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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
Separase
Full Name:
Separin
Alias:
Caspase-like protein ESPL1; ESP1; ESPL1; Extra spindle pole bodies 1; Extra spindle poles-like 1 protein; KIAA0165
Type:
EC 3.4.22.49; Protease
Mass (Da):
233113
Number AA:
2120
UniProt ID:
Q14674
International Prot ID:
IPI00017538
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0005813
GO:0005634
Uniprot
OncoNet
Molecular Function:
GO:0008234
GO:0005515
PhosphoSite+
KinaseNET
Biological Process:
GO:0006915
GO:0000910
GO:0040001
Phosida
TranscriptoNet
STRING
Kinexus Products
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S3
_
_
_
_
_
M
R
S
F
K
R
V
N
F
G
Site 2
T11
F
K
R
V
N
F
G
T
L
L
S
S
Q
K
E
Site 3
S31
P
D
L
K
E
F
L
S
N
P
P
A
G
F
P
Site 4
S39
N
P
P
A
G
F
P
S
S
R
S
D
A
E
R
Site 5
S40
P
P
A
G
F
P
S
S
R
S
D
A
E
R
R
Site 6
S42
A
G
F
P
S
S
R
S
D
A
E
R
R
Q
A
Site 7
Y85
A
E
L
A
C
D
G
Y
L
V
S
T
P
Q
R
Site 8
S88
A
C
D
G
Y
L
V
S
T
P
Q
R
P
P
L
Site 9
T89
C
D
G
Y
L
V
S
T
P
Q
R
P
P
L
Y
Site 10
Y96
T
P
Q
R
P
P
L
Y
L
E
R
I
L
F
V
Site 11
S113
R
N
A
A
A
Q
G
S
P
E
V
T
L
R
L
Site 12
T117
A
Q
G
S
P
E
V
T
L
R
L
A
Q
P
L
Site 13
Y140
R
E
A
A
P
Q
D
Y
E
A
V
A
R
G
S
Site 14
S147
Y
E
A
V
A
R
G
S
F
S
L
L
W
K
G
Site 15
S182
L
V
L
L
E
D
E
S
T
P
C
E
V
P
H
Site 16
T183
V
L
L
E
D
E
S
T
P
C
E
V
P
H
F
Site 17
S238
A
L
V
G
E
R
G
S
S
S
G
L
L
S
P
Site 18
S239
L
V
G
E
R
G
S
S
S
G
L
L
S
P
Q
Site 19
S240
V
G
E
R
G
S
S
S
G
L
L
S
P
Q
R
Site 20
S273
R
H
H
D
K
A
I
S
A
V
E
K
A
H
S
Site 21
S280
S
A
V
E
K
A
H
S
Y
L
R
N
T
N
L
Site 22
Y281
A
V
E
K
A
H
S
Y
L
R
N
T
N
L
A
Site 23
T285
A
H
S
Y
L
R
N
T
N
L
A
P
S
L
Q
Site 24
S323
I
K
A
S
A
V
L
S
K
S
M
E
A
P
S
Site 25
S325
A
S
A
V
L
S
K
S
M
E
A
P
S
P
P
Site 26
S330
S
K
S
M
E
A
P
S
P
P
L
R
A
L
Y
Site 27
T351
L
S
G
L
E
R
G
T
K
R
R
Y
R
L
D
Site 28
Y355
E
R
G
T
K
R
R
Y
R
L
D
A
I
L
S
Site 29
Y383
Q
L
R
D
D
G
V
Y
G
G
S
S
K
Q
Q
Site 30
S392
G
S
S
K
Q
Q
Q
S
F
L
Q
M
Y
F
Q
Site 31
Y397
Q
Q
S
F
L
Q
M
Y
F
Q
G
L
H
L
Y
Site 32
T456
L
T
D
H
M
G
M
T
A
S
Y
T
S
N
L
Site 33
Y465
S
Y
T
S
N
L
A
Y
S
F
Y
S
H
K
L
Site 34
T494
L
G
L
V
K
P
G
T
Y
P
E
V
P
P
E
Site 35
Y495
G
L
V
K
P
G
T
Y
P
E
V
P
P
E
K
Site 36
S513
C
F
R
L
Q
V
E
S
L
K
K
L
G
K
Q
Site 37
S538
L
A
A
L
Q
P
C
S
P
E
H
M
A
E
P
Site 38
T568
D
K
E
L
Q
L
K
T
L
R
D
S
L
S
G
Site 39
S572
Q
L
K
T
L
R
D
S
L
S
G
W
D
P
E
Site 40
S574
K
T
L
R
D
S
L
S
G
W
D
P
E
T
L
Site 41
Y592
L
R
E
E
L
Q
A
Y
K
A
V
R
A
D
T
Site 42
T599
Y
K
A
V
R
A
D
T
G
Q
E
R
F
N
I
Site 43
S614
I
C
D
L
L
E
L
S
P
E
E
T
P
A
G
Site 44
T618
L
E
L
S
P
E
E
T
P
A
G
A
W
A
R
Site 45
S662
E
A
L
Q
L
L
D
S
V
R
P
E
A
Q
A
Site 46
Y719
F
E
V
N
D
L
N
Y
E
D
K
L
Q
E
D
Site 47
Y730
L
Q
E
D
R
F
L
Y
S
N
I
A
F
N
L
Site 48
S731
Q
E
D
R
F
L
Y
S
N
I
A
F
N
L
A
Site 49
S810
S
E
R
L
K
D
H
S
K
A
A
G
S
S
C
Site 50
Y831
L
T
L
G
C
P
S
Y
A
Q
L
H
L
E
E
Site 51
S841
L
H
L
E
E
A
A
S
S
L
K
H
L
D
Q
Site 52
S842
H
L
E
E
A
A
S
S
L
K
H
L
D
Q
T
Site 53
T849
S
L
K
H
L
D
Q
T
T
D
T
Y
L
L
L
Site 54
T850
L
K
H
L
D
Q
T
T
D
T
Y
L
L
L
S
Site 55
T852
H
L
D
Q
T
T
D
T
Y
L
L
L
S
L
T
Site 56
Y868
D
L
L
R
S
Q
L
Y
W
T
H
Q
K
V
T
Site 57
Y898
Q
K
S
S
K
A
W
Y
L
L
R
V
Q
V
L
Site 58
S916
A
A
Y
L
S
L
P
S
N
N
L
S
H
S
L
Site 59
S920
S
L
P
S
N
N
L
S
H
S
L
W
E
Q
L
Site 60
S922
P
S
N
N
L
S
H
S
L
W
E
Q
L
C
A
Site 61
S969
Q
K
A
A
V
E
T
S
F
L
D
Y
G
E
N
Site 62
Y973
V
E
T
S
F
L
D
Y
G
E
N
L
V
Q
K
Site 63
S985
V
Q
K
W
Q
V
L
S
E
V
L
S
C
S
E
Site 64
S1073
G
V
T
Q
H
L
D
S
V
K
K
V
H
L
Q
Site 65
T1111
P
A
L
E
L
V
A
T
V
A
K
E
P
G
P
Site 66
S1122
E
P
G
P
I
A
P
S
T
N
S
S
P
V
L
Site 67
T1123
P
G
P
I
A
P
S
T
N
S
S
P
V
L
K
Site 68
S1125
P
I
A
P
S
T
N
S
S
P
V
L
K
T
K
Site 69
S1126
I
A
P
S
T
N
S
S
P
V
L
K
T
K
P
Site 70
T1199
P
E
A
A
E
R
L
T
Q
A
L
Q
A
S
L
Site 71
T1210
Q
A
S
L
N
H
K
T
P
P
S
L
V
P
S
Site 72
S1213
L
N
H
K
T
P
P
S
L
V
P
S
L
L
D
Site 73
S1239
L
E
G
L
N
Q
P
S
N
E
S
L
Q
K
V
Site 74
S1242
L
N
Q
P
S
N
E
S
L
Q
K
V
L
Q
S
Site 75
S1301
W
Q
P
P
L
I
K
S
V
P
G
S
E
P
S
Site 76
S1305
L
I
K
S
V
P
G
S
E
P
S
K
T
Q
G
Site 77
T1310
P
G
S
E
P
S
K
T
Q
G
Q
K
R
S
G
Site 78
S1316
K
T
Q
G
Q
K
R
S
G
R
G
R
Q
K
L
Site 79
S1325
R
G
R
Q
K
L
A
S
A
P
L
S
L
N
N
Site 80
S1329
K
L
A
S
A
P
L
S
L
N
N
T
S
Q
K
Site 81
T1333
A
P
L
S
L
N
N
T
S
Q
K
G
L
E
G
Site 82
S1334
P
L
S
L
N
N
T
S
Q
K
G
L
E
G
R
Site 83
T1346
E
G
R
G
L
P
C
T
P
K
P
P
D
R
I
Site 84
T1363
A
G
P
H
V
P
F
T
V
F
E
E
V
C
P
Site 85
T1371
V
F
E
E
V
C
P
T
E
S
K
P
E
V
P
Site 86
S1373
E
E
V
C
P
T
E
S
K
P
E
V
P
Q
A
Site 87
T1389
R
V
Q
Q
R
V
Q
T
R
L
K
V
N
F
S
Site 88
S1396
T
R
L
K
V
N
F
S
D
D
S
D
L
E
D
Site 89
S1399
K
V
N
F
S
D
D
S
D
L
E
D
P
V
S
Site 90
S1406
S
D
L
E
D
P
V
S
A
E
A
W
L
A
E
Site 91
T1420
E
E
P
K
R
R
G
T
A
S
R
G
R
G
R
Site 92
S1422
P
K
R
R
G
T
A
S
R
G
R
G
R
A
R
Site 93
T1436
R
K
G
L
S
L
K
T
D
A
V
V
A
P
G
Site 94
S1455
N
P
G
L
N
G
R
S
R
R
A
K
K
V
A
Site 95
S1475
E
R
R
P
Q
R
A
S
D
Q
A
R
P
G
P
Site 96
T1487
P
G
P
E
I
M
R
T
I
P
E
E
E
L
T
Site 97
T1494
T
I
P
E
E
E
L
T
D
N
W
R
K
M
S
Site 98
S1501
T
D
N
W
R
K
M
S
F
E
I
L
R
G
S
Site 99
S1508
S
F
E
I
L
R
G
S
D
G
E
D
S
A
S
Site 100
S1513
R
G
S
D
G
E
D
S
A
S
G
G
K
T
P
Site 101
S1515
S
D
G
E
D
S
A
S
G
G
K
T
P
A
P
Site 102
T1519
D
S
A
S
G
G
K
T
P
A
P
G
P
E
A
Site 103
S1538
W
E
L
L
R
L
D
S
S
K
K
K
L
P
S
Site 104
S1539
E
L
L
R
L
D
S
S
K
K
K
L
P
S
P
Site 105
S1545
S
S
K
K
K
L
P
S
P
C
P
D
K
E
S
Site 106
S1552
S
P
C
P
D
K
E
S
D
K
D
L
G
P
R
Site 107
Y1614
C
L
G
H
R
D
P
Y
A
T
A
F
L
V
T
Site 108
T1616
G
H
R
D
P
Y
A
T
A
F
L
V
T
E
S
Site 109
T1634
T
C
R
H
Q
L
L
T
H
L
H
R
Q
L
S
Site 110
S1641
T
H
L
H
R
Q
L
S
K
A
Q
K
H
R
G
Site 111
S1649
K
A
Q
K
H
R
G
S
L
E
I
A
D
Q
L
Site 112
S1660
A
D
Q
L
Q
G
L
S
L
Q
E
M
P
G
D
Site 113
S1678
A
R
I
Q
R
L
F
S
F
R
A
L
E
S
G
Site 114
S1694
F
P
Q
P
E
K
E
S
F
Q
E
R
L
A
L
Site 115
S1734
L
T
R
L
E
K
D
S
P
P
V
S
V
Q
I
Site 116
S1738
E
K
D
S
P
P
V
S
V
Q
I
P
T
G
Q
Site 117
S1768
Q
K
A
Q
K
E
N
S
S
C
T
D
K
R
E
Site 118
S1769
K
A
Q
K
E
N
S
S
C
T
D
K
R
E
W
Site 119
T1771
Q
K
E
N
S
S
C
T
D
K
R
E
W
W
T
Site 120
S1810
K
G
L
L
L
P
S
S
E
E
P
G
P
A
Q
Site 121
Y1833
L
Q
D
C
G
W
K
Y
P
D
R
T
L
L
K
Site 122
T1837
G
W
K
Y
P
D
R
T
L
L
K
I
M
L
S
Site 123
T1884
V
G
R
L
Q
G
L
T
V
P
S
N
S
H
L
Site 124
S1940
Y
G
A
S
P
V
L
S
Q
G
V
D
P
R
S
Site 125
S1947
S
Q
G
V
D
P
R
S
T
F
Y
V
L
N
P
Site 126
T1948
Q
G
V
D
P
R
S
T
F
Y
V
L
N
P
H
Site 127
Y1950
V
D
P
R
S
T
F
Y
V
L
N
P
H
N
N
Site 128
S1959
L
N
P
H
N
N
L
S
S
T
E
E
Q
F
R
Site 129
S1960
N
P
H
N
N
L
S
S
T
E
E
Q
F
R
A
Site 130
S1970
E
Q
F
R
A
N
F
S
S
E
A
G
W
R
G
Site 131
S1971
Q
F
R
A
N
F
S
S
E
A
G
W
R
G
V
Site 132
Y1998
A
L
T
K
H
D
L
Y
I
Y
A
G
H
G
A
Site 133
Y2000
T
K
H
D
L
Y
I
Y
A
G
H
G
A
G
A
Site 134
T2065
L
G
N
L
W
D
V
T
D
R
D
I
D
R
Y
Site 135
Y2072
T
D
R
D
I
D
R
Y
T
E
A
L
L
Q
G
Site 136
T2073
D
R
D
I
D
R
Y
T
E
A
L
L
Q
G
W
Site 137
Y2091
G
P
G
A
P
L
L
Y
Y
V
N
Q
A
R
Q
Site 138
Y2092
P
G
A
P
L
L
Y
Y
V
N
Q
A
R
Q
A
Site 139
S2118
I
A
Y
G
L
P
V
S
L
R
_
_
_
_
_
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation