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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
HGK
Full Name:
Mitogen-activated protein kinase kinase kinase kinase 4
Alias:
EC 2.7.11.1; FLH21957; Hepatocyte progenitor kinase-like/germinal center kinase-like kinase; HPK/GCK-like kinase; HPK/GCK-like kinase HGK; M4K4; MAP4K4; MAPK kinase kinase kinase 4; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; MEKKK4; Mitogen-activated protein kinase kinase kinase kinase 4; Nck interacting kinase; NIK
Type:
EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; MSN subfamily
Mass (Da):
142101
Number AA:
1239
UniProt ID:
O95819
International Prot ID:
IPI00006752
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
Uniprot
OncoNet
Molecular Function:
GO:0005524
GO:0004674
GO:0005083
PhosphoSite+
KinaseNET
Biological Process:
GO:0006468
GO:0007243
GO:0006950
Phosida
TranscriptoNet
STRING
Kinexus Products
Mitogen-activated protein kinase kinase kinase kinase 4 pan-specific antibody AB-NK300-1#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-NK300-1#Mitogen-activated protein kinase kinase kinase kinase 4 T187 phosphosite-specific antibody AB-PK653#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK653#Mitogen-activated protein kinase kinase kinase kinase 4 (A2-D20, human) peptide - Powder PE-01BFM99T#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01BFM99T#Mitogen-activated protein kinase kinase kinase kinase 4 (R184-G190, human) pT187 phosphopeptide - Powder PE-04ALN99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04ALN99
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S5
_
_
_
M
A
N
D
S
P
A
K
S
L
V
D
Site 2
S9
A
N
D
S
P
A
K
S
L
V
D
I
D
L
S
Site 3
S16
S
L
V
D
I
D
L
S
S
L
R
D
P
A
G
Site 4
T35
V
E
V
V
G
N
G
T
Y
G
Q
V
Y
K
G
Site 5
Y36
E
V
V
G
N
G
T
Y
G
Q
V
Y
K
G
R
Site 6
Y40
N
G
T
Y
G
Q
V
Y
K
G
R
H
V
K
T
Site 7
T59
A
I
K
V
M
D
V
T
E
D
E
E
E
E
I
Site 8
Y76
E
I
N
M
L
K
K
Y
S
H
H
R
N
I
A
Site 9
Y85
H
H
R
N
I
A
T
Y
Y
G
A
F
I
K
K
Site 10
Y86
H
R
N
I
A
T
Y
Y
G
A
F
I
K
K
S
Site 11
S93
Y
G
A
F
I
K
K
S
P
P
G
H
D
D
Q
Site 12
Y132
L
K
E
D
W
I
A
Y
I
S
R
E
I
L
R
Site 13
T162
K
G
Q
N
V
L
L
T
E
N
A
E
V
K
L
Site 14
S175
K
L
V
D
F
G
V
S
A
Q
L
D
R
T
V
Site 15
T181
V
S
A
Q
L
D
R
T
V
G
R
R
N
T
F
Site 16
T187
R
T
V
G
R
R
N
T
F
I
G
T
P
Y
W
Mitogen-activated protein kinase kinase kinase kinase 4 T187 phosphosite-specific antibody AB-PK653#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK653#Mitogen-activated protein kinase kinase kinase kinase 4 (R184-G190, human) pT187 phosphopeptide - Powder PE-04ALN99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04ALN99
Site 17
T191
R
R
N
T
F
I
G
T
P
Y
W
M
A
P
E
Site 18
Y193
N
T
F
I
G
T
P
Y
W
M
A
P
E
V
I
Site 19
Y210
D
E
N
P
D
A
T
Y
D
Y
R
S
D
L
W
Site 20
Y212
N
P
D
A
T
Y
D
Y
R
S
D
L
W
S
C
Site 21
S255
N
P
P
P
R
L
K
S
K
K
W
S
K
K
F
Site 22
S259
R
L
K
S
K
K
W
S
K
K
F
F
S
F
I
Site 23
S264
K
W
S
K
K
F
F
S
F
I
E
G
C
L
V
Site 24
Y274
E
G
C
L
V
K
N
Y
M
Q
R
P
S
T
E
Site 25
S279
K
N
Y
M
Q
R
P
S
T
E
Q
L
L
K
H
Site 26
T280
N
Y
M
Q
R
P
S
T
E
Q
L
L
K
H
P
Site 27
T309
L
K
D
H
I
D
R
T
R
K
K
R
G
E
K
Site 28
T319
K
R
G
E
K
D
E
T
E
Y
E
Y
S
G
S
Site 29
Y321
G
E
K
D
E
T
E
Y
E
Y
S
G
S
E
E
Site 30
Y323
K
D
E
T
E
Y
E
Y
S
G
S
E
E
E
E
Site 31
S324
D
E
T
E
Y
E
Y
S
G
S
E
E
E
E
E
Site 32
S326
T
E
Y
E
Y
S
G
S
E
E
E
E
E
E
V
Site 33
S341
P
E
Q
E
G
E
P
S
S
I
V
N
V
P
G
Site 34
S342
E
Q
E
G
E
P
S
S
I
V
N
V
P
G
E
Site 35
S350
I
V
N
V
P
G
E
S
T
L
R
R
D
F
L
Site 36
T351
V
N
V
P
G
E
S
T
L
R
R
D
F
L
R
Site 37
Y387
Q
L
R
E
Q
E
E
Y
K
R
Q
L
L
A
E
Site 38
Y467
R
V
E
R
E
Q
E
Y
I
R
R
Q
L
E
E
Site 39
Y520
Q
L
Q
Q
E
Q
A
Y
L
L
S
L
Q
H
D
Site 40
S523
Q
E
Q
A
Y
L
L
S
L
Q
H
D
H
R
R
Site 41
S536
R
R
P
H
P
Q
H
S
Q
Q
P
P
P
P
Q
Site 42
S547
P
P
P
Q
Q
E
R
S
K
P
S
F
H
A
P
Site 43
S550
Q
Q
E
R
S
K
P
S
F
H
A
P
E
P
K
Site 44
Y560
A
P
E
P
K
A
H
Y
E
P
A
D
R
A
R
Site 45
T576
V
E
D
R
F
R
K
T
N
H
S
S
P
E
A
Site 46
S579
R
F
R
K
T
N
H
S
S
P
E
A
Q
S
K
Site 47
S580
F
R
K
T
N
H
S
S
P
E
A
Q
S
K
Q
Site 48
S598
V
L
E
P
P
V
P
S
R
S
E
S
F
S
N
Site 49
S600
E
P
P
V
P
S
R
S
E
S
F
S
N
G
N
Site 50
S602
P
V
P
S
R
S
E
S
F
S
N
G
N
S
E
Site 51
S604
P
S
R
S
E
S
F
S
N
G
N
S
E
S
V
Site 52
S608
E
S
F
S
N
G
N
S
E
S
V
H
P
A
L
Site 53
S610
F
S
N
G
N
S
E
S
V
H
P
A
L
Q
R
Site 54
T627
E
P
Q
V
P
V
R
T
T
S
R
S
P
V
L
Site 55
T628
P
Q
V
P
V
R
T
T
S
R
S
P
V
L
S
Site 56
S629
Q
V
P
V
R
T
T
S
R
S
P
V
L
S
R
Site 57
S631
P
V
R
T
T
S
R
S
P
V
L
S
R
R
D
Site 58
S635
T
S
R
S
P
V
L
S
R
R
D
S
P
L
Q
Site 59
S639
P
V
L
S
R
R
D
S
P
L
Q
G
S
G
Q
Site 60
S644
R
D
S
P
L
Q
G
S
G
Q
Q
N
S
Q
A
Site 61
S649
Q
G
S
G
Q
Q
N
S
Q
A
G
Q
R
N
S
Site 62
S656
S
Q
A
G
Q
R
N
S
T
S
I
E
P
R
L
Site 63
T657
Q
A
G
Q
R
N
S
T
S
I
E
P
R
L
L
Site 64
S658
A
G
Q
R
N
S
T
S
I
E
P
R
L
L
W
Site 65
S677
K
L
V
P
R
P
G
S
G
S
S
S
G
S
S
Site 66
S679
V
P
R
P
G
S
G
S
S
S
G
S
S
N
S
Site 67
S680
P
R
P
G
S
G
S
S
S
G
S
S
N
S
G
Site 68
S681
R
P
G
S
G
S
S
S
G
S
S
N
S
G
S
Site 69
S683
G
S
G
S
S
S
G
S
S
N
S
G
S
Q
P
Site 70
S684
S
G
S
S
S
G
S
S
N
S
G
S
Q
P
G
Site 71
S686
S
S
S
G
S
S
N
S
G
S
Q
P
G
S
H
Site 72
S688
S
G
S
S
N
S
G
S
Q
P
G
S
H
P
G
Site 73
S692
N
S
G
S
Q
P
G
S
H
P
G
S
Q
S
G
Site 74
S696
Q
P
G
S
H
P
G
S
Q
S
G
S
G
E
R
Site 75
S698
G
S
H
P
G
S
Q
S
G
S
G
E
R
F
R
Site 76
S700
H
P
G
S
Q
S
G
S
G
E
R
F
R
V
R
Site 77
S708
G
E
R
F
R
V
R
S
S
S
K
S
E
G
S
Site 78
S709
E
R
F
R
V
R
S
S
S
K
S
E
G
S
P
Site 79
S710
R
F
R
V
R
S
S
S
K
S
E
G
S
P
S
Site 80
S712
R
V
R
S
S
S
K
S
E
G
S
P
S
Q
R
Site 81
S715
S
S
S
K
S
E
G
S
P
S
Q
R
L
E
N
Site 82
S717
S
K
S
E
G
S
P
S
Q
R
L
E
N
A
V
Site 83
T743
P
L
K
P
A
D
L
T
A
L
A
K
E
L
R
Site 84
T762
V
R
P
P
H
K
V
T
D
Y
S
S
S
S
E
Site 85
Y764
P
P
H
K
V
T
D
Y
S
S
S
S
E
E
S
Site 86
S765
P
H
K
V
T
D
Y
S
S
S
S
E
E
S
G
Site 87
S766
H
K
V
T
D
Y
S
S
S
S
E
E
S
G
T
Site 88
S767
K
V
T
D
Y
S
S
S
S
E
E
S
G
T
T
Site 89
S768
V
T
D
Y
S
S
S
S
E
E
S
G
T
T
D
Site 90
S771
Y
S
S
S
S
E
E
S
G
T
T
D
E
E
D
Site 91
T773
S
S
S
E
E
S
G
T
T
D
E
E
D
D
D
Site 92
T774
S
S
E
E
S
G
T
T
D
E
E
D
D
D
V
Site 93
T790
Q
E
G
A
D
E
S
T
S
G
P
E
D
T
R
Site 94
S791
E
G
A
D
E
S
T
S
G
P
E
D
T
R
A
Site 95
T796
S
T
S
G
P
E
D
T
R
A
A
S
S
L
N
Site 96
S800
P
E
D
T
R
A
A
S
S
L
N
L
S
N
G
Site 97
S801
E
D
T
R
A
A
S
S
L
N
L
S
N
G
E
Site 98
S805
A
A
S
S
L
N
L
S
N
G
E
T
E
S
V
Site 99
T809
L
N
L
S
N
G
E
T
E
S
V
K
T
M
I
Site 100
S811
L
S
N
G
E
T
E
S
V
K
T
M
I
V
H
Site 101
T814
G
E
T
E
S
V
K
T
M
I
V
H
D
D
V
Site 102
S823
I
V
H
D
D
V
E
S
E
P
A
M
T
P
S
Site 103
T828
V
E
S
E
P
A
M
T
P
S
K
E
G
T
L
Site 104
S830
S
E
P
A
M
T
P
S
K
E
G
T
L
I
V
Site 105
T834
M
T
P
S
K
E
G
T
L
I
V
R
Q
T
Q
Site 106
T840
G
T
L
I
V
R
Q
T
Q
S
A
S
S
T
L
Site 107
S842
L
I
V
R
Q
T
Q
S
A
S
S
T
L
Q
K
Site 108
S844
V
R
Q
T
Q
S
A
S
S
T
L
Q
K
H
K
Site 109
T846
Q
T
Q
S
A
S
S
T
L
Q
K
H
K
S
S
Site 110
S852
S
T
L
Q
K
H
K
S
S
S
S
F
T
P
F
Site 111
S853
T
L
Q
K
H
K
S
S
S
S
F
T
P
F
I
Site 112
S854
L
Q
K
H
K
S
S
S
S
F
T
P
F
I
D
Site 113
S855
Q
K
H
K
S
S
S
S
F
T
P
F
I
D
P
Site 114
T857
H
K
S
S
S
S
F
T
P
F
I
D
P
R
L
Site 115
S868
D
P
R
L
L
Q
I
S
P
S
S
G
T
T
V
Site 116
S870
R
L
L
Q
I
S
P
S
S
G
T
T
V
T
S
Site 117
T896
E
A
I
R
Q
D
P
T
R
K
G
S
V
V
N
Site 118
S900
Q
D
P
T
R
K
G
S
V
V
N
V
N
P
T
Site 119
T907
S
V
V
N
V
N
P
T
N
T
R
P
Q
S
D
Site 120
S913
P
T
N
T
R
P
Q
S
D
T
P
E
I
R
K
Site 121
T915
N
T
R
P
Q
S
D
T
P
E
I
R
K
Y
K
Site 122
Y921
D
T
P
E
I
R
K
Y
K
K
R
F
N
S
E
Site 123
S927
K
Y
K
K
R
F
N
S
E
I
L
C
A
A
L
Site 124
Y959
R
S
G
Q
G
K
V
Y
P
L
I
N
R
R
R
Site 125
T981
E
G
L
N
V
L
V
T
I
S
G
K
K
D
K
Site 126
Y992
K
K
D
K
L
R
V
Y
Y
L
S
W
L
R
N
Site 127
Y993
K
D
K
L
R
V
Y
Y
L
S
W
L
R
N
K
Site 128
S995
K
L
R
V
Y
Y
L
S
W
L
R
N
K
I
L
Site 129
T1016
E
K
K
Q
G
W
T
T
V
G
D
L
E
G
C
Site 130
Y1026
D
L
E
G
C
V
H
Y
K
V
V
K
Y
E
R
Site 131
Y1048
L
K
S
S
V
E
V
Y
A
W
A
P
K
P
Y
Site 132
S1063
H
K
F
M
A
F
K
S
F
G
E
L
V
H
K
Site 133
T1077
K
P
L
L
V
D
L
T
V
E
E
G
Q
R
L
Site 134
Y1088
G
Q
R
L
K
V
I
Y
G
S
C
A
G
F
H
Site 135
Y1105
D
V
D
S
G
S
V
Y
D
I
Y
L
P
T
H
Site 136
Y1143
C
Y
E
D
E
G
V
Y
V
N
T
Y
G
R
I
Site 137
Y1147
E
G
V
Y
V
N
T
Y
G
R
I
T
K
D
V
Site 138
Y1167
E
M
P
T
S
V
A
Y
I
R
S
N
Q
T
M
Site 139
S1185
E
K
A
I
E
I
R
S
V
E
T
G
H
L
D
Site 140
S1217
N
D
K
V
F
F
A
S
V
R
S
G
G
S
S
Site 141
S1224
S
V
R
S
G
G
S
S
Q
V
Y
F
M
T
L
Site 142
Y1227
S
G
G
S
S
Q
V
Y
F
M
T
L
G
R
T
Site 143
T1230
S
S
Q
V
Y
F
M
T
L
G
R
T
S
L
L
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation