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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
ACTN1
Full Name:
Alpha-actinin-1
Alias:
AAC1; Actinin, alpha 1; Alpha-actinin cytoskeletal isoform; F-actin cross linking protein; Non-muscle alpha-actinin 1
Type:
Cytoskeletal protein
Mass (Da):
103058
Number AA:
892
UniProt ID:
P12814
International Prot ID:
IPI00013508
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0030018
GO:0005856
GO:0005576
Uniprot
OncoNet
Molecular Function:
GO:0003779
GO:0005509
GO:0005178
PhosphoSite+
KinaseNET
Biological Process:
GO:0048041
GO:0051271
GO:0042981
Phosida
TranscriptoNet
STRING
Kinexus Products
Alpha-actinin-1 Y246 phosphosite-specific antibody AB-PN502#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PN502#Alpha-actinin-1 ( R133-K147, human) peptide - Powder PE-01AEX95#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01AEX95#Alpha-actinin-1 (M174-Q188, human) peptide - Powder PE-01AFS80#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01AFS80#Alpha-actinin-1 (S243-F249, human) pY246 phosphopeptide - Powder PE-04AIC99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AIC99
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
Y4
_
_
_
_
M
D
H
Y
D
S
Q
Q
T
N
D
Site 2
S6
_
_
M
D
H
Y
D
S
Q
Q
T
N
D
Y
M
Site 3
T9
D
H
Y
D
S
Q
Q
T
N
D
Y
M
Q
P
E
Site 4
Y12
D
S
Q
Q
T
N
D
Y
M
Q
P
E
E
D
W
Site 5
T50
S
H
L
R
K
A
G
T
Q
I
E
N
I
E
E
Site 6
S73
M
L
L
L
E
V
I
S
G
E
R
L
A
K
P
Site 7
S91
K
M
R
V
H
K
I
S
N
V
N
K
A
L
D
Site 8
T123
V
D
G
N
V
K
M
T
L
G
M
I
W
T
I
Site 9
S140
R
F
A
I
Q
D
I
S
V
E
E
T
S
A
K
Site 10
T144
Q
D
I
S
V
E
E
T
S
A
K
E
G
L
L
Site 11
Y161
C
Q
R
K
T
A
P
Y
K
N
V
N
I
Q
N
Site 12
Y193
H
R
P
E
L
I
D
Y
G
K
L
R
K
D
D
Site 13
T203
L
R
K
D
D
P
L
T
N
L
N
T
A
F
D
Site 14
Y215
A
F
D
V
A
E
K
Y
L
D
I
P
K
M
L
Site 15
T230
D
A
E
D
I
V
G
T
A
R
P
D
E
K
A
Site 16
T240
P
D
E
K
A
I
M
T
Y
V
S
S
F
Y
H
Site 17
Y241
D
E
K
A
I
M
T
Y
V
S
S
F
Y
H
A
Site 18
S243
K
A
I
M
T
Y
V
S
S
F
Y
H
A
F
S
Site 19
S244
A
I
M
T
Y
V
S
S
F
Y
H
A
F
S
G
Site 20
Y246
M
T
Y
V
S
S
F
Y
H
A
F
S
G
A
Q
Alpha-actinin-1 Y246 phosphosite-specific antibody AB-PN502#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PN502#Alpha-actinin-1 (S243-F249, human) pY246 phosphopeptide - Powder PE-04AIC99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AIC99
Site 21
S250
S
S
F
Y
H
A
F
S
G
A
Q
K
A
E
T
Site 22
T257
S
G
A
Q
K
A
E
T
A
A
N
R
I
C
K
Site 23
Y279
N
E
Q
L
M
E
D
Y
E
K
L
A
S
D
L
Site 24
S284
E
D
Y
E
K
L
A
S
D
L
L
E
W
I
R
Site 25
T293
L
L
E
W
I
R
R
T
I
P
W
L
E
N
R
Site 26
Y319
K
L
E
D
F
R
D
Y
R
R
L
H
K
P
P
Site 27
S348
L
Q
T
K
L
R
L
S
N
R
P
A
F
M
P
Site 28
S356
N
R
P
A
F
M
P
S
E
G
R
M
V
S
D
Site 29
S362
P
S
E
G
R
M
V
S
D
I
N
N
A
W
G
Site 30
S404
E
K
F
R
Q
K
A
S
I
H
E
A
W
T
D
Site 31
Y422
A
M
L
R
Q
K
D
Y
E
T
A
T
L
S
E
Site 32
T424
L
R
Q
K
D
Y
E
T
A
T
L
S
E
I
K
Site 33
S428
D
Y
E
T
A
T
L
S
E
I
K
A
L
L
K
Site 34
S442
K
K
H
E
A
F
E
S
D
L
A
A
H
Q
D
Site 35
Y466
Q
E
L
N
E
L
D
Y
Y
D
S
P
S
V
N
Site 36
Y467
E
L
N
E
L
D
Y
Y
D
S
P
S
V
N
A
Site 37
S469
N
E
L
D
Y
Y
D
S
P
S
V
N
A
R
C
Site 38
S471
L
D
Y
Y
D
S
P
S
V
N
A
R
C
Q
K
Site 39
T490
W
D
N
L
G
A
L
T
Q
K
R
R
E
A
L
Site 40
Y511
L
E
T
I
D
Q
L
Y
L
E
Y
A
K
R
A
Site 41
Y582
V
S
K
I
V
Q
T
Y
H
V
N
M
A
G
T
Site 42
T594
A
G
T
N
P
Y
T
T
I
T
P
Q
E
I
N
Site 43
T596
T
N
P
Y
T
T
I
T
P
Q
E
I
N
G
K
Site 44
T619
P
R
R
D
Q
A
L
T
E
E
H
A
R
Q
Q
Site 45
S657
M
E
E
I
G
R
I
S
I
E
M
H
G
T
L
Site 46
T663
I
S
I
E
M
H
G
T
L
E
D
Q
L
S
H
Site 47
S669
G
T
L
E
D
Q
L
S
H
L
R
Q
Y
E
K
Site 48
Y674
Q
L
S
H
L
R
Q
Y
E
K
S
I
V
N
Y
Site 49
S677
H
L
R
Q
Y
E
K
S
I
V
N
Y
K
P
K
Site 50
Y681
Y
E
K
S
I
V
N
Y
K
P
K
I
D
Q
L
Site 51
Y708
F
D
N
K
H
T
N
Y
T
M
E
H
I
R
V
Site 52
T737
E
V
E
N
Q
I
L
T
R
D
A
K
G
I
S
Site 53
S744
T
R
D
A
K
G
I
S
Q
E
Q
M
N
E
F
Site 54
S763
N
H
F
D
R
D
H
S
G
T
L
G
P
E
E
Site 55
T765
F
D
R
D
H
S
G
T
L
G
P
E
E
F
K
Site 56
S817
Q
A
F
I
D
F
M
S
R
E
T
A
D
T
D
Site 57
T820
I
D
F
M
S
R
E
T
A
D
T
D
T
A
D
Site 58
T823
M
S
R
E
T
A
D
T
D
T
A
D
Q
V
M
Site 59
T825
R
E
T
A
D
T
D
T
A
D
Q
V
M
A
S
Site 60
S832
T
A
D
Q
V
M
A
S
F
K
I
L
A
G
D
Site 61
Y842
I
L
A
G
D
K
N
Y
I
T
M
D
E
L
R
Site 62
Y859
L
P
P
D
Q
A
E
Y
C
I
A
R
M
A
P
Site 63
Y867
C
I
A
R
M
A
P
Y
T
G
P
D
S
V
P
Site 64
T868
I
A
R
M
A
P
Y
T
G
P
D
S
V
P
G
Site 65
S872
A
P
Y
T
G
P
D
S
V
P
G
A
L
D
Y
Site 66
Y887
M
S
F
S
T
A
L
Y
G
E
S
D
L
_
_
Site 67
S890
S
T
A
L
Y
G
E
S
D
L
_
_
_
_
_
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation