K L I L D S A R A T K G R
Chimpanzee Pan troglodytes Q5R1W6 623 67821 S548 D R K L I L D S A R A T K G R
Rhesus Macaque Macaca mulatta XP_001095403 703 76522 S629 D A A T I I S S A K A T G G R
Dog Lupus familis XP_533792 619 67627 S544 D R N L I L E S A R A T K G R
Cat Felis silvestris
Mouse Mus musculus P40142 623 67612 S548 D R K L I L D S A R A T K G R
Rat Rattus norvegicus P50137 623 67625 C548 D K K L I L D C A R A T K G R
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001508404 725 77819 S650 D K K L I L D S A K A T K G R
Chicken Gallus gallus XP_414333 627 68436 N548 D K K T I L E N A R A T K G R
Frog Xenopus laevis NP_001083312 627 67992 N548 D K K T I V E N A K A T N G H
Zebra Danio Brachydanio rerio XP_685690 628 69366 H549 D N K T I V D H V R A T R G R
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera XP_623357 627 67899 N553 D A Q L I V K N A K E V G G R
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_001177524 625 66606 C552 D A A T L T A C A K A A G G R
Poplar Tree Populus trichocarpa
Maize Zea mays Q7SIC9 675 72975 D603 F V S W E L F D E Q S D E Y K
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P23254 680 73787 E616 N V P I M S V E V L A T T C W
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99.3 61.1 87.3 N.A. 94.6 94 N.A. 74.6 84.2 78.3 71 N.A. N.A. 62.2 N.A. 61.1
Protein Similarity: 100 99.6 72.2 91.8 N.A. 97.9 97.7 N.A. 81.2 92.3 90.4 84.5 N.A. N.A. 76.2 N.A. 76.3
P-Site Identity: 100 100 53.3 86.6 N.A. 100 86.6 N.A. 86.6 73.3 46.6 60 N.A. N.A. 40 N.A. 33.3
P-Site Similarity: 100 100 66.6 93.3 N.A. 100 93.3 N.A. 100 93.3 80 73.3 N.A. N.A. 66.6 N.A. 46.6
Percent
Protein Identity: N.A. 25.3 N.A. N.A. 25.4 N.A.
Protein Similarity: N.A. 42.3 N.A. N.A. 41.1 N.A.
P-Site Identity: N.A. 6.6 N.A. N.A. 13.3 N.A.
P-Site Similarity: N.A. 33.3 N.A. N.A. 33.3 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 22 15 0 0 0 8 0 79 0 86 8 0 0 0 % A
% Cys: 0 0 0 0 0 0 0 15 0 0 0 0 0 8 0 % C
% Asp: 86 0 0 0 0 0 43 8 0 0 0 8 0 0 0 % D
% Glu: 0 0 0 0 8 0 22 8 8 0 8 0 8 0 0 % E
% Phe: 8 0 0 0 0 0 8 0 0 0 0 0 0 0 0 % F
% Gly: 0 0 0 0 0 0 0 0 0 0 0 0 22 86 0 % G
% His: 0 0 0 0 0 0 0 8 0 0 0 0 0 0 8 % H
% Ile: 0 0 0 8 79 8 0 0 0 0 0 0 0 0 0 % I
% Lys: 0 29 58 0 0 0 8 0 0 36 0 0 50 0 8 % K
% Leu: 0 0 0 50 8 58 0 0 0 8 0 0 0 0 0 % L
% Met: 0 0 0 0 8 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 8 8 8 0 0 0 0 22 0 0 0 0 8 0 0 % N
% Pro: 0 0 8 0 0 0 0 0 0 0 0 0 0 0 0 % P
% Gln: 0 0 8 0 0 0 0 0 0 8 0 0 0 0 0 % Q
% Arg: 0 29 0 0 0 0 0 0 0 50 0 0 8 0 79 % R
% Ser: 0 0 8 0 0 8 8 43 0 0 8 0 0 0 0 % S
% Thr: 0 0 0 36 0 8 0 0 0 0 0 79 8 0 0 % T
% Val: 0 15 0 0 0 22 8 0 15 0 0 8 0 0 0 % V
% Trp: 0 0 0 8 0 0 0 0 0 0 0 0 0 0 8 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 0 8 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _