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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: PFKFB1 All Species: 22.42
Human Site: S21 Identified Species: 44.85
UniProt: P16118 Number Species: 11
    Phosphosite Substitution
    Charge Score: -0.09
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P16118 NP_002616.2 471 54681 S21 Q K I W I P H S S G S S R L Q
Chimpanzee Pan troglodytes XP_001158340 470 54094 N21 K I W M P Y S N G R P A L H A
Rhesus Macaque Macaca mulatta XP_001091907 471 54591 S21 Q K I W I P H S S G S S R L H
Dog Lupus familis XP_549023 589 67657 S139 Q K I W I P H S S G S S G L Q
Cat Felis silvestris
Mouse Mus musculus P70266 471 54831 S21 Q K I W I P H S S S S S L L Q
Rat Rattus norvegicus P07953 471 54745 S21 Q K I W I P H S S S S S V L Q
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001509392 470 54778 T21 Q K I W I P H T S N R L Q Q R
Chicken Gallus gallus Q91348 470 54386 S22 Q K V W V P L S L H R L R R R
Frog Xenopus laevis NP_001088535 470 54750 Q21 Q K I W I P H Q C E R L Q Q R
Zebra Danio Brachydanio rerio XP_683102 465 54169 R23 M S S R L R Q R R G S S V P Q
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q21122 457 52072 G21 V A H S D E H G F S D Q V R V
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P32604 452 52576 P22 C V I M V G L P A R G K S F I
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 74.5 98.5 78.7 N.A. 95.5 95.3 N.A. 90.8 79.8 82.1 78.3 N.A. N.A. N.A. 49.2 N.A.
Protein Similarity: 100 85.3 99.3 79.4 N.A. 97.4 97.2 N.A. 95.7 88.7 90.8 89.3 N.A. N.A. N.A. 67 N.A.
P-Site Identity: 100 0 93.3 93.3 N.A. 86.6 86.6 N.A. 53.3 40 46.6 26.6 N.A. N.A. N.A. 6.6 N.A.
P-Site Similarity: 100 20 93.3 93.3 N.A. 86.6 86.6 N.A. 73.3 60 60 33.3 N.A. N.A. N.A. 6.6 N.A.
Percent
Protein Identity: N.A. N.A. N.A. N.A. 44.1 N.A.
Protein Similarity: N.A. N.A. N.A. N.A. 64.1 N.A.
P-Site Identity: N.A. N.A. N.A. N.A. 6.6 N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. 20 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 9 0 0 0 0 0 0 9 0 0 9 0 0 9 % A
% Cys: 9 0 0 0 0 0 0 0 9 0 0 0 0 0 0 % C
% Asp: 0 0 0 0 9 0 0 0 0 0 9 0 0 0 0 % D
% Glu: 0 0 0 0 0 9 0 0 0 9 0 0 0 0 0 % E
% Phe: 0 0 0 0 0 0 0 0 9 0 0 0 0 9 0 % F
% Gly: 0 0 0 0 0 9 0 9 9 34 9 0 9 0 0 % G
% His: 0 0 9 0 0 0 67 0 0 9 0 0 0 9 9 % H
% Ile: 0 9 67 0 59 0 0 0 0 0 0 0 0 0 9 % I
% Lys: 9 67 0 0 0 0 0 0 0 0 0 9 0 0 0 % K
% Leu: 0 0 0 0 9 0 17 0 9 0 0 25 17 42 0 % L
% Met: 9 0 0 17 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 0 0 0 0 0 0 9 0 9 0 0 0 0 0 % N
% Pro: 0 0 0 0 9 67 0 9 0 0 9 0 0 9 0 % P
% Gln: 67 0 0 0 0 0 9 9 0 0 0 9 17 17 42 % Q
% Arg: 0 0 0 9 0 9 0 9 9 17 25 0 25 17 25 % R
% Ser: 0 9 9 9 0 0 9 50 50 25 50 50 9 0 0 % S
% Thr: 0 0 0 0 0 0 0 9 0 0 0 0 0 0 0 % T
% Val: 9 9 9 0 17 0 0 0 0 0 0 0 25 0 9 % V
% Trp: 0 0 9 67 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 9 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _